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Biomedical subjects

A Klein

Publications and source records attributed to A Klein.

At least 361 records · Page 20Linked to original sources
PubMed1986

Dorsal dislocation of the radiocarpal joint with associated dorsal perilunar dislocation.

Radiocarpal dislocations are uncommon injuries that occur even more rarely in association with intercarpal dislocations. All the reported examples of such intercarpal dislocations are volar. We here describe a patient with a unique dorsal radiocarpal dislocation with an additional dorsal perilunar dislocation. Radiographic identification of this injury has important implications for its successful treatment.

Adult↗
PubMed1986

Sera of patients with cancer or cirrhosis and of newborns exhibiting inhibitory effects on the metabolism of cortisol by lymphocytes.

The effect was examined of ethanol extracts of normal sera (NS), cancer patients' sera (CPS), cirrhosis patients' sera (CirPS), and umbilical cord sera (UCS) on cortisol metabolism by lymphocytes (CML). All the ethanol extracts of these sera showed an inhibitory effect on CML. The most significant inhibition was obtained with CPS and CirPS followed by UCS. A significant inhibition of NS was obtained after doubling their equivalent volume.

Ethanol↗
PubMed1985

Termination of a transcription unit comprising highly expressed genes in the archaebacterium Methanococcus voltae.

The 3'termini of transcripts originating from genes organized in a highly expressed transcription unit were analyzed in the archaebacterium Methanococcus voltae. The putative termination signals were found in an AT-rich intergenic region following the 3'-terminal gene. The two detected signals both contain oligo(T) sequences. A possible stem/loop structure immediately precedes one of the oligo(T) tracts. This secondary structure is considered to have an additional function in stabilizing the transcripts.

Bacteriophage lambda↗
PubMed1985

Non-repetitive AT-rich sequences are found in intergenic regions of Methanococcus voltae DNA.

AT-rich DNA, which constitutes a distinct fraction of the cellular DNA of the archaebacterium Methanococcus voltae, was shown to consist of non-repetitive sequences dispersed on the chromosome and to lack continuous open reading frames in five out of six randomly analyzed cases. Upon subsequent analysis of intergenic regions, AT-rich sequences were again detected. Transcription start points were mapped in front of three open reading frames. The 5' ends of the transcripts were found in variable positions relative to the AT-rich sequences in the different cases. Shine-Dalgarno type sequences complementary to the 3' end of 16S rRNA were discovered at suitable distances from the 5' ends of the genes.

Base Sequence↗
PubMed1985

"V" shaped predens space.

"V" shaped widening of the predens space (PDS) in flexion can be a worrisome finding in trauma patients, possibly representing injury to the transverse ligament. These patients may also show widening of the C-1/C-2 interspinous distance. We think this appearance is usually due to increased flexion mobility at the atlantoaxial level with developmental elongation or laxity of the cranial end of the transverse ligament and/or the posterior ligamentous complex. Tearing of only the cranial end of the transverse ligament must be extremely rare, if it occurs at all; there is no reported proven case. Tearing of only posterior ligaments seems possible and should be evaluated clinically.

Adolescent↗
PubMed1985

Mexiletine kinetics in healthy subjects taking cimetidine.

Cimetidine, a commonly used H2-receptor antagonist, was found to interact adversely with many drugs, including class I antiarrhythmics such as lidocaine and quinidine. To test the effect of cimetidine on the kinetics of mexiletine, a class I antiarrhythmic similar to lidocaine, the absorption and disposition of mexiletine were followed in six healthy subjects before and after 1 week of cimetidine, 300 mg by mouth four times a day. Cimetidine did not alter the distribution and elimination of mexiletine, as shown by similar mean kinetics including total body clearance, AUC, and the elimination t1/2 before and after cimetidine treatment. Cimetidine did have a significant effect on mexiletine absorption, as demonstrated by a longer mean absorption t1/2 (from 0.20 +/- 0.14 to 0.61 +/- 0.35 hours), a longer mean time to peak mexiletine concentration (from 1.13 +/- 0.31 to 1.88 +/- 0.83 hours), and decreased mexiletine plasma concentration (from 0.74 +/- 0.19 to 0.59 +/- 0.15 mg/ml). We conclude that cimetidine does not alter the disposition of oral mexiletine in normal subjects.

Absorption↗
PubMed1985

Stability of tryptophan during food processing and storage. 2. A comparison of methods used for the measurement of tryptophan losses in processed foods.

Tryptophan losses in stored milk powders and in different model systems representing the major reactions of food proteins during processing and storage were determined using four different chemical methods and in a rat assay. Similar tryptophan values were obtained by the three chemical methods which included high pressure liquid chromatography (HPLC) after sodium hydroxide hydrolysis, colorimetric reaction with p-dimethylamino-benzaldehyde (p-DAB) after barium hydroxide hydrolysis, and fluorescence of the Norharman derivative after NaOH hydrolysis. Tryptophan losses in the treated proteins as measured by the alkaline-hydrolysis methods were generally smaller than those determined by the rat assay. Good agreement however was obtained when the chemical value was multiplied by the true nitrogen digestibility. Determination of tryptophan by reaction with p-DAB after papain (EC 3.4.22.2) digestion gave lower values in the processed proteins than the other chemical methods or the rat assay. A method using alkaline-hydrolysis is recommended, preferably combined with HPLC-measurement of the liberated tryptophan.

Animals↗
PubMed1985

Transforming growth factors (TGFs) produced by R-XC cells.

Chemical fractionation as a first step of purification of TGFs from R-XC cells and culture medium was described. Physicochemical treatment indicated that the crude preparation of RGF's contains a heat and acid stable and dithiothreitol and trypsin sensitive polypeptides related with the alpha and/or beta TGFs. The influence of partially purified TGFs on REF cell colony formation in soft agar was documented.

Animals↗
PubMed1985

Isolation of the homogeneous constituents from non-diffusible sugar-peptide (NSP) fraction originating from bovine plasma. IV. The influence on cell growth of REF and R-XC cells in vitro.

The effect of the NSP fraction and its four peptide constituents (A, C2, D and G) on the 3H-thymidine incorporation into REF and R-XC cells in vitro was described. Only peptide G (at concentration 5 micrograms/ml) affected the growth of the investigated cells, decreasing the 3H-thymidine uptake to 4-13% relative to untreated control, regardless of the kind of investigated cells. The effect of peptide G, added in concentration 10 ng-1 microgram, however was dependent on the type of investigated cells, suggests that neoplastic transformation changes the response of rat cells in vitro. The possible explanation of the differences in the biological response of the investigated cells to peptide G is proposed.

Animals↗
PubMed1984

Primary structure determination of five sialylated oligosaccharides derived from bronchial mucus glycoproteins of patients suffering from cystic fibrosis. The occurrence of the NeuAc alpha(2----3)Gal beta(1----4)[Fuc alpha(1----3)] GlcNAc beta(1----.) structural element revealed by 500-MHz 1H NMR spectroscopy.

The structure of sialylated carbohydrate units of bronchial mucins obtained from cystic fibrosis patients was investigated by 500-MHz 1H NMR spectroscopy in conjunction with sugar analysis. After subjecting the mucins to alkaline borohydride degradation, sialylated oligosaccharide-alditols were isolated by anion-exchange chromatography and fractionated by high performance liquid chromatography. Five compounds could be obtained in a rather pure state; their structures were established as the following: A-1, NeuAc alpha(2----3)Gal beta(1----4) [Fuc alpha(1----3)]GlcNAc beta(1----3)Gal-NAc-ol; A-2, NeuAc alpha(2----3)Gal beta(1----4)GlcNAc beta(1----6)-[GlcNAc beta (1----3)]GalNAc-o1; A-3, NeuAc alpha(2----3)Gal beta-(1----4)[Fuc alpha(1----3)]GlcNAc beta(1----3)Gal beta(1----3) GalNAc-o1; A-4, NeuAc alpha(2----3)Gal beta(1----4)[Fuc alpha(1----3)]Glc-NAc NAc beta(1----6)[GlcNAc beta(1----3)]GalNAc-o1; A-6,NeuAc alpha-(2----3) Gal beta(1----4)[Fuc alpha(1----3)]GlcNAc beta(1----6)[Gal beta-(1----4) GlcNAc beta(1----3)]GalNAc-o1. The simultaneous presence of sialic acid in alpha(2----3)-linkage to Gal and fucose in alpha(1----3)-linkage to GlcNAc of the same N-acetyllactosamine unit could be adequately proved by high resolution 1H NMR spectroscopy. This sequence constitutes a novel structural element for mucins.

Bronchi↗
PubMed1984

A multigene family of intron lacking and containing genes, encoding for mouse ribosomal protein L7.

Mouse ribosomal protein L7 is encoded by a multigene family. Screening of two mouse genomic libraries with cloned L7 cDNA, has resulted in the isolation of nine independent lambda Charon 4A recombinant phages which include seven different L7 genes. Restriction enzyme mapping of six of these genes (L7-1, L7-16, L7-18, L7-28, L7-35 and L7- 16b ) reveals dissimilarity in sites within the L7 sequences as well as in the flanking regions. Electron microscopic analysis of heteroduplex and S1 nuclease mapping demonstrate that the first five genes contain the entire L7 mRNA sequence but lack introns. Based on these features we propose that these are processed genes. Of the L7 genes described here only one (L7- 16b ) exhibits a high degree of homology with L7 mRNA and contains introns. We discuss the possibility that this low representation of intron containing L7 genes may reflect the proportion of functional L7 genes in this multigene family.

Animals↗