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Biomedical subjects

A M Khan

Publications and source records attributed to A M Khan.

At least 109 records · Page 6Linked to original sources
PubMed1987

Detection of flavivirus RNA in infected cells using photobiotin-labelled hybridization probes.

Ten plasmids containing viral cDNA inserts of portions of the dengue virus type 2 (DEN-2) or Kunjin virus (KUN) genomes were biotinylated using photobiotin acetate and used as probes for the detection of flavivirus RNA in infected Vero cells. The viral cDNA inserts ranged in length from 0.19 to 2.7 kilobase pairs, and represented segments of the flavivirus genome coding for structural and nonstructural proteins. In spot hybridization assays (hybridization at 60 degrees C) with RNA extracted from cells infected with one of fourteen different flaviviruses or Semliki Forest virus, all DEN-2 and KUN probes hybridized specifically with RNA from cells infected with DEN-2 or KUN, respectively. At the reduced stringency of lower temperatures, specific hybridization to homologous viral RNA was still a feature of the probes, and only limited cross-hybridization to the RNA of some other flavivirus species was detected.

Animals↗
PubMed1984

Age related detection of tissue amyloid P in the skin.

The presence of tissue amyloid P component in normal skin from fetuses and children was studied using direct, fluorescent, immunohistochemical techniques on frozen sections. Tissue amyloid P is not detected in fetuses or in children up to the age of 2 years but from the age of 4 years upwards it is readily detected and invariably present. Though the function of human amyloid P is unknown its role appears to be age or maturity related.

Adolescent↗
PubMed1981

A Japanese encephalitis focus in Bangladesh.

This paper describes a focus of Japanese encephalitis in Modhupur Forest area, Bangladesh. The disease was diagnosed for the first time in Bangladesh. The diagnosis was based on the clinical picture of the disease and the results of serological investigations; the causative agent could not be isolated. During 2 year's surveillance of the focus, no new cases of the disease were recorded. Only 1.9% of 1046 healthy persons tested showed HI antibodies against group B arboviruses, and no virus was isolated from 41 mosquito pools. These results indicate that the disease was introduced into the area, produced an outbreak and later died out.

Adolescent↗
PubMed1979

Sub-populations of peripheral lymphocytes from patients with shigella bacillary dysentery with associated leucocytosis.

In this study we ascertained the proportion and absolute number of T, B and nul cell peripheral lymphocytes from Shigella dysenteriae type I patients and compared these results with those from age matched controls. Single blood samples were collected from 13 Bangladeshi children who complained of symptoms for one or more weeks. Patients were divided into two categories, with and without a leukaemoid response. The leukaemoid response was primarily granulocytic in nature, but there was a substantial increase in the mean number of lymphocytes. Over one third of the lymphocytes were nul cells. These cells rose proportionally to the decrease of T-cells, as the proportion of B-cells remained within normal range. One leukaemoid patient died. The absolute number of lymphocytes was normal and the B-cells were normal in respect of both proportions and absolute numbers. T-cell percentage and numbers were well below normal values.

B-Lymphocytes↗
PubMed1977

Histochemical observations on the mycetomes of Pyrilla perpusilla Walker.

Histochemical studies of mycetomes and mycetocytes of Pyrilla perpusilla show PAS positive material, what can suggest the presence of glycogen. The mycetocytes failed to stain with alcian blue and methyl green pyronin "Y" indicating the absence of mucopolysaccharides and RNA respectively. The mycetocytes show positive congo red. Millon and Mercury bromophenophenol blue staining thereby proving that they contain glycoprotein, tyrosine and proteins. Positive reaction with Sudan black may show the presence of lipids and lipoproteins.

Animals↗
PubMed1976

Histochemical observation of the mycetomes of Idiocerus clypealis Leth.

Histochemical tests on the mycetomes and mycetocytes of Idiocerus clypealis show positive PAS and Congo red reactions for the presence of glycogen and glycoprotein (amyloid). The mycetocytes failed to take alcian blue and methyl green pyronin Y stains showing the absence of mucopolysaccharides and RNA respectively. Positive millions and bromophenol blue reactions prove that they contain tyrosine and general proteins. The presence of lipids and lipoprotein is shown by a positive stain with sudan black B.

Animals↗
PubMed1975

In vitro and in vivo evaluation of change of local pH on bacteria in C.S.O.M.

An in vitro study has shown a high bactericidal activity of 2 per cent acetic acid. An in vitro study in 400 cases of C.S.O.M. has shown that conservative treatment of C.S.O.M. by pH change with 2 per cent acetic acid is better than antibiotic ear drops alone. However, combined therapy (pH change plus antibiotic ear drops) is definitely superior to single treatment by either of the two methods. Two per cent acetic acid is well tolerated by the middle ear mucosa except in 1 per cent cases, where its use had to be discontinued because of irritation. The use of 2 per cent acetic acid is highly recommended, particularly for poor nations, because of its negligible cost as compared to antibiotic ear drops.

Acetates↗