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Biomedical subjects

A Keiler

Publications and source records attributed to A Keiler.

At least 37 records · Page 2Linked to original sources
PubMed1979

[Significance of alpha 1-acid glycoprotein for the diagnosis of stomach cancer (author's transl)].

In 41 patients with benignant and malignant diseases of the stomach the appearance of alpha 1-acid glycoprotein (alpha1sGP) in the gastric juice was recorded quantitatively by radial immunodiffusion and compared with normal serum alpha1sGP by immunelectrophoretic analyzation and by immunodiffusion after after Ouchterlony. In 80.0% of the patients with carcinoma, in 11.8% of patients with gastric or duodenal ulcer and in 28.6% of the patients with gastritis the presence of alpha1sGP could be evidenced in the gastric juice. The appearance of this glycoprotein significantly differed in benignant and malignant diseases of the stomach. A polymorphism of the stomach alpha1sGP compared with normal serum alpha1sGP could not be established by the applied methods. Therefore it is unprobable that the gastric alpha1sGP is originated by the malignoma.

Diagnosis, Differential↗
PubMed1979

Endotoxin-induced myeloid reactions in dogs.

The response to artificial endotoxinemia was studied in adult dogs. Granulocyte-macrophage colony stimulating activity (CSA) in lung tissue and blood was measured along with the number of circulating granulocytes and myeloid committed stem cells (colony forming units, CFUc). Acute endotoxinemia induced a measurable CSA increase in lung tissue before being detectable in blood. Granulocytes, rapidly removed from the circulation, showed no release of CSA during sequestration. These experiments demonstrate that the process of endotoxin recognition and subsequent transition into a myelopoietic stimulus is medicated by cells belonging to tissue; mature granulocytes, involved in the defence against bacterial infection, do not release activity that promotes growth of immature myeloid cells.

Animals↗
PubMed1979

In vitro evidence of cellular and humoral immune responses following lung allotransplantation in canine recipients with and without immunosuppressive treatment.

Using donor lung antigens as targets, two test methods, a rosette-forming cell test (RFC) and indirect immunofluorescence test (indir. IF), were applied to focus on the first set lung allograft rejection in canine recipients with and without immunosuppressive treatment. In both groups the first sign of humoral or cellular sensitization became evident on the 4th postoperative day, which might answer some unsolved problems in early lung graft damage, recently discussed as a manifestation of certain immunologic reactions. The tests used, indir. IF and RFC, revealed a steadily climbing tendency of immunization in untreated recipients and more variable and reversible positive results in the immunosuppressively treated group. No correlation between cellular and humoral immunity could be proven by our tests. The fact that we found no incorrect positive results in RFC suggests that this test provides important hints for the diagnosis of lung allograft rejection.

Animals↗
PubMed1979

Donor- and organ-specific evaluation of antibodies eluted from canine lung allografts rejected by immunosuppressively treated and untreated recipients.

Using elution techniques, the humoral lung allograft rejection in immunosuppressively treated versus untreated recipients is analyzed at the donor organ specific level. The antibodies were evaluated quantitatively and qualitatively by elution of lung graft bound immunoglobulins and by testing the eluates against donor lung antigen using passive hemagglutination and indirect immunofluorescence. By correlating the results in those assays, a considerable amount of humoral anti-donor lung antibodies could be proved only in dogs not treated immunosuppressively, and there was no accordance with the results of direct immunofluorescence by quantification. The difference in the organ-bound antibodies between immunosuppressively treated and untreated lung grafts seems to be remarkable because of a similar mononuclear infiltration. Thus, enabling a specific improvement of some previous speculations about the lung-specific humoral alloimmune reaction, this type of rejection seems to be similar to rather stereotypical allograft rejection, but may be modified by a standard immunosuppression with methylprednisolone and azathioprine.

Animals↗
PubMed1979

[The Le Veen peritoneo-jugular shunt and the testing of its function].

A radioisotope technique for testing the patency of, peritoneo-venous shunts of Le Veen, is reported and is demonstrated by discussing a case history. By now a total number of 16 patients have been tested, after implantation of such a shunt. The demonstrated radioisotope technique, which causes no complaints from the patient, is not only able to inform concerning the functional state of the Le Veen shunts, but also, in case of disturbances, is able to reveal the cause of the underlying malfunctions.

Ascites↗
PubMed1979

[The effects of oxprenolol on cardiac function during hypovolemic shock in dogs (author's transl)].

Effects of beta-receptor blockade by oxprenolol, which significantly prevented subendocardial necroses during hemorrhagic shock in dogs, on shock tolerance and myocardial function were analyzed. Overall mortality was not altered by beta-receptor blockade. Cardiac output and contractility (dp/dtmax) before, during and after a hypovolemic period of 120 to 210 min with mean arterial pressure = 40 +/- 5 mmHg showed no significant difference with or without oxprenolol treatment. Increase of heart rate during hemorrhage was abolished completely by oxprenolol and as a consequence of this duration of the diastolic filling period was about three times longer (p less than 0.001) and stroke volumes were greater. Stress metabolism was improved. Hyperglycemia and metabolic acidosis were diminished and arterial oxygen tension was higher in the treated group. Incidence of lethal ventricular fibrillation was higher and pulsus alternans found only in the control group. The beneficial effects of beta-receptor blockage on the course of hemorrhagic shock are explained by the prevention of the catecholamine induced tachycardia and thereby increased coronary perfusion and decreased myocardial oxygen consumption and by the intrinsic sympathicomimetic activity of oxprenolol.

Acidosis↗
PubMed1978

[The problem of anti-lung basal membrane immunity in the framework of allogenic lung transplant rejection].

This study is concerned with the significance of autoimmune processes caused by the damage of lung tissue after lungallo- and autotransplantation. In 15 mongrel dogs a left side lung-allotranplantation was performed, 5 of them were treated with immunsuppressive therapy in the posttransplantation period. A group of 5 dogs with autotransplantation of the left lung served as a control-group. In the posttransplantation period the development of humoral antibodies responding with lung basal membrane antigens was examined by daily taken sera as well as by elution of immuneglobulins from the rejected grafts in the passive hemagglutination and compared with the development of alloantibodies against donor-lung's tissue. Besides of regularly traceable humoral alloantibodies of lung allograft recipients in the posttransplantation period, in no group antilung basal membrane antibodies could be found.

Animals↗
PubMed1977

[Complement system and lymphocytotoxic antibodies in presensitized dogs after homologous lung transplantation ].

Left side orthotopic homotransplantation of the lung was performed in 8 mongrel dogs pre-sensitized by full-thickness skin transplants. The degree of sensitization was assessed by control of the lymphocytotoxic antibody titre. The behaviour of haemolytic complement activity, as well as the alterations in antibody titre were recorded up to 60 minutes after opening of the anastomoses. Evaluation was performed by analyses of significance. A significant decrease in lymphocytotoxic antibody titre (p less than 0.001), as well as a significant diminution in complement activity (p less than 0.01) was observed in blood samples from the pulmonary vein of the graft during the first five minutes following recirculation. This resulted from an interaction of both systems in the immune reaction. The further complement consumption by the graft in contrast to the constancy of the lymphocytotoxic antibody titre in the subsequent post-transplantation period can be explained by antibody-independent complement activation. Vena cava-- pulmonary vein differences in the lymphocytotoxic antibody titre and complement activity are correlated only five minutes after opening of the anastomoses (r = 0.75), which confirms the independence of both systems during later course of the reaction.

Animals↗
PubMed1977

[Bronchospirometric results after single lung homotransplantation in dogs with experimentally induced pulmonary emphysema (author's transl)].

Pulmonary emphysema was induced in 8 bastard dogs by intratracheal instillation of Papain. Development of emphysema was documented by pulmonary function tests. The emphysematous animals underwent single lung homotransplantation. Postoperative bronchospirometric measurements revealed that up to the 6th week after transplantation there is no evidence of serious ventilation perfusion inbalance.

Airway Resistance↗
PubMed1977

[Specific humoral immunity in the circulation of dogs with allotransplanted lungs].

A method appropriate for identification and quantification of circulating antibodies reacting with donorlungantigens is presented in allogenous lungtransplantation. Following incubation of native tissue-slides taken from the second removed but not grafted donor lung with the recipient's serum, the specific humoral antibodies are detected by indirect immunfluorescence. Quantifying evaluation is performed by serum-dilutions. The preexisting specific antibody-titer found in DLA-sensitized animals shows a significant correlation with the duration of graft function when compared with lymphocytotoxic antibody-titer. In unsensitized dogs a regular appearance of circulating specific antibodies can be seen beginning from the fourth or fifth postoperative day. The increasing antibody-titer during further course is a feature of a humoral graft rejection represented in the periphereal blood-circulation.

Animals↗
PubMed1977

[Antibiotic therapy with doxycycline in dogs with lung transplantations (author's transl)].

In contrast with kidney, heart and liver transplantations, lung transplantation involves an organ which has contact with the environment and bears a special risk of infection. This circumstance and the diminished resistance during immunosuppressive therapy makes long-term treatment with an effective broad-spectrum antibiotic advisable for lung transplantations. Taking into consideration the different objectives and questions within the research project concerned the 182 lung allotransplantations carried out in dogs can be divided into two therapeutic groups in regard to postoperative treatment: lung allotransplantations with and without immunosuppressive therapy (methylprednisolone, azathioprine). Long-term doxycycline therapy was carried out in both groups. Those animals which were not treated with immunosuppressive drugs survived for an average of 9 days, those receiving immunosuppressive therapy survived for an average of 30 days. The longest survival period was 2.5 years. Clinically and histologically it could be demonstrated that doxycycline was successful both during the immediate postoperative period and in antibiotic long-term therapy after experimental lung transplantations.

Animals↗
PubMed1976

[Evidence of donoroganspecific humoral antibodies in hyperacutely and acutely rejected canine lungallotransplants (author's transl)].

A method to eluat donororganspecific antibodies enabling direct and specific access to the pattern of humoral rejection after lung allotransplantation is demonstrated. The antibodies were evaluated quantitatively and qualitatively by elution of lung-grafts immunoglobulins and by a consequent investigation of the eluates using the passive haemagglutination- and indirect immunflourescence- test against donors' lung-antigen. Humoral antidonorlung-antibodies could be proved in all rejected grafts belonging to sensitized as well as to unsensitized recipients. There can be seen a highly significant correlation when comparing the results of the passive haemagglutination and indirect immunfluorescence test (r = 0,93, p less than or equal 0,01). On the other hand negative results of the investigations of eluates by lungantigens of other dogs show the specifity of humoral graft rejection. The suprising fact that there does not exist an essential difference between the results of sensitized and nonsensitized animals reveals a humoral immunresponse in hyperacute as well as in acute rejection.

Animals↗