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Biomedical subjects

A L Barsoum

Publications and source records attributed to A L Barsoum.

25 records · Page 2Linked to original sources
PubMed1977

Serum immunoglobulin E level and search for myelin basic protein specific IgE antibodies in patients with multiple sclerosis.

Circulating levels of immunoglobulin E (IgE) have been determined by solid-phase radioimmunoassay in groups of patients with different clinical courses of multiple sclerosis (MS) and in healthy controls. The geometric mean of the serum IgE level in the MS patients was somewhat less than that in the control group (64 U/ml), but this decrease was not significant. Contrary to the findings of Campbell [1, 2, 3, 4], circulating IgE in the MS patients was not specifically adsorbed on bovine myelin basic protein (BMBP) immunoadsorbent and does not give a positive immunofluorescence using guinea pig brain sections.

Adult↗
PubMed1977

Circulating IgE levels in a normal human population.

Circulating IgE levels were measured in a non-selected normal population using a solid phase radioimmunoassay (Phadebas Radioimmunosorbenttest "RIST"). The population was divided according to age into 6 subpopulations. Both the main and the subpopulation showed multimodal distributions with 3 maxima of their IgE levels and not a Gaussian distribution. According to the maxima, the multimodal distribution was divided into 3 groups designated AA, Aa and aa. The IgE geometric mean in each group differs statistically significant (p less than 0.01). The proportions of individuals in each group agreed with the theoretical frequencies derived from the Hardy-Weinberg law and with frequencies for atopic (a) and non-atopic (A) alleles (0.152 and 0.846, respectively), as calculated from clinical studies by WIENER et al. (1). After exclusion of Aa and aa individuals from the population the distribution was shown to be normal. The IgE geometric mean in the "net" main population was found to be 33 U/ml. This value is slightly higher than those of recent studies on IgE levels. The mean level of IgE in the different age-groups did not vary significantly from that of the main population although a tendency for a decrease of IgE levels with increasing age was noticeable.

Adolescent↗
PubMed1974

Isolation and partial characterization of macromolecular urinary aggregates containing carcinoembryonic antigen-like activity.

Carcinoembryonic antigen-like substances in the urine of patients with bladder carcinoma and of healthy male subjects occur in a wide range of average molecular sizes. Some of these substances are glycoproteins giving reactions of antigenic identity with carcinoembryonic antigen (CEA) derived from colorectal carcinoma and occur in aggregation with proteins showing antigen specificities of albumin, haptoglobin and the heavy chains of immunoglobulins G, A and M. Recoveries of CEA-like activities, following Sepharose 4B chromatography of urinary materials of molecular size > 3 × 10(4), varied from 40 to 1530%. Treatment with 1 mol/1 HClO(4) caused an apparent solubilization of 85% of the CEA-like activity of such materials from the urine of a patient with bladder carcinoma and raised the specific CEA-like activity of the solubilized product to 379% of that of the untreated materials.

Albumins↗
PubMed1974

Carcinoembryonic antigen-like substances of human urothelial carcinomas. Isolation of components from pathological urine and comparison with colorectal carcinoma antigens.

Urines of several patients with urothelial carcinomas contain inhibitors of the immunoreaction between carcinoembryonic antigen derived from human colorectal carcinomas and monospecific goat antiserum raised against the antigen. These inhibitors range in approximate molecular weights from less than 1000 to several millions, and two have been isolated by a combination of extraction, gel filtration and electrophoretic procedures. These are respectively a macromolecular aggregate, component UCEA-3, which is excluded by Sepharose 4B, and a glycoprotein(s) component, UCEA-1, with mean molecular weight (2x10(5)) similar to that of carcinoembryonic antigen. Comparison of the properties of component UCEA-1 and carcinoembryonic antigen on gel filtration, electrophoresis, immunoelectrophoresis and density gradient ultracentrifugation indicates that these substances of similar molecular size and net charge differ in some immunochemical properties.

Animals↗
PubMedDate not supplied

37 kiloDalton oncofetal antigen protein and immature laminin receptor protein are identical, universal T-cell inducing immunogens on primary rodent and human cancers.

Based upon positive immunologic comparisons, protein and cDNA sequencing, and in vitro and in vivo immunogenicity studies we propose that carcinomas, sarcomas and lymphomas/leukemias of rodents and humans share 37 kDa Onco-Fetal Antigen [OFA] as a T and B-lymphocyte stimulating, universal tumor specific transplantation antigen [UTSTA]. In the past four years, biochemical studies from several laboratories studying laminin receptor protein and immunological studies of OFA from our laboratories independently converged. OFA protein and immature or precursor Laminin Receptor Protein [iLRP] are > 99% identical proteins based on amino acid and cDNA sequencing and immunobiology studies summarized here. Acquired expression of 37 kDa OFA/iLRP enables malignant tumor cells to penetrate laminin tissue and vessel barriers. 37 kDa OFA/iLRP activates precursor thymic anti-OFA/iLRP specific cytotoxic T cell which kill emerging pretumor cells. Our reported findings also demonstrate that OFA/iLRP can function to induce specific immunoregulatory CD8 T-suppressor cells secreting IL-10 which impair effector T-cell killing of emerging tumor cells non-specifically and thereby facilitate tumor progression. Potential applications of OFA/iLRP detection in early cancer formation, for monitoring patient T cell subclass responses to OFA/iLRP as a predictor of tumor progression, and the use of OFA/iLRP peptides for specific anti-tumor immunotherapy are presented.

Amino Acid Sequence↗