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Biomedical subjects

A M Bilous

Publications and source records attributed to A M Bilous.

28 records · Page 2Linked to original sources
PubMed1987

Whither cytosolic estrogen receptor assays? A comparison of commercially available kits for estrogen receptor assay.

Frozen tissue sections and cytosols from 89 specimens of breast and ovarian tumours have been assayed for the presence of estrogen receptor (ER) or related protein using four commercially available monoclonal antibody methods. These were estrogen receptor enzyme immunoassay (ER-EIA), estrogen receptor enzyme immunocytochemical assay (ER-ICA), ER D5 antigen immunoradiometric assay and ER D5 antigen immunocytochemical assay. The results have been compared with those obtained using a standard dextran coated charcoal steroid binding assay (ER-DCC). The correlation coefficient (r) between ER-DCC and ER-EIA results was 0.72 while that of both monoclonal antibody cytosol methods and their respective immunocytochemical assays was 0.66. ER-ICA gave additional valuable information concerning receptor heterogeneity in breast cancer sections. However, the correlation between ER D5 antigen assays and both ER-DCC and ER-EIA was weak (r less than 0.4). We conclude that there are a number of methodological advantages in using the kit systems including their ability to detect receptor presence in small tumour specimens (e.g., "Tru-cut" biopsies) but that their usefulness is limited by the current lack of widely available monoclonal based methods for the concurrent determination of progestogen receptor. We believe that, once these are available, immunocytochemical technology could offer an alternative method of determining the steroid receptor concentration in both ovarian and breast tumours, thus obviating the need for costly and time-consuming cytosolic methods, with their inherent difficulties of quality control.

Adolescent↗
PubMed1984

Oral patterns of acute and chronic graft-v-host disease.

Oral manifestations of graft-v-host disease (GVHD) are described in five recipients of allogeneic bone marrow transplants. It was clear that oral mucosal involvement occurred in both acute and chronic phases of the disease, and that recognizable patterns of presentation and course also occurred. The mechanisms of acute and chronic GVHD and the significance of oral mucosal and salivary gland involvement are discussed.

Acute Disease↗
PubMed1980

Legionnaires' disease in Wellington.

Legionella pneumophila was demonstrated by serological immunocytological and histological techniques in a Wellington man who died of a severe pneumonia. This is the first demonstration of disease produced by this organism in New Zealand.

Humans↗
PubMedDate not supplied

[Effects of different regimes of freezing and thawing on chemiluminescence of rat liver mitochondria].

The rate of Fe2+ activated lipid peroxidation was studied as affected by the different freezing-thawing regime. The level of peroxidation was judged by kinetics and superfeeble luminiscence intensity of mitochondria. It was shown that freezing up to -196 degrees C and subsequent thawing of the mitochondria suspended in a salty medium (KCl) results in an increase in the rate of lipid peroxidation. A more distinct response to the introduction of peroxidation activator (Fe2SO4) is observed in mitochondria, especially in the first 1-2 minutes after ion introduction - in the phase of "slow flash". Besides, there is an increase in the level of stationary luminescence (2-10, 12-22 min) of the frozen-thawed mitochondria in comparison with the control. Slow thawing of quickly or slowly frozen mitochondria results in a more considerable increase in the luminescence light sum, than in the case of rapid thawing. The least-luminescence intensity after freezing and thawing of mitochondria suspension was observed after the rapid freezing and rapid thawing. In case of slow thawing the rate of mitochondria freezing does not considerably affect the degree of chemiluminescence.

Animals↗
PubMedDate not supplied

[Importance of concentration factors in cryoinjury of lysosomes].

The injury degree of lysosomes isolated from the rat liver under low temperature conditions was studied with respect to nonsedimented hydrolases activities (acid RNases, DNase, phosphatase, cathepsins) taken as a biochemical criterium of lysosomal membrane stability. The lysosomal fraction was resuspended in 0.15 M NaCl solution and frozen in the liquid N2 vapours up to --30 degrees C. According to the thermographic analysis data the samples were thawed and studied at different stages of cooling. The activity of the studied hydrolases was established to increase most considerably during the water crystallization period and at the NaCl eutectic point. Hydrolytic enzymes release from lysosomes proved to be dependent on the thawing rate and NaCl concentration. The obtained data indicate to the importance of concentration factors in lysosomal membrane cryoinjury.

Cathepsins↗
PubMedDate not supplied

[Low temperature and cryoprotectant effect on oxygen uptake in rat kidney cortex homogenates].

Respiration and oxidative phosphorylation of the rat kidney cortex mitochondria are studied as affected by low temperatures and cryoprotectants. There is no change in respiration in state 2 during freezing and thawing of the rat kidney cortex slices both in case of using the succinate oxidation substrate and alpha-ketoglutarate. Respiration in state 3 is inhibited in the medium with alpha-ketoglutarate during freezing under protection of polyethylene oxide-100 (PEO-100) and glycerol, and does not significantly change in case of using dimethyl sulphoxide (DMSO). The effectiveness of cryoprotectants used in the experiments reduces in the following sequence: DMSO--glycerol--PEO-100. Preservation of the main mitochondria function, ATP synthesis, is shown to be possible for the kidney cortex slices exposed to freezing and thawing.

Adenosine Triphosphate↗