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A Kessel

Publications and source records attributed to A Kessel.

46 records · Page 3Linked to original sources
PubMed1994

Increased expression of pp60c-src in gastric mucosa of aged rats.

The relationship between proliferative activity and the expression of pp60c-src in gastric mucosa (oxyntic gland area) of young (4-month) and aged (24-month) Fischer 344 rats was examined. Gastric mucosal proliferative activity, as assessed by 5-bromo-2'-deoxyuridine (BrdU) immunoreactive cells, was found to be 115% (p < .001) higher in aged than in young rats. This was associated with a 47% rise (p < .025) in overall tyrosine kinase (Tyr-k) activity and a 5-7-fold increase in autophosphorylation of four prominent protein bands with M(r) of 40, 55, 60, and 70 kDa in gastric mucosal membranes. A similar phenomenon was also observed for Tyr-k activity of pp60c-src in that the aged rats revealed a 69% (p < .025) higher enzyme activity and a 5-fold rise in the extent of autophosphorylation of this protein when compared with the corresponding values from young animals. Increased Tyr-k activity of pp60c-src in the gastric mucosa of aged rats could in part be due to higher levels of this protein because the relative concentration of pp60c-src, as assessed by Western blot analysis, showed a 2-5-fold increase over the young animals. In addition, the relative concentration of c-src mRNA in the gastric mucosa of aged rats was also found to be 5-6-fold higher than in young animals. We suggest that pp60c-src may play a role in regulating gastric mucosal proliferative processes in the gastric mucosa of aged rats.

Aging↗
PubMedDate not supplied

The association of serum matrix metalloproteinases and their tissue inhibitor levels with scleroderma disease severity.

OBJECTIVE: Matrix metalloproteinase 3 (MMP-3) is reported to play an important role in the pathogenesis of systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). Studies have also investigated the association of different tissue inhibitors of MMPs (TIMPs) with fibrosis in scleroderma (SSc). The aim of this study was to evaluate the correlation of serum MMP-1, 3 and TIMP-1 with severity and disease specific markers of SSc and RA. METHODS: Serum MMP-1,3 and TIMP-1 were measured in 42 SSc patients (age range 28-68 yr mean 47 yr) and compared to 29 RA and 30 healthy age- and sex-matched individuals. Elevated values of MMPs and TIMP-1 were defined as those greater than 2 SD above the normal mean. All SSc and RA patients were scored for disease severity. RESULTS: Serum MMP-1 was significantly elevated in 8/42 (19%) SSc patients (p = 0.01) but only in 2/29 (7%) RA patients (p = 0.2). Whereas MMP-3 levels were elevated in 10/29 (34%) RA patients (p = 0.002), it was elevated in only 5/42 (12%) SSc patients (p = 0.03). TIMP-1 was found elevated in 17/42 (40%) SSc patients (p = 0.001) and in only 4/29 RA patients (with a strong trend towards significance, p = 0.052). We found a significant association between the elevation of both MMPs and TIMP-1 levels, with the severity of SSc. Those who had an increase of more than one MMP and/or TIMP, demonstrated life-threatening major organ involvement such as end stage lung fibrosis, GI aperislasis, and severe cardiacfailure. Contrary to that in SSc, the severity of RA showed some trend of association with MMP-3 only. CONCLUSION: We confirm previous observations that MMPs and TIMPs may play an important role in various rheumatic diseases. Whereas serum increase of MMP-3 correlated with RA severity, SSc severity was more characterized by the increase of both MMP-1 and TIMP-1. This suggests that the MMPs and TIMPs involved in SSc are different than those playing a role in RA, which may indicate that in SSc they are produced in different locations than in RA.

Adult↗