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Biomedical subjects

A Kiessling

Publications and source records attributed to A Kiessling.

At least 19 recordsLinked to original sources
PubMed2004

Identification of an HLA-A*0201-restricted T-cell epitope derived from the prostate cancer-associated protein prostein.

The development of T-cell-based immunotherapies of cancer largely depends on the availability of tumour-associated antigens capable of eliciting tumour-directed cytotoxic T-cell responses. In prostate cancer, the number of antigens defined as suitable targets of cytotoxic T lymphocytes (CTLs) is still limited. Recently, prostein was identified as a transmembrane protein that is highly restricted to prostate tissues. In our study, prostein transcripts were found to be abundant in both malignant and nonmalignant prostate tissue samples. To identify immunogenic CD8+ T-cell epitopes, human leucocyte antigen-A(*)0201-binding peptides were selected from the amino-acid sequence of prostein and were used for the in vitro stimulation of CD8+ T lymphocytes. Specific CTLs were raised against the prostein-derived peptide CLAAGITYV that were capable of lysing prostate cancer cells, indicating that this peptide is naturally generated by tumour cells. Our data suggest that prostein is a suitable candidate to be included in a T-cell-based immunotherapy of prostate cancer.

Aged↗
PubMed2003

The pattern of urinary acylcarnitines determined by electrospray mass spectrometry: a new tool in the diagnosis of diabetes mellitus.

l-Carnitine and its esters are products of intermediary metabolism of organisms. The distribution pattern or the favored excretion of individual acylcarnitines tells something about metabolic diseases. The determination of the urinary acylcarnitine pattern by flow injection analysis (FIA)-electrospray ionization (ESI)-mass spectrometry (MS) is presented. Groups of healthy probands and patients suffering from diabetes mellitus were investigated due to their significant acylcarnitine profile. The statistical analysis of data sets obtained clearly shows a difference in the acylcarnitine pattern of healthy and sick probands. In comparison to the controls, diabetes mellitus patients excrete more long-chain carnitine esters ranging from dodecanoyl to palmitoylcarnitine. Thus, the urinary acylcarnitine pattern determined by ESI-MS can be a useful tool in the diagnosis and therapy monitoring of diabetes mellitus.

Adult↗
PubMed2001

Note on a method for individual recognition in feed pecking in free running groups of hens.

A system for automatic registration and individual recognition of feed pecking (activity and quantity) in groups of free running hens was tested. A PIT (Passive Integrated Transponder)-tag system was used to separate and register individuals when they were feeding. An electronic balance system placed under the feeder registered the amount eaten by each individual on a PC. A test with two different feed stuffs; oat and wheat was performed on three hens during a total of 6 weeks.

Journal Article↗
PubMed2001

Effects of high intensity exercise training on cardiovascular function, oxygen uptake, internal oxygen transport and osmotic balance in chinook salmon (Oncorhynchus tshawytscha) during critical speed swimming.

To examine cardiorespiratory plasticity, cardiovascular function, oxygen consumption, oxygen delivery and osmotic balance were measured at velocities up to critical swimming speed (Ucrit) in seawater-adapted chinook salmon. We used two groups of fish. The control group had swum continuously for 4 months at a low intensity (0.5 BLs(-1)) and the other was given a high-intensity training regimen (a Ucrit swim test on alternate days) over the same period of time. Compared with available data for other salmonid species, the control group had a higher maximum oxygen consumption (MO2max; 244 micromol O2 min(-1) kg(-1)), cardiac output (Qmax; 65 ml min(-1) kg(-1)) and blood oxygen content (CaO2; 15 ml O2 dl(-1)). Exercise training caused a 50% increase in MO2max without changing either Ucrit or CaO2, even though there were small but significant increases in hematocrit, hemoglobin concentration and relative ventricular mass. During swimming, however, exercise-trained fish experienced a smaller decrease in body mass and muscle moisture, a smaller increase in plasma osmolality, and reduced venous oxygen stores compared with control fish. Consequently, exercise training apparently diminished the osmo-respiratory compromise, but improved oxygen extraction at the tissues. We conclude that the training-induced increase in MO2max provided benefits to systems other than the locomotory system, such as osmoregulation, enabling trained fish to better multitask physiological functions while swimming. Furthermore, because a good interspecific correlation exists between MO2max and arterial oxygen supply (TO2max; r2=0.99) among temperate fish species, it is likely that CaO2 and Qmax are principal loci for cardiorespiratory evolutionary adaptation but not for intraspecific cardiorepiratory plasticity as revealed by high intensity exercise training.

Animals↗
PubMed1995

Parameters that predict success for natural cycle in vitro fertilization-embryo transfer.

OBJECTIVES: To analyze our natural cycle IVF-ET experience in order to identify parameters predictive for success. DESIGN: Retrospective study. SETTING: Private-based reproductive center. PATIENTS: All patients who presented for IVF-ET interested in unmedicated cycles. MAIN OUTCOME MEASURES: Routine monitoring for IVF-ET was performed. In addition, serum values for FSH, PRL, E2, and P were analyzed. RESULTS: Day 3 FSH values were not useful, whereas elevated day 3 LH values adversely correlated with outcome. Age was the single most important predictor of success. With two cycles, 87.5% of pregnancies occurred. CONCLUSION: With proper patient selection, natural cycle IVF-ET results can be substantially improved.

Adult↗
PubMed1989

In vitro selective glycogen depletion of frog twitch and slow muscle fibres.

Glycogen as measured histochemically with periodic acid-Schiff (PAS) reaction was depleted in frog muscle fibres following electrical stimulation of the nerve. The types of the muscle fibres examined were identified in serial cryostat sections. We used appropriate stimulation regimes which provide a heavy work load only for twitch fibres (at low stimulation frequency) or selectively for slow fibres (contractures in response to high frequency stimulation or pharmacological agents). Twitch and slow fibres lost their glycogen depending on the work load.

Animals↗
PubMed1988

FSH-induced ovulation in intact and hypophysectomized mice.

A single, ovulatory dose of 25 micrograms of a highly purified preparation of ovine FSH caused ovulation in 89% of hypophysectomized and 91% of intact female mice primed 48 h earlier with PMSG; the number of oocytes recovered (29.4 +/- 4.7 and 22 +/- 2.7/mouse ovulating, respectively) compared favourably with the 20.0 +/- 2.9 oocytes per ovulating female recovered from animals that received PMSG + hCG. After oFSH injection, 82% of oocytes released were fertilized and developed to blastocysts. That the trace contamination (less than 0.2%) of the oFSH with oLH was not responsible for the ovulation was shown by the markedly reduced number of oocytes collected from ovulating females that were injected with equivalent low levels of hCG (0.001 micrograms) or oLH (1 microgram) (9.0 +/- 3.3 and 8.0 +/- 3.1, respectively). These results demonstrate that oFSH is as effective as LH in inducing ovulation of competent oocytes in the mouse.

Animals↗
PubMed1984

Fibre composition and enzyme activities in five different muscles from the Svalbard reindeer.

beta R fibres (type I) constitute less than 10% of the semimembranosus and longissimus dorsi muscles and about twice as much of the gluteobiceps and flexor hallucis. Except for longissimus dorsi, 50% or more consist of alpha W (type IIB) fibres--in semimembranous, as much as 70%. Despite the comparatively large content of alpha W fibres, both the oxidative capacity and the capacity to metabolize fatty acids is high. Furthermore, unexpectedly small differences in oxidative capacity between the three fibre types beta R, alpha R and alpha W (I, IIA and IIB) are revealed by histochemical staining. These results indicate a tendency to bring the three fibre types closer together as regards metabolic activities, as an adaptation to the relatively tranquil life of this animal. However, the large content of alpha W fibres does not accord well with this way of life, as they guarantee quick movements. The comparatively high oxidative capacity of the alpha W fibres in the Svalbard reindeer and the fact that during starvation it is primarily alpha W fibres that contribute to the energy supply by protein degradation may nevertheless account for their abundant occurrence.

3-Hydroxyacyl CoA Dehydrogenases↗
PubMed1976

[Diameter changes of muscle fiber types of the inferior oblique muscle of the rabbit following denervation].

Changes in fibre diameters of extraocular muscles of the rabbit were studied at different times after denervation. The whole inferior oblique muscle hypertrophied, while some of the muscle fibres hypertrophied and others showed atrophy, depending on the fibre type. Fibre types have been determined by their histochemical enzyme profile. In the central layer of the muscle the phasic muscle fibres, which are rich in mitochondria, exhibited a transient hypertrophy being maximal 4-5 weeks after denervation and afterwards they atrophied; other phasic muscle fibres, which are poor in mitochondria, atrophied without having shown any sign of hypertrophy. Special, putatively slow tonic muscle fibres, which have low enzyme activities, underwent small long-lasting increases of their diameters. In the superficial layer of extraocular muscle there are two types of extremely thin muscle fibres rich in mitochondira. Both these fibre types hypertrophied to the greatest degree and for a very long time. Comparable changes in fibre diameters as described here for the muscle fibre types of an extraocular muscle are known from special muscle fibres in other vertebrate

Adenosine Triphosphatases↗
PubMed1975

[Evidence and meaning of monophasic potentials in the electromyogram of extraocular muscles of rabbits and cats (author's transl)].

The EMG of extraocular muscles of rabbits and cats was recorded by a concentric-needle electrode provided for clinical use. The EMG showed spike activity (at least biphasic, fast waves of 1 to 2 msec duration) and characteristic long-duration monophasic waves (decline for up to 7 msec). The monophasic potentials are interpreted as local depolarizations arising at the nerve-muscle junctions of multiply innervated muscle fibers. Spike- and monophasic potentials are discharged in independent rhythms whose frequency changes when the head position is altered. The frequency of spike- and monophasic potentials are changed in the same direction by labyrinthine reflexes. Spontaneous monophasic potentials recruit at a lower threshold.

Action Potentials↗
PubMed1975

[Non-propagated potentials in the electromyogram of mammalian ocular muscles].

Electromyograms of mammalian extraocular muscles were recorded by means of a coaxial electrode. Besides normal extracellular spike potentials (1-2 msec duration), monophasic waves (with a decline lasting up to 7 msec) were recorded. As to the interpretation of these potential changes in terms of a potential drop that is produced by local currents flowing from the resting region of a fibre towards the active region consideration is given to two cases. First, a propagated active region (spike potentials, at least diphasic) and second, a stationary active region (with resulting monophasic waves). In the EMGs spontaneous monophasic potentials recruit at a lower threshold than spike potentials; frequency changes were observed when head position was altered. The latter are interpreted as local depolarizations occurring at neuromuscular junctions of multiple innervated muscle fibres among those fibre types that compose extraocular muscles.

Action Potentials↗