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Biomedical subjects

A M Gassel

Publications and source records attributed to A M Gassel.

46 records · Page 3Linked to original sources
PubMed1990

Human cardiac allograft rejection. Correlation of grading with expression of different monocyte/macrophage markers.

Endomyocardial biopsies from human cardiac allografts were investigated with the use of immunohistochemical methods. A correlation between the different degrees of rejection reaction and subtypes of monocyte/macrophage infiltrates was established. Early and mild rejection is characterized by recently immigrated monocytes/macrophages positive for the monoclonal antibody Ki-M1. In contrast, the cellular infiltrates in severe and resolving rejection show strong expression of antigens recognized by Ki-M6 and Ki-M8, indicating increased phagocytotic function of monocytes/macrophages. The immunohistochemical assessment of monocytes/macrophages can thus be used, in addition to conventional histology, in the diagnosis of cardiac allograft rejection.

Antibodies, Monoclonal↗
PubMedDate not supplied

Primary glomangioma of the liver: imaging findings.

Glomus organs are small arteriovenous anastomoses that are chiefly responsible for thermoregulation of the distal portion of the extremities. Glomangiomas are benign tumors of these bodies. Glomangiomas occur preferentially in the fingers and toes, but some case reports have described primary glomangiomas in the respiratory or gastrointestinal tract and the genitals. To date, no glomangiomas of the liver have been observed. We report on a 61-year-old patient in whom routine ultrasound disclosed a subcapsular well-defined mass in the liver. On imaging studies, no correspondence to the usual liver tumors was found. Magnetic resonance-guided biopsy showed a primary glomangioma of the liver. Clinically, the patient had no appetite and lost weight over several months. Due to the patient's weight loss and potential malignant transformation, the tumor was excised. Histologic work-up confirmed the diagnosis of a glomangioma with no signs of malignancy.

Biopsy↗
PubMedDate not supplied

[Ovarian epidermoid cyst and endometrioid carcinoma: do they share their origin?].

A case of a well differentiated endometrioid adenocarcinoma of the ovary in combination with an epidermoid cyst is described. A typical epidermoid cyst without skin appendages could be observed. In direct neighborhood, there was a typical endometrioid carcinoma with some foci of squamous metaplasia. There was no continuum between the squamous metaplasia of the carcinoma and the wall of the epidermoid cyst. In addition, the benign cyst and the carcinoma shared some of their immunohistochemical profile. This case highlights the still unsolved question about the origin of epidermoid cysts and adds to the hypothesis that they arise from pluripotent coelomic epithelium.

Endometrial Neoplasms↗
PubMedDate not supplied

The correlation of c-erbB-2 oncoprotein and established prognostic factors in human breast cancer.

BACKGROUND: The c-erbB-2 (HER2/neu) receptor is a transmembrane phosphoglycoprotein associated with multiple signal transduction pathways. Its overexpression in breast cancer tissue has been correlated with poor prognosis. We report preliminary data of an ongoing study in invasive breast cancer patients exploring c-erbB-2 protein overexpression in relation to established tumor characteristics of prognostic value. MATERIALS AND METHODS: In primary breast carcinoma samples from 115 women undergoing surgery in our department in 1999, a polyclonal rabbit antibody to human c-erbB-2 oncoprotein was used for immunohistochemical assessment of the c-erbB-2 expression in formalin-fixed paraffin-embedded material. The data were statistically correlated with classical histopathological parameters. RESULTS: In the studied collective of mainly postmenopausal women (75%) with a high rate of early stage breast cancer (88% pT1 + 2), there was no significant relation between c-erbB-2 overexpression, classified as positive in 42% of the samples, and lymph node involvement, tumor size and grade, or hormone receptor status. CONCLUSION: Using the presented highly sensitive method, no association between c-erbB-2 expression and established prognostic factors was found. These data are in line with reports that the value of HER2/neu determination is not fully clarified for the preadjuvant evaluation of newly diagnosed breast cancer patients.

Adult↗
PubMedDate not supplied

Heat-shock protein 70 as a prognostic marker in node-negative breast cancer.

BACKGROUND: Heat-shock proteins (hsp) are involved in processes which are associated with tumour neogenesis and the biological behaviour of tumours. The object of our study was to investigate the significance of the 70 kDa hsp for the outcome of women with node-negative breast cancer. PATIENTS AND METHODS: Paraffin sections of 191 patients with operated breast cancer and a median follow-up of 177 months were stained immunohistochemically with a commercially available antibody against hsp70. RESULTS: We found a statistically significant correlation of the nuclear staining pattern with tumour size (> or < or = 2 cm; p = 0.046) and with a low tumour grading (G1 and G2 versus G3; p = 0.029). Patients showing a cytoplasmatic staining for hsp70 had a statistically significantly decreased overall survival [OS] (p = 0.04) and a shorter survival after recurrence [SR] (p = 0.026). CONCLUSION: The nuclear share of hsp70 is associated with various biological characteristics of malignant breast tumours, while the occurrence of cytoplasmatic hsp70 influences OS and SR.

Adult↗
PubMedDate not supplied

Heat shock protein 27 is associated with decreased survival in node-negative breast cancer patients.

BACKGROUND: Heat shock protein 27 (hsp27) is a molecular chaperone which supports cells to keep their homeostasis under stressful conditions. It is associated with resistance to chemotherapeutics, radiation and hyperthermia. The aim of this retrospective study was to investigate the prognostic value of hsp27 for patients with node-negative breast cancer. MATERIALS AND METHODS: Paraffin sections of 191 patients were stained immunohistochemically with a monoclonal antibody against hsp27. Median follow-up was 177 months. The results were correlated with clinical and histopathological parameters using the Chi-square test. RESULTS: There was no significant correlation between hsp27 expression and standard histopathological features or the proliferation marker ki-67. Disease-free survival (DFS) was not altered for patients expressing hsp27-positive tumors, whereas overall survival (OS) [p=0. 02] and survival after first recurrence (SR) [p=0.01] were significantly decreased. CONCLUSION: The expression of hsp27 in primary breast cancers is associated with a short survival for node-negative patients.

Adult↗