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Biomedical subjects

A M Jullien

Publications and source records attributed to A M Jullien.

34 records · Page 2Linked to original sources
PubMed1987

[Prevalence of anti-HIV antibodies in immunoglobulins recipients].

Seven hundred and forty subjects who, between 1980 and 1985, had received intravenous or intramuscular injections of immunoglobulins (Ig) prepared by Cohn fractionation of plasma pools from more than 2000 donors were investigated for anti-HIV antibodies. Anti-HIV antibodies were detected in only one subject, a female drug addict and therefore belonging to a group at high risk of AIDS. In contrast with the huge amounts of Ig received by the remaining 739 subjects (976 litres intravenously, 128 litres intramuscularly), this young woman had only received 10 ml of anti-HBs Ig intramuscularly. The wide scattering in both space and time of these recipients of Ig and the prevalence of anti-HIV antibodies among blood donors in France (0.7 per 1000 in 1985) suggest that all the batches studied (over 100) were contaminated by HIV. The absence in these subjects of seropositive reaction ascribable to the injections of Ig confirms that Cohn fractionation inactivates the virus, as demonstrated in vitro. It is concluded that recipients of Ig prepared from plasma pools not tested for the absence of anti-HIV antibodies should not be regarded as a group at risk of AIDS.

Acquired Immunodeficiency Syndrome↗
PubMed1981

[Technical, immunological and clinical study of the performance of blood filtration using Erypur filters].

Technical, immunological and clinical parameters of blood filtration through Erypur filters were evaluated. Red cell concentrates from which the buffy coat had not been eliminated were used throughout the study. Filtration procedure is fully automated, simple and takes about 15 minutes. 98% of leukocytes and 96% of platelets were found to be retained by the filter. Residual leukocytes were less than 3.10(7) in 68% and residual platelets less than 1.10(10) in 93% of filtered units. The incidence of lymphocyte alloantibody formation was 14.9% in the group of 67 patients who received 210 filtered units and 27.9% in the control group of 93 patients who were transfused with 298 non-filtered units. 330 filtered units were transfused to 107 carefully monitored patients and caused no reaction. 40 of these patients had polyspecific HLA antibodies and/or a documented history of non-hemolytic transfusion reactions. The clinical usefulness of Erypur filtered blood in the prevention of such reactions may thus be confirmed.

Blood↗
PubMedDate not supplied

Transmission of serologically silent hepatitis B virus along with hepatitis C virus in two cases of posttransfusion hepatitis.

The polymerase chain reaction (PCR) was used to investigate the presence of hepatitis B virus (HBV)-related DNA sequences in blood from three blood donors and two transfusion recipients who developed posttransfusion non-A, non-B hepatitis (NANBH). In the first case, the sole donor was positive for antibody to hepatitis B surface (HBs) and core (HBc) antigens and had elevated alanine aminotransferase (ALT) levels, while the recipient had no HBV serologic markers. Both the donor and the recipient had serologic markers of hepatitis C virus (HCV) and were found positive for HBV DNA and HCV RNA sequences by PCR. The second case involved two donors and one recipient. Serologic tests for conventional HBV markers were negative in all three individuals, but one of the donors had elevated ALT. HBV DNA sequences were detected by PCR in the serum of the recipient and of the donor with high ALT, but not in the serum of the donor with normal ALT. Anti-HCV was detected in the serum of the recipient and of the suspect donor but not in that of the donor with normal ALT. The sequences amplified in the S region and determined after cloning of PCR products for both donor-recipient pairs were indistinguishable from each other and identical to the sequence of the major HBV subtype of adw in the first case and ayw in the second case. Furthermore, for the second case, an identical single-point mutation was found in both the donor and the recipient. These data confirm the transmission of conserved HBV sequences together with HCV in posttransfusion NANBH.(ABSTRACT TRUNCATED AT 250 WORDS)

Alanine Transaminase↗