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Biomedical subjects

A Klopper

Publications and source records attributed to A Klopper.

At least 109 records · Page 6Linked to original sources
PubMedDate not supplied

Observations on the separation of SP1 alpha and SP1 beta by affinity chromatography.

SP1 antigens in late pregnancy blood, in placental extracts and in acidified plasma have been purified on positive affinity chromatography columns with immobilized SP1 antiserum and on negative affinity columns with immobilized antihuman non-pregnancy serum. Both SP1 alpha and SP1 beta are bound by the positive column but only SP1 alpha is bound by the negative column. This is adduced as further support for the view that SP1 alpha is formed by the combination of SP1 beta with a protein present in non-pregnancy serum.

Chromatography, Affinity↗
PubMedDate not supplied

The demonstration of two pregnancy-associated proteins with SP1 determinants in placental extracts.

The SP1 content of the placenta has been examined. It was found that, as in pregnancy serum, two variants of the protein existed in the placenta. The smaller molecule, with a beta 1 electrophoretic mobility, is readily extractable with phosphate buffered saline. The second larger molecule is more tightly bound in the placenta and requires Triton X-100 to be solubilized. The hypothesis is put forward that the larger alpha variant is the precursor form which is related more irregularly and in a smaller amount from the placenta into the maternal circulation.

Blood Proteins↗