Search PubMed⌕ Search

Biomedical subjects

A Karmen

Publications and source records attributed to A Karmen.

51 records · Page 3Linked to original sources
PubMed1967

Fatty acid transport and incorporation into human erythrocytes in vitro.

When human erythrocytes were incubated in vitro with (14)C-labeled free fatty acids bound to serum albumin, labeled fatty acids were incorporated into erythrocyte triglycerides and phospholipids. The first step in this reaction was the transfer of free fatty acids from the albumin to the cells. This transfer was rapid and reversible. The acids were distributed between albumin and cells according to the relative quantities of albumin and cells present. Each acid had a different distribution coefficient. At equilibrium, relatively larger fractions of the stearic and palmitic acids and smaller fractions of the oleic and linoleic were associated with the cells. All these fatty acids were then slowly incorporated into phospholipids and triglycerides. The rate of incorporation of each was a function of its concentration in the cells, but larger fractions of the oleic and linoleic were incorporated than of the stearic, palmitic, myristic, or lauric. The two processes of transfer and incorporation thus had almost opposite selectivities for the different fatty acids. As a result, the fatty acids incorporated into triglycerides and phospholipids resembled in composition the fatty acids on the albumin except for moderately less stearic acid.

Biological Transport↗
PubMed1967

Gas-liquid chromatography of 2-chloroethyl esters using a detector specific for halogens.

The gas-liquid chromatography of 2-chloroethyl derivatives of short- and long-chain monocarboxylic acids, and of short-chain dicarboxylic acids and aldehydes has been studied with a dual-flame ionization detector that provided simultaneous records of the concentrations of chlorine and of total organic material in the column effluent. The chloroethyl derivatives of dicarboxylic acids and aldehydes could be distinguished from the chloroethyl esters of monocarboxylic acids on the basis of different ratios of responses of the two detectors. Analysis of a mixture of chloroethyl derivatives prepared from a natural product revealed that several compounds that might ordinarily have been identified as unsaturated or branched-chain fatty acid esters on the basis of their retention times had the higher chlorine content characteristic of chloroethyl derivatives of aldehydes or dicarboxylic acids.

Chemical Phenomena↗