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Biomedical subjects

A Krause

Publications and source records attributed to A Krause.

At least 199 records · Page 11Linked to original sources
PubMed1987

Pseudocholinesterase variation in southern African populations.

Genetic variation at both the E1 and E2 loci of pseudocholinesterase was studied in 7 southern African populations. In the Ashkenazim and Afrikaners, E1 locus variation was observed and the frequency of the E1a or 'atypical' allele was found to be 0.017 in the Ashkenazim and 0.016 in the Afrikaans-speaking population, similar to those observed in other caucasoid populations. The E1s, or 'silent' allele, was found to occur in the Afrikaans-speaking population at a frequency 2-3 times greater than in other Caucasians, giving an increased risk of scoline-sensitivity. The rarity of the E1a in the Negro and Khoisan populations was confirmed. All 7 populations showed variation at the E2 locus with high frequencies of the E2+ allele in the Caucasians and San, while Negroes showed relatively low frequencies. Coloureds showed intermediate frequencies, consistent with their historical origins.

Alleles↗
PubMed1987

Red cell metabolism and ferritin levels in iron deficiency anaemia.

Basic red cell ferritin (RCF) content reflects the rate of iron uptake by marrow erythroid cells in patients with anaemia due to chronic inflammation which are sometimes also associated with metabolic disorders of the erythrocytes. For 29 patients with active inflammatic states of chronic rheumatoid arthritis (RA) and microcytic (mean corpuscular volume up to 80fl) or normocytic (MCV 80-95fl) anaemia respectively, the mean RCF content, irrespective of plasma ferritin levels, was determined using a recently established ELISA test. Red cell intermediates (ATP, GSH, 2.3 DP.G) were measured using conventional methods. The results revealed decreased RCF levels (2.8 +/- 1.5 ag/RBC) in 12 patients with RA and normal values (8.8 +/- 4.7 ag/RBC) in 17 patients which obviously did not correlate with the degree of the anaemia. The extent and pattern of the intermediates of RBC did not significantly vary from normal values. Thus, ATP, GSH and 2.3 DPT levels of RBC were only slightly increased up to 10%, especially in those patients with higher anaemic degrees. The findings of our study suggest that conventional indices for iron metabolic disorder in anaemic patients with chronic inflammatic disease should include peripheral microcytosis, transferrin saturation, and RCF content but could neglect plasma ferritin concentrations. Concerning the RBC metabolism this study did not disclose any further influences on iron metabolism parameters due to changes of mean cell age in patients with RA. Specific alterations which might hence produce additional functional disturbances of the erythrocytes in the peripheral microcirculation thus leading further to tissue cell damages in RA could be excluded as well.

Adult↗
PubMed1987

[Psychometric detection of depressive mood conditions].

An investigation into the diagnostic value of psychometric methods for the diagnosis of depressive states involved five psychometric questionnaires, one concentration test procedure, and one problem-solving approach to register cognitive processes. The data obtained for 80 depressive patients of varied aetiology were compared with those of sample of 80 neurotics without depressive aberration. Even univariate comparisons revealed statistically significant differences between the two samples. Multivariate methods (factor analysis, discriminative analysis) yielded more differentiated information. The "Scale for depression depth measurements" was found to be of particular diagnostic relevance; it was standardized for the time being.

Adult↗
PubMed1987

Class II MHC antigen (HLA-DR3) predisposes to sarcoid arthritis.

Recent studies indicate that the susceptibility to various inflammatory rheumatic diseases is an inherited trait determined by gene products of the class II histocompatibility complex (HLA-DR determinants). In a study designed to evaluate the concept of inherited susceptibility to sarcoid arthritis (SA), 42 patients with histologically proved acute disease underwent typing of HLA-A, -B, -C and -DR antigens. Using the microdroplet assay of human serum cytotoxins, we employed 156 antiserums to identify 52 antigens on A, B and C loci and 35 to identify 7 DR antigens on the surface of B cells. An ethnically matched control group consisted of 134 healthy volunteers. The frequency of B cell isoantigen DR3 specificity was significantly increased in patients with SA (relative risk, 4.8); HLA-DR3 was found in 25 (60%) of the patients, compared with 31 (23%) of the controls. This study lends further support to the hypothesis that the putative role of an infectious agent triggering SA cannot be judged without considering genetic cofactors.

Adult↗
PubMed1986

The upstream operator of the Escherichia coli galactose operon is sufficient for repression of transcription initiated at the cyclic AMP-stimulated promoter.

Two operators are known to bind Escherichia coli galactose repressor with roughly equal affinity. A study of the control these two operators exert on the two overlapping gal promoters is reported. The experiments rest on a set of mutations specifically constructed to inactivate individual control units of the gal operon and on quantitation of gal promoter activities. Messenger RNAs initiated at one or other of the promoters in a cell-free transcription-translation system were determined by a primer extension assay with synthetic deoxyoligonucleotide primers. The main conclusions are: (i) the classical galactose operator O1, located upstream with respect to the two overlapping promoters is sufficient for negative control of the cAMP activated promoter P1; (ii) complete repression of the second promoter P2, on the other hand, needs the presence of both intact operators O1 and O2. Thus, the two overlapping gal promoters (with only 5 bp separating their respective transcriptional start sites) are both subject to negative control by the galactose repressor. This regulation, however, is exerted by two different mechanisms.

Base Sequence↗
PubMed1985

[Evaluation of psychological stress before general anesthesia].

Pre-operative fear is not only a psychologically stressing phenomenon for the patient, but also a considerable risk factor due to its somatical correlative. In a study, 150 patients who were to undergo planned surgery under general anaesthesia were questioned about their fears and worries concerning the anaesthetic. Self-rating scales and rating scales by doctors and a complaints analysis curve were used in addition to an interview system on illnesses and specific previous experiences. 54% of the patients were, according to specific criteria, assessed as being anxious or very anxious (for example diffused anxiety, premature weakening of the anaesthetic effect, worry about not waking up out of the anaesthetic). Several factors which could have an anxiety-lessening or increasing effect and which are of importance for the psychological preparation for an operation were deduced from the investigation.

Adaptation, Psychological↗
PubMed1981

[Configuration-frequency-analytical assessment of psychometric procedures in brain damage diagnosis].

In a study of 462 neurological patients, the psychodiagnostic procedures of a tachistoscopic examination of the figure-ground differentiation, test dz and Bourdon-Wiersma test were assessed configuration-frequency-analytically. It was found that the procedure configuration of conspicuous tachistoscopie findings with a distinct slowing-down in the Bourdon-Wiersma test as well as reduced values in the test d2 (GZ--F) among all examined configuration indicate most clearly a cerebro-organic impairment.

Brain Damage, Chronic↗
PubMed1979

Q fever endocarditis in the United States.

A patient with Q fever endocarditis, which is almost unknown in the United States, was followed for a total of 32 months; the study was begun 3 1/2 months before aortic valve replacement. Diagnosis was confirmed by serology, visualization of Coxiella burnetii in excised aortic valve tissue by direct and immunofluorescence staining, and isolation of C. burnetii from aortic valve tissue. Serum antibodies against phase I and phase II antigens of C. burnetii were identified. Almost all phase I and phase II antibodies were IgG. These findings are compared with those in an uncomplicated case of acute Q fever. New findings on the immune response to chronic Q fever are presented.

Animals↗