Hospital hygiene in the German Democratic Republic.
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Cross Infection↗
Biomedical subjects
A Kramer
Publications and source records attributed to A Kramer.
Simultaneous synthesis of peptide libraries on single resin and continuous cellulose membrane supports: examples for the identification of protein, metal and DNA binding peptide mixtures.
Peptide libraries were simultaneously synthesized on single supports by double coupling 0.8 equivalents of an equimolar acylating amino acid mixture consisting of 19 amino acids (cysteine omitted) at randomized sites, thus compensating for the different coupling rates of the amino acids. Peptide epitope mixtures, as well as very complex mixtures such as a completely randomized hexapeptide, were prepared and analyzed by HPLC and amino acid analysis. The results obtained indicate that this method can be applied to the synthesis of peptide libraries. Parts of a simultaneously synthesized solution phase combinatorial library XXB1B2XX were successfully used for the detection of the linear epitope HFND of transforming growth factor-alpha (TGF alpha) recognized by the monoclonal antibody Tab2. Furthermore, novel combinatorial peptide libraries XXB1B2XX were prepared on continuous cellulose membrane supports, also allowing the identification of TGF alpha epitope sequences. In addition, peptide mixtures that bound to a double-stranded DNA (15mer) and silver were identified. These preliminary results indicate that cellulose-bound combinatorial peptide libraries can be used for the rapid and inexpensive screening of millions of peptides to identify single molecules that bind any given ligand such as proteins, nucleic acids and metals.
Amino Acid Sequence↗
[Promotion of wool growth and body weight development of sheep by alimentary supplementation with thiocyanate].
Adult sheep were administered 12 mg thiocyanate (SCN-)/kg body weight orally twice a year in altogether 16 respectively 41 single doses under conventional keeping during the period of stabling (winter) in two consecutive years. Fattening lambs got an analogous SCN- dosage with altogether 4 respectively 34 single doses under intensive keeping. Aided by this temporarily obtained balance of an alimentary SCN- deficiency the following effects were reached during conventional keeping: Significant increase of the wool mass in the greasy state and a tendency to better breading performance. During intensive fattening an increase of the wool mass and of the staple cross-section was proven statistically in the mean for all the test animals, but for the increase of the staple length it could be shown only by comparison of twins.
Aging↗