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A Kasprzak

Publications and source records attributed to A Kasprzak.

50 records · Page 3Linked to original sources
PubMed1984

Sex differences in adrenocortical structure and function. XVII. Analysis of adrenal growth rate in maturing male and female rats and hamsters.

The aim of the study was to analyse the rate of adrenal growth of maturing rat and hamster of both sexes in relation to age, body weight and body surface. In the course of postnatal onto-genesis--days 21 to 77 in rats and 21 to 96 in hamsters--all of the estimated relations between body weight and body surface on adrenal size in both sexes of rat and hamster were almost linear. The values of all analysed regression line slopes are higher in female rats when compared to male animals. Male hamsters had higher values of regression line slopes than females. The results of the study suggest higher activity of the adrenal gland in female rat and male hamster when compared with the animals of opposite sex.

Adrenal Glands↗
PubMed1982

Fluorescence depolarization and rotational modes of tyrosine in bovine pancreatic trypsin inhibitor.

The fluorescence of bovine pancreatic trypsin inhibitor (BPTI) is due to one or more of its four tyrosine residues. Observations of the stationary polarization of the fluorescence over a large range of temperatures and viscosities permit the demonstration of at least three modes of tyrosine rotation, and perhaps an ultrafast fourth one. The slowest mode is one of motion of the whole molecule; the second, a much faster motion limited to an amplitude of 11 degrees, is not changed by quenching of the fluorescence through addition of citrate and is therefore ascribed to the motion of internal tyrosines of BPTI. The third mode of motion is faster still; it has an amplitude similar to that of the second and, being sensitive to citrate quenching, is attributed to the rotation of the external tyrosine residue. A residual depolarization corresponding to a rotational amplitude of 22 degrees is deduced by comparison of the polarizations of BPTI and tyrosine dissolved in 80% glycerol-water at -40 degrees C. It is in accord in amplitude with the picosecond tyrosine rotations predicted by Karplus and collaborators from molecular dynamics computer simulations, but it could also originate, in whole or in part, from electronic energy transfer among the tyrosines.

Aprotinin↗
PubMed1982

Sex differences in adrenocortical structure and function. VIII. Karyometric and stereologic studies on adrenals of intact adult male and female hamsters.

Contrary to the rats, adrenals of the intact adult male hamsters are greater than females' ones. The aim of the study was to elucidate the cytologic aspects of these sex-associated differences in adrenal weight. Despite of equal body weight adrenals of male hamsters are markedly heavier then female glands and the same is true for the volume of the glands. Volume of al adrenocortical zones is larger in males than females animals, on the contrary, volume of medulla is greater in female hamster. The volume of zona glomerulosa cell is equal in both sexes while the cells of fasciculata and reticularis zones are greater in male animals. In the both sexes nuclear volume of glomerulosa and fasciculata cells was similar, on the contrary nuclei of zona reticularis cells are markedly larger in males. Total number of adrenocortical cells is higher in male glands as compared to female and this difference depends on the higher number of parenchymal cells in fasciculata and reticularis zones of the male gland. These data indicate that sex-associated difference in adrenal weight of the hamster depends on the differences in size as well as in quantity of adrenocortical cells in male and female glands.

Adrenal Cortex↗
PubMed1982

Sex differences in adrenocortical structure and function. IX. Stereologic studies on the effects of gonadectomy and testosterone or estradiol replacement on adrenal cortex of adult male and female hamsters.

Adrenal glands of adult male hamster are heavier than in female and this sex difference depends primarily on the greater number of adrenocortical cells and their larger size in male gland if compared to female. The aim of the study was to explain the relationship between the sex hormones and the structural of adrenal cortex of male and female hamsters. Orchidectomy lowered the adrenal weight, an effect partially reversed by testosterone. Changes induced by orchidectomy were found mainly in fasciculata and reticularis zones, after testosterone replacement the volume of the former did not differ from control males. Removal of testes lowered the volume of individual zona reticularis cell, after testosterone administration the volume of these cells did not differ from control males. Neither ovariectomy, nor estradiol replacement has a desired effect on the above mentioned parameters in female adrenal cortex. In both sexes gonadectomy lowered the total number of adrenocortical cells and this effect is not restored to normal by 12-days testosterone or estradiol action. Results obtained suggest that the sex dimorphism in the size and structure of hamster adrenal cortex depends on the stimulatory action of testosterone on the gland while estradiol has no distinct effect on parameters studied.

Adrenal Cortex↗
PubMedDate not supplied

Effects of prolonged infusion with endothelin-1 on the function and morphology of rat adrenal cortex.

A week of SC infusion with endothelin-1 (ET-1) (0.2 microgram.kg-1.hr-1) lowered PRA and raised plasma aldosterone (A) concentration in rats. Kalaemia and the plasma levels of ACTH and corticosterone (B) were not affected. Prolonged ET-1 administration caused a notable hypertrophy of zona glomerulosa (ZG) and its parenchymal cells, without inducing any apparent change in zona fasciculata. Stereology showed that ZG cell hypertrophy was mainly due to the increase in the volume of the mitochondrial compartment and to the proliferation of smooth endoplasmic reticulum (i.e., the two organelles in which the enzymes of steroid synthesis are contained). Isolated ZG cells from ET-1-infused animals evidenced a notable enhancement in their basal production of A and B. The secretory responses of ZG cells to the maximal effective concentrations of their three main stimulators (ACTH, angiotensin-II and K+) displayed comparable increases. These findings indicate that ET-1, when chronically administered, is able to specifically enhance the growth and steroidogenic capacity of rat ZG, and suggest that the mechanism underlying this ET-1 effect involves stimulation of the de novo synthesis of both the steroidogenic enzymes and the membrane framework in which they are located.

Adrenal Cortex↗
PubMedDate not supplied

[Anti-elastin antibodies in systemic lupus erythematosus].

Immunological response to elastin-derived peptides may cause tissue damage with subsequent degradation of the elastic fibres. Therefore, an incidence of anti-elastin antibodies in sera of patients with the systemic lupus erythematosus was studied. Sixty sera from 50 patients with systemic lupus erythematosus and 50 healthy subjects were assayed with dot-immunobinding technique. Titre 1:10 was considered diagnostically significant. Anti-elastin antibodies were diagnosed in 19 patients (31%) where as they were absent in the control group. In all cases anti-elastin antibodies were IgG.

Adult↗
PubMedDate not supplied

Sex differences in adrenocortical structure and function. XXVI. Further studies on the differences in the compensatory growth of the adrenal cortex of the male and female hamster after unilateral adrenalectomy.

The relative adrenal weight of the remaining right adrenal gland was significantly increased 5 days after unilateral adrenalectomy in hamsters of both sexes. This effect was associated with an enlargement of the zonae glomerulosa and fasciculata in males and of the zona fasciculata in females. Unilateral adrenalectomy raised the average volume of zona fasciculata cells in male hamsters and that of zona reticularis cells in females. The number of parenchymal cells in all adrenocortical zones remained unchanged by the removal of the contralateral gland; however, when calculating per mg of adrenal or per cell, the nucleotide uptake by adrenal quarters was notably lowered. A marked drop in plasma cortisol concentration was found in unilaterally adrenalectomized male but not in female hamsters. These findings indicate that the main component of the adrenal compensatory growth in hamsters is the hypertrophy and not the hyperplasia of parenchymal cells.

Adrenal Cortex↗
PubMedDate not supplied

Compensatory adrenal growth in dexamethasone treated male and female hamsters.

The aim of the study was to investigate the unilateral adrenalectomy - induced compensatory adrenal growth in dexamethasone treated male and female hamsters (Mesocricetus auratus Waterhouse). Animals were treated for 5 days with 25 micrograms dexamethasone/animal/day or with 0.2 ml 0.9% NaCl. The first injection was made 0.5-1 h after monolateral adrenalectomy or sham operation. In three sets of experiments male and female hamsters received no injection. Only in one of three experiments was the relative adrenal weight of monoadrenalectomised male and female hamsters receiving no injections higher than in sham operated groups. In the case of NaCl treatment, only in one group of monoadrenalectomised males was the relative weight of the right adrenal higher than in the control group. On the contrary, in all three experiments with dexamethasone treatment the relative weight of the solitary adrenal was higher than in sham operated animals, while there was no difference among appropriate groups of females. In monoadrenalectomised dexamethasone-treated male hamsters, volume of the glomerulosa and reticularis zones was higher than in sham operated group. Neither the average cell volume in particular adrenocortical zones nor the number of parenchymal cells in the zones and in the entire cortex were changed due to adrenalectomy in dexamethasone treated males. There was no difference in all stereologic parameters studied when monoadrenalectomised and sham operated dexamethasone treated females were compared. Plasma cortisol level was lower in hemiadrenalectomised dexamethasone-administered males, while 3H-thymidine incorporation was higher in both male and female dexamethasone-treated hemiadrenalectomised hamsters. The obtained results demonstrate the evident sex-dependent response of the hamster adrenal gland to monoadrenalectomy and dexamethasone.(ABSTRACT TRUNCATED AT 250 WORDS)

Adrenal Glands↗