Biomedical subjects

A Leforestier

Publications and source records attributed to A Leforestier.

4 recordsLinked to original sources

PubMed1995

HOROPLAN: computer-assisted nurse scheduling using constraint-based programming.

Nurse scheduling is a difficult and time consuming task. The schedule has to determine the day to day shift assignments of each nurse for a specified period of time in a way that satisfies the given requirements as much as possible, taking into account the wishes of nurses as closely as possible. This paper presents a constraint-based, artificial intelligence approach by describing a prototype implementation developed with the Charme language and the first results of its use in the Rouen University Hospital. Horoplan implements a non-cyclical constraint-based scheduling, using some heuristics. Four levels of constraints were defined to give a maximum of flexibility: French level (e.g. number of worked hours in a year), hospital level (e.g. specific day-off), department level (e.g. specific shift) and care unit level (e.g. specific pattern for week-ends). Some constraints must always be verified and can not be overruled and some constraints can be overruled at a certain cost. Rescheduling is possible at any time specially in case of an unscheduled absence.

France↗

PubMed1995

DNA-DNA interaction in thin layer analysed by cryo-electron microscopy.

Thin vitrified films of 146 base pair double-stranded DNA fragments in semi-dilute solutions are observed by cryo-electron microscopy. Depending on the salt conditions of the buffer and the DNA concentration, the molecules form different characteristic arrangements: they are arranged either locally parallel to each other in random overall orientation or packed perpendicular to the film surface, or else are randomly oriented. These arrangements reflect the weak forces acting between the DNA molecules themselves, as well as between the molecules and the thin film surface.

Buffers↗

PubMed1993

Supramolecular ordering of DNA in the cholesteric liquid crystalline phase: an ultrastructural study.

Aqueous solutions of 146-base pair DNA fragments form a cholesteric liquid crystalline phase in the range of about 160-290 mg/ml. We present a structural analysis of this phase by comparing the data obtained from polarizing and electron microscopy. This phase shows multiple aspects or "textures" which are presented and interpreted. They mainly depend on the orientation of the structure relative to the observation plane and on the nature, distribution, and amount of defects present in the phase. These defects are then analyzed with the two methods, and the molecular orientations can be defined precisely in their core. The biological interest of such structural analyses is discussed in relation with the understanding of chromatin structure and function.

Biophysical Phenomena↗

PubMed1991

Cholesteric liquid crystalline DNA; a comparative analysis of cryofixation methods.

The ultrastructure of liquid crystalline phases of DNA raises numerous problems because of the structure itself which is fluid and which nature depends on the relative amount of DNA, water and ions. Different cryofixation methods were tested and compared after freeze-fracture of the specimen. A good ultrastructural preservation of the samples can be achieved without addition of any cryoprotectant by quick-freezing against a copper block cooled down to liquid helium temperature. Then, molecular orientations can be followed very accurately and the local disorder around a mean direction which exists in the liquid state is kept in the frozen structure.

Copper↗