Biomedical subjects
A Kuramoto
Publications and source records attributed to A Kuramoto.
[Studies on platelet derived leukocyte activating substances. (1). Platelet derived granulocyte aggregating factor].
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Induction of ornithine decarboxylase by treatment with phospholipase C.
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Induction of ornithine decarboxylase in guinea-pig lymphocytes and its relation to phospholipid metabolism.
Treatment of lymphocytes with exogenous phospholipase C (phosphatidylcholine cholinephosphohydrolase, EC 3.1.4.3.) derived from Clostridium perfringens at concentrations similar to those which induced ornithine decarboxylase (L-ornithine carboxy-lyase, EC 4.1.1.17) activity produced diacylglycerol and phosphatidate. A divalent cation ionophore, A23187, and phytohemagglutinin induced not only diacylglycerol formation, but also ornithine decarboxylase activity. Dibutyryl cAMP inhibited both diacylglycerol formation and ornithine decarboxylase induction to a similar extent in phytohemagglutinin-stimulated lymphocytes, but stimulated them somewhat in ionophore A23187-activated lymphocytes. This suggests that the activation of intracellular phospholipase C and the formation of diacylglycerol is involved in ornithine decarboxylase induction in lymphocytes.
Lymphocyte subpopulations in pernicious anemia.
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Rhabdomyosarcoma of the maxillary sinus. A case report.
A rare case of adult rhabdomyosarcoma originating from the left maxillary sinus is reported. A 22-year-old Japanese woman was referred to the Department of Otolaryngology, Hiroshima University, for investigation of the primary source of a metastatic tumor in the bone marrow. An X-ray of the sinuses revealed a tumor in the left maxillary sinus with extension into the nasal cavity, ethmoidal and sphenoidal sinuses. The patient was managed with a chemotherapeutic regimen consisting of high-dose methotrexate, adriamycin, vincristine, cyclophosphamide and prednisolone. After three courses of this chemotherapy, the tumor had decreased in size by about 90 per cent, as indicated by X-rays. The remaining tumor was removed by an extended Denker's operation. Following the surgery another seven courses of chemotherapy were given and during the eight months following the operation the patient has been free from any local recurrence.
Effect of diethyldithiocarbamate on platelet aggregation.
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[Cytogenetic studies on patients with leukemia and RAEB found in atomic bomb survivors].
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[Immunosuppression or androgen therapy for aplastic anemia: a cooperative study].
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[A study on platelet Factor XI-like activity in a family with congenital Factor XI deficiency].
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[Four gastrectomized cases associated with chronic leukemia].
We reviewed 4 gastrectomized cases associated with chronic leukemia. Three patients had chronic myelogenous leukemia, one had hairy cell leukemia. Subtotal gastrectomy was performed in all cases; two operations were for gastric cancer and two for bleeding gastric ulcers. The spleen was removed in three patients without any trouble. There were no difficulties in managing the patients during and after surgery and their leukemia was not aggravated during these periods.
[Double minute chromosomes (DMs) in a case of acute myelocytic leukemia (M2: FAB classification)].
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[Cytogenetic studies on chronic myelocytic leukemia. Evolution of clones with additional Ph1 chromosome detected by a large number of karyotype analysis].
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Defective ultraviolet-induced DNA repair in bone marrow cells and peripheral lymphocytes of patients with refractory anemia with excess of blasts.
The extent of ultraviolet (UV) irradiation-induced DNA repair was measured in bone marrow cells and peripheral lymphocytes of patients with refractory anemia with excess of blasts (RAEB). Bone marrow cells from RAEB, when exposed to a 2 J/m dose of UV, exhibited 50% lower incorporation of tritiated thymidine than those of control subjects. A similar finding was observed in the peripheral lymphocytes. These data suggest that bone marrow cells and peripheral lymphocytes from RAEB are deficient in repair of UV-induced lesions by DNA. Furthermore, this impaired DNA repair efficiency in RAEB was not related to the presence or absence of a karyotype abnormality.
[Effect of antithrombin III concentrate infusion therapy on congenital antithrombin III deficiency].
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Hexosaminidase isoenzyme profiles in leukemic cells.
The isoenzyme profiles of hexosaminidase in leukemic cells from 39 patients were examined with DEAE-Sephadex chromatography. There was a clear difference in the isoenzyme composition between normal lymphocytes and granulocytes. In acute non-T/non-B lymphocytic leukemia (ALL) a characteristic alteration was found in the intermediate forms of hexosaminidase (Hex I), which was significantly higher than those of normal lymphocytes (p less than 0.001). Subtypes of Hex I (Hex i1-i4) and the heterogeneity of their abnormal expression in ALL was demonstrated. There was no type-specific alteration of the isoenzyme profiles in T-cell leukemia. Leukemic cells of acute myelocytic leukemia (AML) had significantly higher Hex P component than granulocytes (p less than 0.01) and ALL cells (p less than 0.01). The increase of Hex P was evident in childhood AML. There was a significant difference in Hex P level between childhood AML and adult myelo(mono)genous leukemia (p less than 0.001). Chronic myelo(mono)genous leukemia showed similar isoenzyme compositions to normal granulocytes. The isoenzyme profiles in acute undifferentiated leukemia differed from those in other types of leukemia. Isoenzyme analysis might be useful for probing the nature and the intrinsic biochemical abnormality of leukemic cells.
Induction of ornithine decarboxylase by treatment of guinea pig lymphocytes with phospholipase C.
Treatment of guinea pig lymphocytes with Clostridium perfringens phospholipase C but not with Naja naja snake venom phospholipase A2 increased ornithine decarboxylase activity. The increase in ornithine decarboxylase activity was suppressed by actinomycin D or cycloheximide, suggesting that de novo syntheses of RNA and protein are necessary for the increase in the enzyme activity. These results suggest that the activation of phospholipase C rather than that of phospholipase A2 is responsible for induction of ornithine decarboxylase during lymphocyte transformation.
Two cases of phosphofructokinase deficiency associated with congenital hemolytic anemia found in Japan.
Two kindreds of phosphofructokinase (PFK) deficiency associated with congenital nonspherocytic hemolytic anemia and mild myopathy were found in Japan. Both probands had jaundice, gallstones, and slight to moderate degree of exercise intolerance. They showed decreased level of red cell PFK activity and no increase of blood lactate in forearm ischemic exercise test. We studied these probands' red cell PFKs by partial purification and condensation. Muscle type isozyme of PFK in both cases was not demonstrable in starch gel electrophoresis and DEAE-Sephadex chromatography. The clinical symptoms are considered to be due to a defect of muscle type isozyme.