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Biomedical subjects

A Krzyszkowska

Publications and source records attributed to A Krzyszkowska.

At least 19 recordsLinked to original sources

Comparative evaluation of the diagnostic possibilities by urography, arteriography, scintigraphy and pharmacophlebography in traumatic lesions of kidney parenchyma in dogs.

Traumatic lesions of kidney parenchyma, not penetrating into the renal collective system, are the most frequent kidney traumas in man. The absence of typical clinical symptoms and the inability of comparing radiological investigations with the actual state are strongly limiting the diagnostic possibilities. The object of the present work has been to give a comparative evaluation of urography, arteriography, scintigraphy and pharmacophlebography in 22 dogs in which lesions of kidney parenchyma were performed operatively. From this work it follows that pharmacophlebography is of the greatest value because it detects traumatic lesions of the parenchyma in 80-100%. This method carries no risk even at an early period of kidney lesion, and does not lead to late changes in the parenchyma and kidney vessels.

Angiography↗

Further studies on proteinase inhibitors separated from human serum by deae sephadex chromatography.

Proteinase inhibitors adsorbed from human serum on DEAE Sephadex A 25 0.25 or 0.3 mol/l NaCl were purified by affinity chromatography on a trypsin-Sepharose 4B column, by gel filtration, and by DEAE cellulose chromatography. Small amounts of TCI-I (desorbed from the ion-exchange between 0.25-0.3 mol/l NaCl), and TCI-II (desorbed at NaCl concentration above 0.3 mole/l) with high specific activity were obtained. The low recovery of inhibitory activity (below 9% was due to a molecular transformation of these inhibitors after contact with the immobilized trypsin. The low-molecular weight derivatives were formed that lost their ability to adsorbe on ion-exchanger at 0.25 or 0.3 mole/l salt concentration.

Chromatography, Affinity↗