Light is emitted in systems containing N-chloramines or hypochlorite, H(2)O(2) and N-(4-aminobutyl)-N-ethylisoluminol (ABEI). The emission is enhanced by 4-iodophenol (PIP) in alkaline solution (1 mol/L NaOH), while at lower pH range (9-11) PIP is not only inactive but also its presence reduces chemiluminescence (CL) of the monochloramine-H(2)O(2)-ABEI system to the background. Two procedures for ABEI-labelled IgG assays were developed, with PIP in 1 mol/L NaOH and without PIP at pH 11, and the standard curves of free ABEI in these conditions were examined. We suggest also that the oxidative deamination of taurine chloramine leads to the formation of the various carbonyl derivatives and their formation is accelerated in the presence of H(2)O(2), especially in less alkaline solutions (pH 11). Moreover, the formation of enol forms of aldehydes in assay buffers was observed. The yield of the phenoxy radical mediators of ABEI oxidation and the pH-dependent H(2)O(2):HO(2) ratio seems to be decisive for the overall CL in the system examined. The main advantage of this method is that CL does not need precise timing of measurements and assays can be performed over a long period of time (hours) using a plate luminometer.

Aldehydes↗

PubMedDate not supplied

Effect of 1-anilinonaphthalene-8-sulphonate on phase transition temperature of dipalmitoylphosphatidylcholine liposomes.

The effect of 1-anilinonaphthalene-8-sulfonate (ANS) on the thermotropic phase transition of dipalmitoylphosphatidylcholine (DPPC) bilayers was examined by differential scanning calorimetry. The main phase transition temperature was found to be shifted to lower values in the presence of the probe. The shift strongly depends on pH and the presence of salts. This indicates that the penetration of the probe of the hydrocarbon moiety of the bilayer is influenced by coulombic interactions. Pretransition phenomena are also affected. The implications for the interpretation of experimental data of biomembrane studies are discussed.

Anilino Naphthalenesulfonates↗

PubMedDate not supplied

Questionnaire regarding the knowledge of the rules of organ procurement from cadaveric donors for transplantation directed to the directors of medical institutions in Poland.

We assessed the level of knowledge of organ procurement regulations among the directors of medical institutions in Poland. We also sought to promote the objection form, and the activity of the Central Register of Objections. A questionnaire consisting of 10 questions was sent to 381 random medical health care institution directors countrywide. In 89% of surveyed institutions, the written text of the organ procurement regulations was available and 94% of directors knew the forms of objection, but in 26% of institutions the form was not available and in 14% it was never obtainable. In the medical institutions directors' opinions, the estimated number of objections is 13% of the population in Poland. Organ transplantation is a form of treatment most medical institutions are familiar with, but the matter of donation is not as well known as transplantation, as observed in 48% of questioned institutions.

Cadaver↗

PubMedDate not supplied

Geography of the referred potential liver recipients and donors in Poland in 2004.

Our aim was to assess the accessibility of potential liver recipients to cadaveric organs and the ability of transplant teams to realize recipients needs in Poland in 2004. Our calculations revealed that in Poland the number of cadaveric liver transplants was two to three times lower than in other countries and is insufficient to meet the needs, also the number of referred potential liver recipients is two to three times lower than expected.

Geography↗

PubMedDate not supplied

[ECT versus transcranial magnetic stimulation (TMS): preliminary data of computer modeling].

The essential issue of electroshock therapy (ECT) is the activity of physical stimulus, i.e., the electric current, on the disturbed structures of the brain. ECT sessions--when chronically applied for evoking antidepressive effects--are responsible for the appearance of excessive incitement in the neuronal net in the brain tissue in a form of self-sustaining after-discharge (SSAD) (convulsive attack characteristic for ECT). The study presents the computer research on basic biophysical phenomena of electroshock therapy (flow of electric current in the structures of the head just before convulsive attack). Five-layer 3-D model of the head was created in OPERA-3D (Vector Fields Ltd., Oxford), general 3 dimensional issues solver. Geometrical dimensions and electrophysical properties of each layer correspond with natural properties. The model was subjected to the action of electric stimulation (parameters identical to those applied in clinical conditions). Analysis of the flow in particular layers revealed the crawling/spreading effect present not only in the scalp layer but also in the layer of cerebrospinal fluid. The effect is conditioned by "deeper situated" lesser conduction of electricity-respectively skull bones, brain tissue. Crawling effect is the reason why only 5-15% of the electricity applied on the surface of the head reaches the surface of the brain. Electro-stimulation examinations also showed that the values of the so called density of the current in layers of brain tissue balanced between 1-10 mA/mm2. The current parameters of ECT were effective in evoking subsequent convulsive attack and safe for the brain tissue. The model was subjected to the action of magnetic stimulation according to the parameters of neurologic technique of transcranial magnetic stimulation (TMS). ELECTRA module was used to solve wire-current issues. The examination showed more regular distribution of current vectors in all layers of the head. The density of cerebral cortex was 0.1-1 mA/mm2, confirming markedly lesser current charge than that observed during ECT. The problem of magnetic stimulation efficacy in irritating deep structures of the brain demands further studies.

Electroconvulsive Therapy↗

PubMedDate not supplied

[Progress in the treatment of advanced neuroblastoma].

Explore the source record for details and available documents.

Humans↗

PubMedDate not supplied

[Magnetic brain stimulation in treatment of depression: the search for effective parameters of stimulation].

Transcranial magnetic stimulation seems to evoke an antidepressant effect similar to that of electroshocks. This effect, however, is not accompanied by convulsions. This technique may be regarded as an alternative method of treatment of depression. It may prove as effective as electroshock treatment, but induce no negative phenomena connected with electro-convulsive treatment. Effectiveness of magnetic stimulation depends on selection of physical parameters of the applied magnetic field. In our model the optimum parameters of transcranial stimulation are: magnetic field induction B < or = 2 T stimulation frequency f [symbol: see text]¿50-100 Hz¿ time of single impulse twide [symbol: see text]¿0,1-1 ms¿ time of impulse rise trise [symbol: see text]¿(10)50-200 microseconds¿ time of impulse decline tdecline [symbol: see text]¿(100)200-1000 microseconds¿ total stimulation time ttotal > or = 1 min repetition rate repetition rate = 8-12

Animals↗