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Biomedical subjects

A M Bueno

Publications and source records attributed to A M Bueno.

4 recordsLinked to original sources

Frequencies of chromosomal aberrations in rodents collected in the coal-field and tobacco culture region of Criciúma, south Brazil.

The present work has been accomplished in the basin of the Sangão River (Criciúma, South Brazil), which receives the rejects from coal washing, mainly heavy metals. In the tobacco cultures at the river banks several agrotoxics are used. We tried to detect and estimate the possible action of this environmental pollution on rodents directly exposed to it through the study of chromosome aberration frequency in the bone marrow. The same methodology was applied to rodents collected from a nonpolluted area of the same state (Florianópolis, 190 km from Criciúma). The results obtained showed significant differences in total chromosome aberrations at the different sites. There is therefore a suggestion that this methodology could be applied to detect the action of environmental pollution on organisms.

Animals↗

Effects of perinatal hypothyroidism in the carbohydrate composition of cochlear tectorial membrane.

The presence of carbohydrates in the cochlear tectorial membrane (TM) of normal and hypothyroid rats was analyzed using fluorescent lectin probes. SBA and WGA lectins exhibited a similar reactivity in both normal and hypothyroid TMs. DBA, RCA1, UEA1 and Con A lectins were also reactive, although they showed a different distribution pattern between normal and hypothyroid TMs. Lastly, one of the lectins, PNA, was only labeled in hypothyroid TMs. These findings suggest that carbohydrate chains containing residues of N-acetyl-D-galactosamine (GalNAc) and N-acetyl-D-glucosamine, are similarly distributed in normal and hypothyroid TMs. Other carbohydrate residues as GalNAc alpha 1,3 GalNAc, D-galactose (Gal), L-fucose and D-mannose, are present, but are abnormally distributed in hypothyroid TMs. The Gal beta 1,3GalNAc residues, recognized by PNA, could be present only in the hypothyroid TMs. Alterations in glycosylation of the glycoproteins in the hypothyroid TM could be responsible for the abnormal distribution pattern of carbohydrate residues here described, and for the distorted shape of the hypothyroid TM.

Animals↗

[Overview of dermatophytes in Mali].

517 isolations of dermatophytes collected in Mali are reported. The various species are described and the main findings are: --Trichophyton species are more common (85.5% isolated from scalp lesions and 77.5% from skin scrapings) than Microsporum species. --Trichophyton verrucosum and Microsporum canis have been found found for the first time in Mali. --Ill-defined clinical forms of scalp lesions caused by either T. schonleinii or T. soudanense occur in the subsaharian areas of the country.

Adult↗