Biomedical subjects

A Krstić

Publications and source records attributed to A Krstić.

At least 19 recordsLinked to original sources

PubMed2006

The effect of interleukin-17 on hematopoietic cells and cytokine release in mouse spleen.

To evaluate whether the response of hematopoietic cells to interleukin-17 (IL-17) depends on the tissue microenvironment in which hematopoiesis occurs, the influence of recombinant mouse IL-17 on spleen hematopoietic cells and cytokine release was assessed in normal mice in vitro and in vivo. In vitro, IL-17 did not significantly affect the growth of granulocyte-macrophage (CFU-GM) and erythroid (BFU-E and CFU-E) derived colonies. A single injection of IL-17 in vivo exhibited stimulatory effects on hematopoietic cells from both granulocytic and erythroid lineages. The increased number of metamyelocytes 48 h after treatment imply to the IL-17-induced stimulation of granulopoiesis. The number of BFU-E was increased at 24 h, while the number of CFU-E increased 6 h and 24 h after treatment. Since the same treatment in the bone marrow decreased the number of CFU-E, it may be concluded that the local microenvironment plays an important role in IL-17-mediated effects on CFU-E. IL-17 increased the release of IL-6 both in vitro and in vivo, but showed tendency to suppress the constitutive secretion of IL-10 by spleen cells. Our results suggest the complexity of target cell response and interplay of secondary induced cytokines by IL-17 in different hematopoietic organs.

Animals↗

PubMed2004

In vivo effects of interleukin-17 on haematopoietic cells and cytokine release in normal mice.

In order to gain more insight into mechanisms operating on the haematopoietic activity of the T-cell-derived cytokine, interleukin-17 (IL-17) and target cells that first respond to its action in vivo, the influence of a single intravenous injection of recombinant mouse IL-17 on bone marrow progenitors, further morphologically recognizable cells and peripheral blood cells was assessed in normal mice up to 72 h after treatment. Simultaneously, the release of IL-6, IL-10, IGF-I, IFN-gamma and NO by bone marrow cells was determined. Results showed that, in bone marrow, IL-17 did not affect granulocyte-macrophage (CFU-GM) progenitors, but induced a persistant increase in the number of morphologically recognizable proliferative granulocytes (PG) up to 48 h after treatment. The number of immature erythroid (BFU-E) progenitors was increased at 48 h, while the number of mature erythroid (CFU-E) progenitors was decreased up to 48 h. In peripheral blood, white blood cells were increased 6 h after treatment, mainly because of the increase in the number of lymphocytes. IL-17 also increased IL-6 release and NO production 6 h after administration. Additional in vitro assessment on bone marrow highly enriched Lin- progenitor cells, demonstrated a slightly enhancing effect of IL-17 on CFU-GM and no influence on BFU-E, suggesting the importance of bone marrow accessory cells and secondary induced cytokines for IL-17 mediated effects on progenitor cells. Taken together, these results demonstrate that in vivo IL-17 affects both granulocytic and erythroid lineages, with more mature haematopoietic progenitors responding first to its action. The opposite effects exerted on PG and CFU-E found at the same time indicate that IL-17, as a component of a regulatory network, is able to intervene in mechanisms that shift haematopoiesis from the erythroid to the granulocytic lineage.

Animals↗

PubMed1996

[Multicystic dysplastic kidney. Therapeutic dilemmas and personal experience].

A multicystic dysplastic kidney (MCDK) is one of the most frequent causes of abdominal mass in the neonate. Prenatal echography permits early and frequent diagnostics. It is a nonfamilial disease without associated cystic disease of the pancreas, liver or lungs. Indications for elective surgery are clear when there is a symptomatic disease. However, treatment of asymptomatic patients is controversial. A rising number of authors prefer nonsurgical approach, leaving MCDK intact with a close follow-up of patients for possible severe complications (malignancy, hypertension, infection, pain, rupture). We treated 15 patients with MCDK from 1984 to 1994. Diagnosis was passed antenatally in 7 (47%) patients, accidentally in 2, and based on the presence of abdominal mass in 6 patients. Two patients had renal failure due to the abnormal contralateral kidney. Nine patients were operated on and 6 were treated nonsurgically. The risk of complications associated with nonsurgical treatment, easiness of efficient surgery at the age of 3-6 months, avoiding stress in the child and family due to long-term follow-up, all suggest operative treatment. We suggest to parents both operative and nonoperative options, explaining the risk and danger of both. We believe that nephrectomy is the best solution in a child with MCDK who is growing, develops hypertension, with uncertain diagnosis or when adequate follow-up is impossible.

Female↗

PubMed1993

[Skin changes in patients with Lyme borreliosis].

In introduction some clinical characteristics of Erythema migrans, Borrelia lymphocytoma and acrodermatitis chronica atrophica has been described. The importance of atypical forms of Erythema migrans and the difficulties in differential diagnosis of cutaneous manifestation has been stressed. In a prospective, and partly retrospective investigation of 1292 persons with tick bites, signs of Lyme borreliosis have been found in 18.96%. Number of such persons seen in dermatology wards is rising, and 18.2% of these are children less than 15 years of age. Patients seen in dermatology are mostly women (56.5%:43.5%). Nearly half of the patients with Erythema migrans did not known that they had a tick bite (42.5%). Lyme borreliosis was manifested mainly as Erythema migrans, 89% of patients. Borrelia lymphocytoma was encountered in 2%, and Acrodermatitis chronica atrophicans in 0.4% of patients, significantly less than in other reports. Sclerotic skin lesions were found in 4.1% of patients, and some macular and urticarial lesions were recorded. An incubation period generally less than three weeks preceded to skin manifestations, but in some patients this period could not be recorded. Skin lesions were located on lower extremities in 50.4% of patients, trunk in 25.5%, and upper extremities in 10.5% of patients. In 87% of patients skin lesions lasted less than three weeks. Symptoms were present in 62% of patients. Seropositivity to Borrelia burgdorferi has been found in 10.2% of patients, mostly three weeks after the tick bite.

Adolescent↗

PubMed1990

[Minor malformation score in congenital cleft of the lip and palate].

The authors analyzed minor malformation score in 63 children (36 boys and 27 girls) with congenital cleft of the lip and palate in order to evaluate purposefulness of its application as a screening method in detecting various major malformations. The results obtained revealed extremely increased minor malformation score in our patients and confirmed that an increased minor malformation score is associated with major malformation, i.e. clefts of the lip and palate. Therefore, it could be used as a screening method for detection of major malformations.

Cleft Lip↗

PubMed1990

[Morbidity and mortality in adolescents in Vojvodina].

Adolescents. The aim of this study was to analyze the morbidity and mortality of adolescents in SAP Vojvodina. For the analysis, data was used from the statistical yearbooks on the health protection of the population and data from individual statistical reports. The period from 1980 to 1986 was observed. In order to more easily follow the morbidity and mortality, and for a better insight into the pathology, the adolescents were divided into 2 age groups; the younger one from 10 to 14 years of age, and the older one from 15 to 19. Adolescents in Vojvodina mostly ail from respiratory diseases, illnesses of the digestive tract, and infective diseases, but a significant role in the morbidity is also taken by injuries and poisoning. Adolescents of the age group from 10 to 14 years, get sick more often. In SAP Vojvodina 156 adolescents die annually. The highest mortality is due to injuries and poisoning (16.36/1,000,000), and after that comes the mortality due to neoplasms (5.84/1,000,000), and the diseases of the respiratory and circulatory system (3.5/1,000,000). Mortality is higher in the adolescent group from 15 to 19 years of age. With this study only a partial insight was achieved into the pathology of the adolescent age group. In order to attain a full insight, it is absolutely necessary to actively and prospectively follow the health state of adolescents and the conditions of environment.

Adolescent↗

PubMed1989

[Hereditary and congenital defects in children in the regions of Indija-Stara Pazova and Pancevo-Kovin].

An analysis of frequency and range of hereditary and congenital defects was performed in all infants born in the period from January 1 to December 31, 1986 in the Ward for Neonates at the Department of Gynecology and Obstetrics in Panĉevo and in the out-patient maternity home in Indija and Kovin. In Panĉevo 2.559 (1.286 m. and 1.273 f.) newborn infants were born, i.e. there were 2.314 (1.178 m. and 1.136 f.) mature children and 245 (108 m. and 187 f.) premature children. In Indija 367 (180 m. and 187 f.) newborn infants were born, i.e. there were 355 (174 m. and 181 f.) mature children and 12 (6 m. and 6 f.) premature children. In Kovin 146 (65 m. and 81 f.) newborn infants were born, i.e. 150 (82 m. and 68 f.) mature and 10 (1 m. and 9 f.) premature children. A total 3.072 (1.531 m. and 1.541 f.) newborn infants in all three places were born. In all three places there was a total of 2.805 (1.417 m. and 1.387 f.) mature and 267 (115 m. and 152 f.) premature children. In Panĉevo 68 children were born with defects (40 m. and 28 f.), i.e. 2.66% (3.11% m. and 2.20% f.). In Indija only one defect was noted in one female child (0.53% related to female newborns).(ABSTRACT TRUNCATED AT 250 WORDS)

Congenital Abnormalities↗

PubMed1989

[Epidermolysis bullosa hereditaria dystrophica: diagnostic problems].

A female patient at the age of 8 years was presented in this paper. The patient was affected by an exceptionally rare disease. A complete clinical picture of disease together with its complication on the esophagus was set forth. According to the literature available a contemporary classification of disease was made and therefore, this case was put into one of the possible varieties of disease.

Child↗

PubMed1988