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Biomedical subjects

A Lapin

Publications and source records attributed to A Lapin.

32 records · Page 2Linked to original sources
PubMed1989

[Proteinuria in normal pregnancy and in EPH gestosis].

EPH-gestosis (pre-eclampsia-eclampsia) characterized by edema, proteinuria and hypertension occurs primarily in the nullipara, usually after the 20th gestational week. As in normal pregnancy there is striking change in both renal blood flow and glomerular filtration rate a slight increase in urinary protein secretion is not considered abnormal until it exceeds 300 mg/day. Abnormal proteinuria commonly accompanies pre-eclampsia and may be minimal, moderate or severe (even exceeding greater than 25 g/l). Proteinuria was typed mainly of nonselective glomerular origin by using the SDS-disc-electrophoresis. Additionally the clearance ratio of IgG to transferrin in all patients with abnormal proteinuria was evaluated. In none of the patients studied the ratio was less than 0.1 (highly selective). As severe proteinuria is associated with fetal growth retardation, preterm deliveries and prenatal mortality the quantitation and typing of early proteinuria is essential for considering patients who are at risk for developing EPH-gestosis.

Adolescent↗
PubMed1987

[Epidemiology of hypercholesterolemia in the Vienna area].

Hypercholesterolaemia is the most important risk factor in coronary heart disease (CHD). Epidemiological data are not available for the region of Vienna and the aim of this investigation was to obtain them. The municipal health investigation centres are attended by 6000 to 8000 people from the region of Vienna each year, who may voluntarily use these services once a year without any cost. We analysed total cholesterol, HDL cholesterol and the ratio of total to HDL cholesterol of 733 unselected men and women aged 20 to 79 years, who visited one of the centres in May 1986. The classification was carried out according to the recommendations of the National Institutes of Health (Bethesda, USA, 1984). Using these guidelines 26.0% of the men and 26.6% of the women have normal total cholesterol levels. With regard to total cholesterol 55.0% men and 44.3% women have elevated levels and 19.0% of men and 29.2% of women are at high risk of developing CHD. The mean ratios of total to HDL cholesterol, which is closely related to CHD risk, and the proportion with a value above 4.5, which is associated with elevated CHD risk, are closely related to Austrian trends in ischaemic heart disease, which are unfavourable, especially in middle aged men, the so-called "young myocardial infarctions".

Adult↗
PubMed1987

[Gamma 1 heavy chain disease with immune vasculitis and rheumatoid arthritis].

We are reporting about a case of gamma heavy-chain disease (Franklin's disease) with immunovasculitis and rheumatoid arthritis. The diagnosis was confirmed by the results of immunoelectrophoresis of the patient's serum and also by evidence of stimulated lymphocytes without light chain, but having gamma heavy-chain surface proteins. The immunofixation of the serum showed two protein bands of gamma heavy-chains with different loads. These results are confirmed by a two dimensional electrophoresis and isoelectric focussing of the serum proteins. The pathologic protein consists of at least two different heavy-chain proteins (mol wt 40,000 and 80,000) with isoelectric points between pH 5.5 and 7.3. In the urine of the patient pathological gamma heavy chain-protein was found only in a very low concentration. The predominant clinical symptom of the patient was a necrotizing vasculitis which became a therapeutical problem. In the immunofluorescence examination of the skin biopsy specimens, immunoglobulins and C3-complement could be detected in the stratum papillare. This fact would be compatible with the development of antibodies or immune complexes against deposited heavy-chain proteins. The arthropathy and the positive rheumatoid factor could similarly be explained by an immune complex mechanism.

Antigen-Antibody Complex↗
PubMed1986

Monitoring of urinary proteins by SDS electrophoresis in kidney transplant patients.

Sodium dodecyl sulphate (SDS) electrophoresis of urinary proteins was used routinely for monitoring more than 80 kidney transplant recipients as out-patients during one year. Special attention was paid to the question of whether this method can help the clinician to differentiate between a graft rejection reaction and Cyclosporin A-induced nephrotoxic damage. Two cases are presented showing the time course of proteinuric patterns together with serum creatinine, total urinary protein and, in one case, the blood level of Cyclosporin A. Changes of proteinuric patterns signalled the start of rejection and/or nephrotoxicity. A homogeneous collective of 33 kidney recipients (first transplantation) with chronic glomerulonephritis as a basic disease was specially selected. In this collective, the distribution of proteinuric patterns among patients and its dependence on immunosuppressive therapy was investigated. During the observation period of 7 months, two proteinuric patterns, "tubular" and "mixed weak", were found exclusively in Cyclosporin A-treated patients. We ascribe this finding to nephrotoxic effect of Cyclosporin A. We found the SDS electrophoresis of urinary proteins to be a useful, non-invasive method for monitoring Cyclosporin A-treated kidney transplant patients.

Adolescent↗
PubMed1985

A simple routine method for SDS-electrophoresis of urinary proteins in kidney transplant patients.

The molecular weight analysis of urinary proteins can provide useful diagnostic information. For this purpose a routine method of sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-electrophoresis) is described. The main problem in the introduction of this method into the clinical laboratory lies in the availability of "ready-to-use" electrophoresis gels with good and reproducible quality. A device for gel production is therefore described, which is easily constructed from plastic package materials. Polyacrylamide gels are made batch-wise and in advance. They are suitable for various types and techniques of horizontal electrophoresis. Further, a method for analysis of urinary proteins is described, which permits the simultaneous analysis of 22 unconcentrated urine samples within a 3-hour-electrophoresis run. A specially optimized Coomassie blue staining method (overnight) ensures detection of proteins in the concentration range of milligrams per litre. Electrophoretic analyses were documented by photocopying the finished electrophoresis gels.

Electrophoresis, Polyacrylamide Gel↗
PubMed1985

[Ultrasound diagnosis of branchiogenic neck cysts].

16 operatively and histologically proven branchiogenic cysts were evaluated sonographically and the contents of some of them were analysed by microscopy and chemistry. The echographic appearance of all cysts depended on the frequency of the scanheads and ranged from cystic (3.0 MHz) via semi-solid (5.0 MHz) to solid (7.5 MHz). This frequency-related sonographic pattern is caused by the high amount of cholesterin contained in these cysts and permits diagnosis of a branchiogenic cyst.

Adolescent↗
PubMed1984

Two dimensional electrophoresis method for additional characterization of paraproteins in serum. Clinical application of two dimensional electrophoresis, I.

High resolution two dimensional electrophoresis, one of the most potent methods for the analytical separation of proteins, is still used only in specialized laboratories. The present paper describes a two dimensional electrophoresis method, which permits characterization of abnormal serum proteins (paraproteins) and is suitable for application in a clinical laboratory. Both steps of the electrophoresis are performed by the "flat bed technique". The critical transfer from the isoelectric focusing step to the gel of the second dimension is facilitated by use of specially prepared foil stencils. The practicality of the procedure was considerably increased by using commercially available materials such as gels for isoelectric focusing and the silver stain kit. The analysis can be calibrated by measurement of the pH gradient and by using molecular weight protein standards. Moreover, two different samples can be analysed simultaneously in the same gels of the first and the second dimension. This permits a direct comparison of the sample of interest with the sample of reference. Serum samples showing an abnormal band ("M gradient") in routine methods such as agarose gel electrophoresis, were analysed by the described method. Some "M gradients" consisted of single abnormal proteins. Another sample showed a "M gradient" composed of a number of abnormal protein spots in our method. Questions concerning pathobiological and clinical evaluation of the findings justify further studies using this method.

Electrophoresis, Agar Gel↗
PubMed1980

Simple and rapid mass fragmentographic method for the determination of glycine in brain tissue.

A method for the sensitive and selective determination of glycine in brain tissue has been developed. Small samples of brain tissue were homogenized by sonication in 0.7 N formic acid, and [1,2 13C2,15N]glycine was then added as internal standard. After centrifugation, aliquots of the supernatants were dried and the resulting residues were derivatized in a single step with heptafluorobutyric anhydride and hexafluoroisopropanol. After removal of the derivatization reagents by evaporation the residues were dissolved in ethyl acetate and an aliquot was analyzed by mass fragmentography. Quantification was performed by comparing the ratio of peak areas of glycine and its internal standard.

Animals↗
PubMed1980

A sensitive and specific method for the determination of chlorimipramine and desmethylchlorimipramine in plasma using selected ion monitoring with chemical ionization.

Chlorimipramine and other tricyclic antidepressant drugs are widely used for the treatment of depressive disorders. We analysed chlorimipramine and its major metabolite desmethylchlorimipramine after a two step extraction from plasma with diethylether. The underivatized samples were separated on a short (40 cm x 2 mm) silanized glass column packed with 3% OV 17 on Chromosorb HPW-DMCS, 80-100 mesh, which was directly coupled to the mass spectrometer. Detection of the tricyclic antidepressant drugs and their respective deuterium labelled internal standards was performed by chemical ionization using methanol vapour as reactant gas. The method is simple and sufficiently sensitive to permit the routine analysis of therapeutic plasma levels of tricyclic antidepressant drugs in extracts originating from 1 ml plasma.

Antidepressive Agents, Tricyclic↗
PubMedDate not supplied

Diagnostic use of an analysis of urinary proteins by a practicable sodium dodecyl sulfate-electrophoresis method and rapid two-dimensional electrophoresis.

Two methods suitable for routine clinical analyses of urinary proteins are presented and compared. The first is a horizontal sodium dodecyl sulfate-polyacrylamide gel electrophoresis technique, suitable for simultaneous analysis of 20 native urinary samples. This method uses polyacrylamide gradient gels, prepared with a laboratory-built gel casting device. The second method is a rapid two-dimensional electrophoresis procedure, combining cellulose acetate electrophoresis and sodium dodecyl sulfate-electrophoresis. The first step uses a routine system (Chemetron), the second separation step followed by staining with Coomassie Brilliant Blue R is performed on the PhastSystem. The resulting two-dimensional patterns reveal urinary proteins distributed according to the 5-zone pattern of native proteins (albumin, alpha-1, alpha-2, beta, gamma-globulin) as well as to the logarithm of their molecular weights. Examples of (routine) diagnoses with a special interest in the monitoring of kidney transplant patients are shown.

Cellulose↗
PubMedDate not supplied

A practicable two-dimensional electrophoresis of urinary proteins as a useful tool in medical diagnosis.

Practical experience with a rapid two-dimensional electrophoresis technique for routine analysis of urinary proteins is discussed. The method consists of cellulose acetate electrophoresis in combination with sodium dodecyl sulfate (SDS) electrophoresis, performed together with Coomassie Blue gel staining on "PhastSystem". Over 400 analyses were performed within the time of two years. Most patients were from nephrological, urological and kidney-transplant departments. Some of them were from obstetric, pediatric or oncological departments. A systematic discussion and evaluation of the two-dimensional protein pattern with typical examples is outlined.

Blood Proteins↗