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Biomedical subjects

A Kuroda

Publications and source records attributed to A Kuroda.

At least 145 records · Page 8Linked to original sources
PubMed1983

Retrostatin, a new specific enzyme inhibitor against avian myeloblastosis virus reverse transcriptase.

A novel enzyme inhibitor against RNA-directed DNA polymerase of avian myeloblastosis virus was produced by an isolate of a new streptomycete for which the name Streptomyces retrostaticus is proposed. This enzyme inhibitor, which was named retrostatin, did not inhibit DNA-directed DNA polymerase of Escherichia coli and DNA-directed RNA polymerase of Ehrlich ascites tumor cells. Retrostatin was produced by the microorganism together with streptonigrin. These two substances were extracted from the culture broth with ethyl acetate at acidic pH. Retrostatin is an acidic pH indicator and the free acid was recovered as a red powder. Retrostatin had weak antibiotic activities against Gram-positive bacteria and yeasts.

Animals↗
PubMed1982

Protection of pigs against mosquito-borne Japanese encephalitis virus by immunization with a live attenuated vaccine.

Pigs were vaccinated with an attenuated Japanese encephalitis virus (JEV) vaccine and challenged with virulent JEV, either by subcutaneous injection or by exposure to infected mosquitoes. The vaccinated pigs developed circulating antibodies to JEV. After challenge they did not develop viremia detectable by inoculation of their serum in suckling mice. They were also unable to transmit virus to mosquitoes fed on their skin. In contrast, unvaccinated pigs, whether challenged by injection or by mosquito bites, developed viremia and did transmit virus to mosquitoes which were allowed to bite them. Transmission seemed possible for only 3 days post-infection.

Animals↗
PubMed1981

[Analysis of RIA standard curve by log-logistic and cubic log-logit models (author's transl)].

In order to improve goodness-of-fit in RIA standard analysis, programs for computing log-logistic and cubic log-logit were written in BASIC using personal computer P-6060 (Olivetti). Iterative least square method of Taylor series was applied for non-linear estimation of logistic and log-logistic. Here "log-logistic" represents Y = (a--d)/(1+log(X)/c)b)+d As weights either 1, 1/var(Y) or 1/sigma 2 were used in logistic or log-logistic and either Y2(1--Y)2, Y2(1-Y)2/var(Y), or Y2(1--Y)2/sigma 2 were used in logistic or log-logistic and either Y2(1--Y)2, Y2(1--Y)2/var(Y), or Y2(1--Y)2/sigma 2 were used in quadratic or cubic log-logit. The term var(Y) represents squares of pure error and sigma 2 represents estimated variance calculated using a following equation log(sigma 2 + 1) = log(A)+J log(y). As indicators for goodness-of-fit, MSL/Se2, CMD% and WRV (see text) were used. Better regression was obtained in case of alpha-fetoprotein by log-logistic than by logistic. Cortisol standard curve was much better fitted with cubic log-logit than quadratic log-logit. Predicted precision of AFP standard curve was below 5% in log-logistic instead of 8% in logistic analysis. Predicted precision obtained using cubic log-logit was about five times lower than that with quadratic log-logit. Importance of selecting good models in RIA data processing was stressed in conjunction with intrinsic precision of radioimmunoassay system indicated by predicted precision.

Computers↗
PubMed1980

Computed tomography and ultrasound in the diagnosis of intrahepatic calculi.

Computed tomography (CT) and ultrasound were used to evaluate intrahepatic biliary calculi in 16 patients. Thirteen demonstrated high-density shadows in dilated intrahepatic ducts on CT. Of the 11 who also underwent ultrasonography, 7 had positive findings. Strong echoes with acoustic shadows in dilated intrahepatic biliary ducts are diagnostic of stones. CT and ultrasound are useful in evaluating intrahepatic calculi, with CT being particularly helpful in postoperative cases.

Adult↗
PubMed1978

Epidemiological studies on Japanese encephalitis in Kyoto City area, Japan. III. Seasonal prevalence of virus infections in several pig populations shown by virus recovery from engorged Culex tritaeniorhynchus summorosus.

When vector mosquitoes engorged by feeding on pigs are tested for virus recovery after incubation for 7 to 10 days, the results may show mosquito infection itself. Therefore, seasonal prevalence of infection in each pig population can be estimated from course of the infection rate among mosquitoes. Many mosquitoes of the main vector of Japanese encephalitis virus, Culex tritaeniorhynchus summorosus, collected by light traps everyday or every other day in some pig sheds from 1967 to 1970 were tested for virus recovery after incubation. The tests were positive during about a month period each summer, and the peak infection rate was high being over 10%. The course of the mosquito infection showed a certain pattern with one or two peaks between the initial recovery and the highest peak. From the interval of 12 to 13 days after the first recovery to the peak, cyclic infection between the pig and the mosquito may occur at the same interval.

Animals↗
PubMed1978

Epidemiological studies on Japanese encephalitis in Kyoto City area, Japan. IV. Natural infection in sentinel pigs.

Natural infection with Japanese encephalitis virus in three sentinel pigs held in separate experimental huts was examined daily by virus recovery from blood samples of the pigs and from mosquitoes after incubation for about 7 days from their blood feeding and by HI antibody titration of the blood samples. After a period of low infection rates, below 6%, for about two weeks in engorged Culex tritaeniorhynchus summorosus, high mosquito infections of over 30% from each viremic pig occurred for two to three days. The pigs may be probably have been bitten by many infected but not infective mosquitoes in a period of about 10 days before infection.

Animals↗
PubMed1978

Epidemiological studies on Japanese encephalitis in Kyoto City area, Japan. II. Annual patterns of virus dissemination on virus recoveries from unfed Culex tritaeniorhynchus summorosus.

Annual patterns of dissemination of Japanese encephalitis virus in vector mosquitoes have been investigated at the main collection station from 1965 through 1973 at some other stations from 1969 through 1971. The virus was recovered usually from Culex tritaeniorhynchus summorosus during a period of about a month from July to August every year till 1969, and from August to September after 1970, although at some of the stations the virus was recovered intermittently for longer or shorter terms. Higher infection rates were recorded with the mosquitoes caught at the stations near to pig sheds than at the stations far from pig sheds. The infection rates at the peak of virus recovery in high epidemic year (1965 to 1967) were higher, being over 2%, than those in lower or latent epidemic years (1968 to 1973). Human patients of Japanese encephalitis were found in 17 to 20 days after the appearance of the highest peak of the infection rate in mosquitoes.

Animals↗