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Biomedical subjects

A Kikuchi

Publications and source records attributed to A Kikuchi.

At least 433 records · Page 24Linked to original sources

[In-vitro motility studies on the strips of the canine gallbladder (author's transl)].

In-vitro motility of the gallbladder was investigated in the circularly cut full thickness strips obtained from 19 mongrel dogs. The present study was undertaken to determine an appropriate size of the strips for observation and to know any difference, if exists, between motilities of the strips prepared from the neck, body and fundus. Recordings were made on a pen chart recorder using an isometric tension system. The results obtained were as follows. 1) The most appropriate size of the specimens to examine the changes of contractile dynamics was 5 mm in width and 15 mm in length. 2) The strips from three parts of the gallbladder showed similar rhythmic and tonic spontaneous contraction patterns.

Animals↗

Gene expression of an Escherichia coli ribosomal RNA promoter fused to structural genes of the galactose operon.

The promoter region of the rrnB ribosomal RNA gene of Escherichia coli has been ligated within the epimerase gene (galE) of the galactose operon in a lambda phage vector. The recombinant lambda phage has been characterized by restriction mapping and assays of both galK (galactokinase) gene activity and galactose messenger RNA hybridization. In such lyosgens, expression of the fused galactose operon occurs as a function of growth rate in a manner characteristic of ribosomal RNA gene expression and is subject to stringent control by amino acid availability for protein synthesis. Galactose messenger RNA arising from the ribosomal promoter is not as metabolically stable as ribosomal RNA.

Bacteriophage lambda↗