[The anchoring rod and stress priming as new principles in cementless prosthetic retention].
Explore the source record for details and available documents.
Biomechanical Phenomena↗
Biomedical subjects
A Keller
Publications and source records attributed to A Keller.
[Importance of Glasser's disease in swine].
Explore the source record for details and available documents.
Animals↗
Onset and development of neuron-specific enolase immunoreactivity in the peripheral vestibular system of the mouse.
The development of neuron-specific enolase (NSE) immunoreactivity in mouse sensory and ganglion vestibular cells was studied from gestation day 14 to adulthood. NSE staining appeared sequentially in these structures with a pattern that closely paralled their maturation sequences. The onset of NSE reactivity, at gestation day 15, in ganglion cells was concomitant with the formation of contacts between the afferent fibers and sensory cells, and was observed in hair cells, at gestation day 17, during the period in which the first synaptic structures form. The development of NSE staining in the cristae revealed an apex-base and axial gradients of maturation.
Animals↗
The identification of thalamocortical axon terminals in barrels of mouse Sml cortex using immunohistochemistry of anterogradely transported lectin (Phaseolus vulgaris-leucoagglutinin).
The anterograde transport and immunohistochemical demonstration of the lectin, Phaseolus vulgaris-leucoagglutinin (PHA-L) has been used to label thalamocortical axon terminals in barrels of mouse SmI cortex. The reaction product is visible with both the light and electron microscopes so that the distribution of axons and the types of synapses they form can be determined.
Afferent Pathways↗
High-dose cytosine arabinoside and mAMSA induction and consolidation in patients with previously untreated de novo acute nonlymphocytic leukemia: Phase I Pilot Study for the Eastern Cooperative Oncology Group.
Explore the source record for details and available documents.
Aminoacridines↗
[The interposition nail in diaphyseal bone defects and arthrodesis nail in failed knee-joint replacement].
Our clinical experience with different constructions of interposition and arthrodesis nails, used in 3 and 12 patients respectively, is presented. It has shown that cemented intramedullary nailing for the stabilization of long bones and the knee joint using an approach from the site of the defect is a useful additional means in reconstructive bone and joint surgery.
Adult↗
Pelvic floor reconstruction using the rectus abdominis muscle flap.
In the 2 cases reported the rectus abdominis muscle was used as a sling to construct the pelvic floor. In patient 1, a large squamous cell carcinoma of the perineum was treated with an abdominal perineal resection. Reconstruction was accomplished internally using a rectus abdominis muscle sling and externally with a gluteus maximus musculocutaneous flap. In the second case, the rectus abdominis muscle was used as a supportive sling to reinforce a perineal hernial repair and prevent recurrence.
Abdominal Muscles↗
The medial gastrocnemius muscle flap: a local free flap.
A new application of the medial gastrocnemius muscle flap has been described. Lengthening of the sural vascular pedicle was obtained using interposition vein grafts. This allowed coverage of a larger defect than that which could have been obtained with the tethered muscle and without further insult to the already disturbed lower extremity anatomy. The principle of pedicle lengthening can be used to increase the arc of rotation of various other muscle, myocutaneous, skin, or bone flaps and thereby increase their usefulness.
Adult↗
Experimental brain ischemia: neuron-specific enolase level in cerebrospinal fluid as an index of neuronal damage.
Levels of neuron-specific enolase (NSE) were measured in rat CSF following occlusion of the four major arteries to the brain for 10, 20, or 30 min. In the CSF of rats submitted to 30 min of total ischemia, an up to nine-fold increase of NSE level occurred within the first few hours and then slowly diminished. Significant levels were seen for as long as 8 days. Histological observations 3 days after ischemia showed neuronal loss as well as neuronal damage in several forebrain regions such as hippocampus, striatum, and thalamus. Ischemia was followed by transient decreases in exploration behavior and neurological states that were no longer visible 24 h later. After 10 or 20 min ischemia, NSE levels were increased to a lesser degree and fewer damaged neurons were observed. The positive correlation between duration of ischemia and amount of NSE release in CSF indicates that the measurement of NSE in the CSF is a sensitive and reliable index of neuronal lesions.
Animals↗
Planned brief psychotherapy in clinical practice.
A workshop was set up at the Tavistock Clinic to study the application in other clinical settings of techniques of brief psychotherapy developed there. Five people from mixed professional backgrounds met for three years and treated patients, unselected for brief therapy, utilizing these techniques. Of the 15 patients who embarked on treatment, 13 completed it, and 12 of these were followed up for periods ranging from three months to two and a half years. Forms were developed by the workshop to chart the clinical progress of therapy, and to evaluate data on the patient, the psychodynamic focus and treatment process. These data were compared with outcome, rated behaviourally and psychodynamically. At non-significant levels, results indicated that outcome was better for those patients who experienced high levels of distress subjectively but who functioned well externally, those who had a supportive relationship outside therapy and those for whom a psychodynamic focus could be formulated and worked with. Unrelated to outcome were: the extent of early deprivation, severity of damage to object-relations capacities, time of onset and all of the practical aspects of therapy. Clinical illustrations are given and three exceptions to the trend of results are discussed. In general, the findings corroborate Malan's (1976) work. The claim is made that these results justify further exploration of the technique's applicability in NHS out-patient psychotherapy clinics.
Adolescent↗
Determination of 2-[N-[(S)-1-ethoxycarbonyl-3-phenylpropyl]-L-alanyl]-(1S,3S,5S)-2- azabicyclo[3.3.0]octane-3-carboxylic acid (Hoe 498) and its hydrolysis product in serum and urine.
A highly sensitive and specific enzymatic assay for the quantitative determination of 2-[N-[(S)-1-ethoxycarbonyl-3-phenylpropyl]-L-alanyl]-(1S,3S, 5S)-2-azabicyclo[3.3.0]octane-3-carboxylic acid (Hoe 498) and its hydrolysis product Hoe 498-diacid in serum and a GLC method for the simultaneous determination of both compounds in urine are described. Both methods involve extraction from the respective biological fluid using disposable C 18 columns. In serum, the enzyme-inhibiting properties of Hoe 498-diacid (Hoe 498 can readily be converted to its hydrolysis product) are utilized for the determination of both compounds concurrently. The serum extract is dissolved in buffer solution and incubated with human serum as angiotensin converting enzyme (peptidyl-dipeptide-hydrolase, EC 3.4.15.1) source and hip-his-leu substrate. The hippuric acid liberated is quantitated by HPLC. The urine extract is treated with diazomethane followed by trifluoroacetic anhydride to convert Hoe 498 and Hoe 498-diacid to their methylester, trifluoroacetyl derivatives, which are then determined simultaneously by GLC using a nitrogen specific detector.
Angiotensin-Converting Enzyme Inhibitors↗
Thin-layer chromatographic determination of procainamide and N-acetylprocainamide in human serum and urine at single-dose levels.
Thin-layer chromatographic methods were applied for bioavailability studies of procainamide in serum and urine. Detection of the parent compound and the major metabolite was performed in the ultraviolet range at 275 nm. Using 100-microliter samples, detection limits were 60 ng of procainamide-HCl per ml serum and 7 micrograms/ml urine, and 60 ng of N-acetylprocainamide-HCl per ml serum and 5 micrograms/ml urine. Advantages over previous methods are discussed. From serum and urine data of five volunteers, the bioavailability of procainamide from a 250-mg dragee preparation compared with an intravenous dose was verified. Pharmacokinetic data were computed using one-compartment open models. Results corresponded well with values previously published.
Acecainide↗
Thin-layer chromatographic determination of imipramine and desipramine in human plasma and urine at single-dose levels.
Thin-layer chromatographic methods were up-dated for pharmacokinetic studies of imipramine in plasma and urine. The free parent compound and its free desmethyl metabolite desipramine are determined in plasma. Conjugates of both compounds in urine are cleaved on treatment with glucuronidase/arylsulfatase. Following chromatography, intense yellow derivatives are obtained overnight on standing or by exposure to nitrous gases. Detection is performed in the visible range at 405 nm (plasma) or 460 nm (urine). The methods are selective, accurate and sensitive, with detection limits for plasma of 2 ng/ml imipramine--HCl and 2 ng/ml desipramine--HCl, and 0.06 micrograms/ml total imipramine--HCl and 0.126 micrograms/ml total desipramine--HCl for urine. Pharmacokinetic data from plasma and urine results following single oral doses of 50 mg imipramine--HCl to eight volunteers were computed using one-compartment open models.
Adult↗
Developmental changes in translatable mRNAs for the cerebral enolase isozymes alphaalpha and gammagamma.
Using the rabbit reticulocyte cell-free translation system we have estimated during ontogenesis the proportions of in vitro translatable alpha and gamma brain enolase mRNAs, which are two minor mRNA species. No polypeptide precursor to these enzyme subunits appears to be synthesized during translation in vitro. During brain development, the changes in translatable alpha and gamma mRNA content seem to parallel those of the corresponding antigens. The proportion of each of the enolase mRNAs is highest in adult mouse brain. Mechanisms controlling alpha and gamma antigen expression are discussed. In order to prepare the specific cDNA probes, purification of alpha and gamma mRNAs was undertaken.
Animals↗
Pancreatic nesidioblastosis in adults. Report of a patient with hyperinsulinemic hypoglycemia.
Hyperinsulinemic hypoglycemia in neonates occurs most commonly secondary to nesidioblastosis, a persistence of fetal development of the endocrine cells of the pancreas. In adults, hyperinsulinemic hypoglycemia is usually due to insulinoma or beta-cell hyperplasia. Nesidioblastosis arising in adults has only recently been reported. An adult patient with nesidioblastosis has been described. The light and electron microscopic films of this entity are shown in detail, and the embryology and treatment are discussed. Further understanding of this newly described disease entity will require research on factors that regulate the differentiation of beta cells of the pancreas.
Female↗
Changes in the expression of the alpha alpha form of enolase during neuroblastoma differentiation.
The relative amounts of the different enolase isozymes present in neuroblastoma cells change during differentiation. When differentiation is induced by low serum in the presence of DMSO (dimethyl sulfoxide), there is a 50% decrease in the concentration of enolase activity associated with the form alpha alpha, and an increase in the activity associated with the gamma-containing isozymes (alpha gamma plus gamma gamma); in the absence of DMSO, there is no decrease in alpha alpha or in total enolase activity. In order to study the mechanism of the changes in alpha alpha, cells differentiated with low serum with and without DMSO were compared. Measurements of the concentration of the alpha antigen by microcomplement fixation and by immunotitration demonstrate that the decreased enolase activity in DMSO cells is due to a decreased concentration of the alpha antigen. Measurements of the relative rate of synthesis of the antigen show that the decreased concentration of the alpha antigen is due to a decreased rate of synthesis. Enolase in differentiated cells is sufficiently stable (t1/2 greater than 100 h) that a comparison of the relative rates of degradation has not been possible. The decreased synthesis of the alpha subunit of enolase that occurs under these conditions appears to be a useful model system for studying the de-expression of the alpha gene that occurs in vivo during neuronal differentiation.
Animals↗
[Modular system for the total replacement of the femur--Endo-model].
Total femur replacement may offer a useful solution in cases of severe trauma or neoplasia and in revision arthroplasty after failed total joint replacement when gross losses of bone have resulted in an unloadable femur and all other methods of treatment would fail to provide the patient with a stable leg. Until recently, total femur components were custom-made, but now we have a comprehensive modular total femur implant available reducing problems of preoperative preparation and simplifying the manufacture of custom-made designs. The new device can be shaped and adapted to the conditions encountered at the time of operation. The "push-through" type of prosthesis can be mounted step by step and converted into a total femur replacement with either a conventional head or a saddle at the proximal end and a total axial rotating knee prosthesis at the distal end. Elongation of the entire system is feasible.
Adult↗
A new double labeling technique using tyrosine hydroxylase and dopamine-beta-hydroxylase immunohistochemistry: evidence for dopaminergic cells lying in the pons of the beef brain.
Despite numerous data on the organization of the central catecholaminergic (CA) system, the nature of many CA terminal fields, fiber pathways and cell bodies, remains unknown. The discrepancies between the results obtained by CA histofluorescence and dopamine-beta-hydroxylase (DBH) immunofluorescence method suggest that certain areas described as noradrenergic (NA) may be in fact dopaminergic (DA). A method to solve this problem is to compare the localization of the two CA synthetizing enzymes: tyrosine hydroxylase (TH) and DBH. The first enzyme is a marker of all CA neurons, whereas the second one is a specific marker of noradrenergic and adrenergic neurons. We decided to develop a technique which allows visualization of both antigens on a same section. We chose to combine immunofluorescence and immunoperoxidase techniques which yield very different staining. The optimal conditions for this double labeling technique were developed in bovine brain, the homologous system for our antibodies. The results obtained by this procedure provide evidence that DA cells together with NA cells extend throughout the rostral part of the pons, and outline a successful technique for a more extensive investigation of the DA system. The same method should be applicable to other investigations involving other antigens.
Animals↗