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Biomedical subjects

A Klein

Publications and source records attributed to A Klein.

At least 397 records · Page 22Linked to original sources
PubMed1981

Dialysis for psoriasis - preliminary remarks concerning mode of action.

In one patient treated by peritoneal dialysis for psoriasis the skin lesions cleared completely. In two persons hemodialysis gave unsatisfactory results. In treated and another three untreated patients cellular immunity was evidently suppressed. This phenomenon is similar to the immunologic changes in persons with uremia. Solutes in middle molecular weight range (SMMWR) suppress cellular immunity. These compounds were therefore investigated in psoriatics. The level of SMMWR was lower before than after each dialysis, although their concentration increased slightly during the dialytic treatment. This suggests that solutes in middle molecular weight are fixed in the epidermis and/or in the walls of the skin capillaries and that dialysis liberates them from these places. Peritoneal dialysis seems to be a more effective method of treating psoriasis than hemodialysis. Solutes in middle molecular weight range might play an important role in the pathomechanisms of this multifactorial disease.

Humans↗
PubMed1981

Histiocytosis X of the head and neck.

This paper reviews retrospectively the otolaryngological manifestations of 48 cases of histiocytosis X treated at The Montreal Children's Hospital. Age incidence in these cases compared with other series; and ther was a female preponderance. If temporal bone lesions plus other skull lesions are included in otolaryngological manifestations of histiocytosis X, then on workup evidence of disease was found in the head and neck in 38 of 48 patients (80 per cent). An ENT problem was the presenting compliant in only some of these. If only temporal bone lesions are included, then 17 of 48 (40 per cent) had head and neck involvement. The prognosis became poorer as the disease progressed.

Age Factors↗
PubMed1980

Studies on the functions of DNA helicase I and DNA helicase II of Escherichia coli.

Inactivating antibodies raised against DNA helicase I and DNA helicase II were applied to Escherichia coli DNA-replicating systems. Antibody against DNA helicase II was found to inhibit the replication of E. coli DNA, lambda phage DNA (during early and late phases), and ColE1 plasmid DNA during the elongation step. The antibody did not inhibit the replication of fd replicative form (RF) DNA, the unwinding of which is known to depend on the rep protein. Antibody against DNA helicase I failed to inhibit any of the replication processes. The replication of E. coli DNA, lambda-DNA, and ColE1 DNA is known to be initiated in a closed circle, in contrast to fd RF which is known to be initiated in a nicked circle. In conjunction with data given in the literature, our results suggest that replicative unwinding is carried out by DNA helicase II or rep protein, depending on the mechanism by which DNA replication is initiated. The concentration of DNA helicase II in E. coli, as determined by immunological methods, is 5000 to 8000 copies/cell; that of DNA helicase I is 500 to 700.

Adenosine Triphosphatases↗
PubMed1980

Intrapericardial teratoma in infancy.

Intrapericardial teratoma in the newborn is a rare potentially fatal neoplasm. Pre-operative diagnosis depends upon recognition of specific radiographic and echocardiographic findings in a newborn with a larg cardiothymic image and pericardial effusion. Surgical intervention is usually curative whereas undiagnosed intrapericardial teratoma is often fatal.

Angiocardiography↗
PubMed1980

Cloning of the replication gene O of E. coli bacteriophage lambda and its expression under the control of the lac promoter.

The expression of the replication gene O of bacteriophage lambda was put under the control of the lac promoter-operator region integrated into the pBR322 cloning vehicle. The new plasmid pKK104 was introduced into minicells and the O gene induced by isopropyl-beta-thiogalactoside (IPTG). The O protein could be identified as a major component in extracts from these cells, in association with the cell membrane fractions. The molecular weight of the O protein in SDS gels is about 33 000, and it is metabolically unstable but apparently stable upon isolation as a membrane-associated fraction.

Bacteriophage lambda↗
PubMed1980

Influence of middle-molecular-weight solutes from dialysate on the migration rate of leukocytes.

To investigate further the biologic activity of solutes in the middle-molecular-weight range, we studied the influence of these compounds on the migration of unseparated white blood cells and separated granulocytes and lymphocytes. Middle molecules (MM) inhibit the migration of unseparated leukocytes, but this effect on lymphocytes was seldom observed. With scanning electron microscopy, unseparated leukocytes were shown to adhere to one another, forming cellular clumps. This phenomenon could not be seen when separated cells were used. These results give some insight into the mechanism of inhibition exerted by MM and into the changes, observed in earlier experiments, of cellular composition of skin exudate obtained from untreated patients with uremia.

Cell Aggregation↗
PubMed1980

Effect of tumor removal on the presence of lymphocytic cortisol metabolism-enhancing factor in plasma of cancer patients.

Because lymphocytic cortisol metabolism-enhancing factor (LCMEF) is absent in the plasma of cancer patients (CP), this study was undertaken to determine the influence of tumor removal on this phenomenon. Known concentrations of human lymphocytes were incubated with cortisol in media containing 50% phosphate-buffered saline (PBS) and 50% of one of the following: a) homologic normal plasma (HP),b) plasma from patients with noncancerous diseases (NCD) before surgery, c) plasma from patients with NCD after surgery, d) plasma from CP before tumor removal, e) plasma from CP after tumor removal, f) plasma from long-surviving CP (LSCP), and g) PBS. With the exception of plasma from the LSCP group, all the plasma had the capacity to enhance the lymphocytic cortisol metabolism (LCM) when compared with that of PBS. There was no significant difference between the metabolism obtained with HP and that obtained with plasma from patients with NCD either before or after surgery. The plasma from CP led to a significant reduction in activity, with no significant difference in conversion rates before and after tumor removal. The plasma from LSCP failed to enhance LCM, had a conversion rate similar to that of PBS and significantly lower than that of the plasma from CP, and appeared to contain no LCMEF. These findings, which showed that the lack of LCMEF in CP is not influenced by tumor removal, may indicate 1) that the lack of LCMEF preceded the appearance of cancer or 2) irreversibility of a possible anti-LCMEF synthesis effect was induced by the tumor.

Biological Products↗
PubMed1980

Isolation of a complex between the P protein of phage lambda and the dnaB protein of Escherichia coli.

P protein of phage lambda and dnaB protein of Escherichia coli were isolated from (a) bacteria containing an inducible lambda P gene on a plasmid, and (b) phage-lambda-infected bacteria. P protein from both sources copurifies with part of the dnaB protein during four purification steps. A highly purified preparation contains the multimeric dnaB and the P protein in a complex as revealed by glycerol gradient centrifugation. The complex is composed of two major polypeptides. Their molecular weights of 52 000 and 26 000 are identical to those previously determined for the dnaB and P polypeptides, respectively. The complex contains a DNA-dependent ribonucleoside triphosphatase activity which can be inactivated by anti-dnaB globulin. Both the dnaB complementing and the ribonucleoside triphosphatase activities are partially masked by the P protein as shown by their stimulation following a treatment with sodium chloride and N-ethylmaleimide.

Ammonium Sulfate↗