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Biomedical subjects

A Lee

Publications and source records attributed to A Lee.

At least 685 records · Page 38Linked to original sources
PubMed1988

Transrectal US of the seminal vesicles and ejaculatory ducts: clinical correlation.

Transrectal ultrasonography (US) provides excellent anatomic detail of pathologic changes in the seminal vesicles and ejaculatory ducts. Fifty-two patients with US findings of seminal vesicle dilatation or cysts, ejaculatory duct cysts, or seminal vesicle or ejaculatory duct calculi were given questionnaires concerning a broad spectrum of genito-urinary symptoms. Compared with age-matched controls with normal US findings, patients with calculi in the seminal vesicles or ejaculatory ducts had a significantly increased prevalence of hematospermia and ejaculatory pain (P less than .01), and patients with cystic dilatation of the seminal vesicles were more likely to have perineal pain. Large midline cysts containing calculi or debris were symptomatic and probably represent müllerian duct remnants. Small cysts of the ejaculatory ducts were asymptomatic. Transrectal US may provide clinical insight into the causes of significant genitourinary symptoms that may previously have been ascribed to chronic nonbacterial prostatitis or have been considered to be idiopathic.

Calculi↗
PubMed1988

A Moloney MLV-rat somatotropin fusion gene produces biologically active somatotropin in a transgenic pig.

Expression of a Moloney murine leukemia virus (MLV) rat somatotropin fusion gene was examined in a transgenic pig. The fusion gene was integrated in a single site within the genome in a tandem array with approximately eight copies per cell. The integrated in a single site within the genome in a tandem array with approximately eight copies per cell. The integrated MLV-rat somatotropin fusion gene produced high levels of circulating rat somatotropin and resulted in an elevation in the circulating levels of insulin-like growth factor I. Although there was no increase in the rate of growth of the transgenic animal during the rapid growth phase, several phenotypic changes were evident. Skeletal growth was markedly increased and fat deposition was reduced throughout the animal. Blood glucose levels were elevated without ketosis. Northern blot analyses of rat somatotropin RNA revealed that expression of the fusion gene was highest in the spleen, lung, intestine, lymph nodes, and bone marrow. These results show that the MLV promoter can be used to express high levels of biologically active rat somatotropin in transgenic swine.

Animals↗
PubMed1988

Differences in the gastrointestinal microbiota of specific pathogen free mice: an often unknown variable in biomedical research.

Large differences were found in the numbers of facultatively anaerobic Gram-negative bacteria in the gastrointestinal tract of mice from 3 major specific pathogen free (SPF) units in Australia. The species isolated also differed between mouse colonies. In one unit the presence of Enterobacter cloacae was found to dramatically influence the survival of mice following total body irradiation. This finding conforms with previous studies which have shown the influence of variation in gastrointestinal microbiota on the immune system and on susceptibility to infection. Given that the presence or absence of Enterobacteriaceae in the intestines of mice under investigation may influence experimental results, researchers using SPF rodents are encouraged to determine the baseline loading of these bacteria in their animals. Where results of immunological or irradiation studies from different colonies are likely to be compared, the enterobacterial status of the colony being used should be reported.

Animals↗
PubMed1988

Patterns of plasma imipramine-desipramine concentrations in patients receiving concomitant fluphenazine decanoate.

Plasma levels of imipramine and desipramine were monitored in a series of 13 patients with postpsychotic depressions who had a fixed dose of imipramine (150 mg/day) added to their clinically adjusted, stable dose of fluphenazine decanoate. Despite the potential for metabolic inhibition of the tricyclic drug by the neuroleptic drug, no relationship was found between the fluphenazine dose and the subsequent plasma concentrations of imipramine and desipramine. However, plasma antidepressant levels after 1 week at a low dose (50 mg/day) identified patients who later generated high plasma antidepressant levels at the full antidepressant dosage. The clinical implications of these observations are discussed.

Adult↗
PubMed1988

Validating the components of a taxonomic system for rapists: a path analytic approach.

This study was part of a series designed to validate a classification system for rapists developed at the Massachusetts Treatment Center. This system (MTC:R2) determines subtypes according to three major dimensions: the meaning of the aggression in the rapist's offenses (expressive versus instrumental), the meaning of the sexual behavior (compensatory, exploitative, displaced anger, or sadistic), and the degree of general lifestyle impulsivity (low or high). A total of 201 rapists were subtyped using this system. A path analytic approach was used to relate family, juvenile, and adult histories to the main decisions of subtype assignment. The classification of an offender on each of the three dimensions was informed by a developmental perspective. Results provided support for the use of the discriminators used and highlighted the deficiencies in our current knowledge regarding developmental precursors. These results were integrated with the findings from other studies using MTC:R2 and suggestions for revisions to the current classification system were discussed.

Aggression↗
PubMed1988

Acute presentation of Campylobacter pylori gastritis.

A 46-yr-old male was admitted to hospital with profuse vomiting, epigastric pain, and an infiltrative gastric lesion. Evidence is presented supporting the proposition that the symptoms were the product of an acute inflammatory response in the stomach induced by the bacterium Campylobacter pylori. The progress of the patient was monitored over a 2-yr period by endoscopic examination and serology for C. pylori. A significant rise in IgG antibody to C. pylori was seen only at 14 wk.

Aged↗
PubMed1987

Waiting list statistics. III. Comparison of two measures of waiting times.

The length of time that patients spend on waiting lists is a topic of current concern. Calculating the proportion of patients who have been on a waiting list for a long time by taking a census of patients on the list at a single point in time will tend to yield a higher estimate than that obtained by calculating waiting times of patients admitted to hospital during a period of time. To illustrate this point the waiting times of patients in the Oxford region as measured by SBH 203 returns ("census" data) were compared with those as measured by the Hospital Activity Analysis ("event" data). As expected, the SBH 203 census returns showed a higher proportion of patients who had waited over a year compared with the "event" measure of all admissions. This difference, which is analagous to the difference between prevalence and incidence in epidemiology, should be considered when using data from these sources to calculate waiting times.

Appointments and Schedules↗
PubMed1987

Waiting list statistics. II: An estimate of inflation of waiting list length.

The discrepancy between the length of the waiting list and eventual admissions from the waiting list was investigated by comparing data from two different sources of routine statistics in the Oxford region. It was estimated that about 28% of the waiting list comprised patients who were not eventually admitted to hospital within the region.

Appointments and Schedules↗
PubMed1987

Waiting list statistics. I: Relation between admissions from waiting list and length of waiting list.

The relation between changes in inpatient workload, measured as increases or decreases in the number of inpatients admitted from the waiting list, and the overall length of the waiting list was studied. Overall trends in admissions from the waiting list, the influence of seasonal patterns, and the impact of industrial action on admissions were also studied. The hypothesis was that when admissions from the waiting list increased the length of the waiting list would decrease and vice versa. No such simple relation was found. In fact, if anything, as the number of admissions from the waiting list increased so did the length of the waiting list. This result could be due to inconsistencies in compiling waiting list data or to the use of waiting lists to improve organisational efficiency. It is also possible, and perhaps likely, that the ability to meet need in admitting patients to hospital influences patients and their doctors to translate previously unmet need into demand for hospital services.

Appointments and Schedules↗
PubMed1987

Cloning of the beta subunit of the leukocyte adhesion proteins: homology to an extracellular matrix receptor defines a novel supergene family.

We have isolated cDNA clones encoding the beta subunit of the human LFA-1, Mac-1, and p150,95 family of leukocyte adhesion proteins. The deduced 769-amino-acid sequence defines a cysteine-rich polypeptide with the characteristic features of an integral membrane protein. Peptide sequence data, Northern blot analysis, and Southern blot analysis suggest that a single gene encodes the beta subunit of all three leukocyte adhesion proteins. There is 45% homology between the beta subunit sequence and band III of integrin, a chick fibronectin and laminin receptor. This homology defines a new supergene family of cellular adhesion proteins.

Amino Acid Sequence↗
PubMed1987

Ventilatory effects of meptazinol and pethidine in anaesthetized patients.

The ventilatory effects of three doses of meptazinol (20 mg, 30 mg and 48 mg) were studied in anaesthetized patients and compared with those of pethidine 20 mg. Minute volume, tidal volume, ventilatory frequency, inspiratory and expiratory times were measured and mean inspiratory flow rates calculated. Each patient received a first dose of drug which was followed after 5 min by a second identical dose. After the first injection the ventilatory effects of meptazinol were dose-dependent and meptazinol 20 mg had an effect similar to pethidine 20 mg. Following repeated injection the effects on ventilation were markedly different between the drugs. Carbon dioxide retention and other compensatory mechanisms attenuate the ventilatory depression of meptazinol, but these may be inadequate in the anaesthetized patient.

Adult↗
PubMed1987

Evaluation of the efficacy of elastic compression stockings in prevention of hypotension during epidural anaesthesia for elective caesarean section.

The ability of graduated compression elastic stockings to prevent hypotension during elective epidural caesarean section was evaluated. Twenty women were randomly assigned to two groups of ten, one group being fitted with the stockings. The incidence and degree of hypotension were the same in both groups. Graduated compression elastic stockings are of no benefit in reducing the incidence of maternal hypotension during caesarean section.

Anesthesia, Epidural↗
PubMed1987

Demonstration of a flagellar antigen shared by a diverse group of spiral-shaped bacteria that colonize intestinal mucus.

Western blot analysis showed that there is little immunological cross-reactivity of the human pathogens Campylobacter jejuni and Campylobacter coli with Campylobacter pyloridis, except for a very strong cross-reaction between the flagellins. This same antigenic cross-reaction was found with two isolates of gram-negative spiral microaerophilic bacteria that are known to colonize the intestinal mucosa of rodents, but not with the flagellins of a number of other motile bacteria. It is proposed that this shared flagellin antigen may be important structurally and functionally.

Animals↗
PubMed1987

Cecectomy causes long-term reduction of colonization resistance in the mouse gastrointestinal tract.

Removal of the cecum from normal mice caused a major perturbation of the microbial ecology of the gastrointestinal tract. There was a permanent reduction in colonization resistance resulting in a 1,000-fold increase in the concentration of facultatively anaerobic coliform bacteria. The animals were significantly more susceptible to peroral challenge by the intestinal pathogen Salmonella enteritidis. Coincident with this increase in coliform counts and susceptibility to salmonellae was a decrease in the numbers of strictly anaerobic fusiform bacteria that dominate the rodent intestinal tract, resulting in reduced levels of acetic, propionic, and butyric acids. Cecectomized mice are likely to be a useful model for study of the interaction between intestinal pathogens and the normal microbiota and for studies of translocation of bacteria into host tissues after loss of colonization resistance.

Animals↗
PubMed1987

Development of coronary artery disease in cardiac transplant patients receiving immunosuppressive therapy with cyclosporine and prednisone.

Coronary artery disease (CAD) has been shown in previous uncontrolled studies to be a limiting factor to long-term survival in patients undergoing cardiac transplantation and who were taking conventional immunosuppressive agents. To study the development of CAD after cardiac transplantation in patients taking the newer immunosuppressive agent cyclosporine, we prospectively performed yearly coronary arteriography on all eligible transplantation patients (first year, 57 patients; second year, 30 patients; third year, 14 patients). The prevalence of CAD by life table analysis was 18% at 1 year, 27% at 2 years, and 44% at 3 years. The occurrence of two or more major rejection episodes was associated (p less than .005) with the development of CAD. In two patients who died of CAD, coronary artery histology revealed subintimal inflammatory cellular infiltration in some lesions. These data demonstrate that the prevalence of CAD rises progressively over time and immunologic factors may be important in its development.

Adult↗