Biomedical subjects

A Jung

Publications and source records attributed to A Jung.

At least 73 records · Page 4Linked to original sources

PubMed2000

[Changes of kidney parenchyma in children with nephrolithiasis after ESWL treatment in ultrasonography, power Doppler and thermovision monitoring].

The authors discuss the results ultrasonographic (USG), power Doppler (PD) and thermovision (TV) examination in monitoring changes of kidney parenchyma in 30 children with nephrolithiasis after ESWL treatment. To monitor possible effect of shock wave on the kidney parenchyma the USG, PD and TV examination were done before ESWL treatment as well as, 48 hours and 3 months following the treatment. Parts of kidney parenchyma localized on the way of shock wave to the stone were analysed. Echogenicity of kidney parenchyma was analysed by comparison of echo amplitude in subsequent USG examinations. Parenchymal blood flow by computer analyse was estimated. In TV examination the temperature distribution in the place of skin kidney projection was estimated. Changes in echogenicity of kidney parenchyma and impaired kidney parenchymal blood flow 48 hours after ESWL were found. In TV examination 48 hours after ESWL transient reduction in skin temperature was observed in the place of shock wave transmission. In 3 months after ESWL disturbances in the kidney parenchyma in USG, PD and TV were not observed.

Adolescent↗

PubMed2000

[Results of the treatment of pre-urolithiasis state in children with recurrent urinary tract infections].

Urolithiasis often coexists with recurrent urinary tract infections (RUTI). The aim of the study was to determine the correlation of preurolithiasis state (PS) and recurrent urinary tract infections and to establish an effect of the treatment UTI recurrence incidence. PS was found in 202(21.1%) children, most frequently: hyperoxaluria--in 61/202 (30.2%), hypercalciuria--in 32/202 (15.8%), and hyperuricosuria--in 30/202 (14.9%) children. Complex metabolic abnormality was observed in 62/202 (30.7%) patients. Therapeutic management comprised of: antibacterial prophylaxis, high fluid intake, proper diet, correction of urine pH, and pharmacological treatment if necessary. Disappearance of RUTI and PS in 88/202 (43.6%) children, disappearance of RUTI in spite of persistent PS in 36/202 (17.8%), and decrease of RUTI in 54/202 (26.7%) patients were method. In 110/202 (54.5) children PS disappeared.

Adolescent↗

PubMed2000

[Beta-2 microglobulinuria in children with vesico-ureteral reflux and recurrent urinary tract infections].

Recurrent urinary tract infections in children with vesico-ureteral reflux are the one of risk factors in the process of reflux nephropathy. One of markers of early kidney parenchyma damage is beta 2-microglobulin. The aim of the study was to evaluate the value of beta 2-microglobulin excretion in urine and its serum levels in children with vesico-ureteral reflux and recurrent urinary tract infections. It was found that abnormal urinary excretion of beta 2-microglobulin and its serum levels of proceeded post-inflammatory changes in kidney parenchyma observed in imaging examinations of urinary tract and impared parameters of renal function in biochemical analyses.

Adolescent↗

PubMed2000

[Clinical assessment of Uro-Vaxom in the treatment and prophylaxis of recurrent urinary tract infection in children: preliminary results].

The aim of the study was to determine the efficiency of Uro-Vaxom in the treatment of recurrent urinary tract infection in children. We examined 19 girls in aged 4-17 years treated in our Department since Jan 1998 until Jan 1999 for of recurrent urinary tract infection induced by E. coli (RUTI). All girls have been cured with Uro-Vaxom in single daily dose for 3 months. Disappearance of RUTI in 47% of children and decrease in RUTI in 42% reveals that Uro-Vaxom plays significant role in the treatment of this disease.

Adjuvants, Immunologic↗

PubMed2000

[Specificity of uroflowmetry as a screening test for the urinary tract diseases in children].

The aim of the study was to determine the specificity of uroflowmetry a screening testing the urinary diseases in children. We have investigated 70 children. Patients were divided in 3 groups. Group I included 38 children with recurrent urinary tract infections, group II included 18 children with enuresis, and group III included 14 children with preurolithiasis state. Pathological uroflowmetry was detected in 11 children (15.7%), which indicating the need of using this test during diagnostic process.

Adolescent↗

PubMed2000

[The effect of treatment methods on frequency of relapse in nephrotic syndrome in children].

The purpose of the work was to evaluate the influence of the treatment method in nephrotic syndrome on remission time and frequency of sickness relapse. Assessments were made in 26 children aged 1-17 years hospitalised in the Pediatric and Nephrology Department of the Military School of Medicine from 1993-1999. All children were steroid-sensitive, but only one patient didn't have relapse during the 3-years of observation. Because the most of children had oedema syndrome (92.3%) the treatment was started with intravenous hydrocortisone and continued with oral prednisone according to International Study of Kidney Disease in Children (ISKDC) criteria. 10 children with steroid-dependent syndrome were treated with intravenous methylprednisolone. Levamisole was given to 7 patients with concomitant respiratory infection. 7 other children were qualified for chlorambucil treatment. Choice of the treatment method in steroid-dependent and frequently relapsing nephrotic syndrome should be concordant with specificity of the particular patient's disease.

Administration, Oral↗

PubMed2000

[Analysis of erythrocyturia causes in children].

The aim of this study was to assess, on the basing on clinical observation, the causes of erythrocyturia in children. The study include 438 children (214 girls and 224 boys) between 6 month and 17-teen years old with erythrocyturia, treated in Pediatric Nephrology Department from September 1992 till October 1999. The most common was the group of children with urolithiasis--162 (36.99%) and preurolithiasis state--126 (28.77%). In 153 cases urolithiasis was the only reason of erythrocyturia and in 9 children near urolithiasis the other reason (vesicouretheral reflux, urinary tract infection, glomerulitis, polycystic kidney) has been found. In 103 children the preulithiasis state was the only cause of erythrocyturia, in 23 children it was coexisted with others (vesicouretheral reflux, urinary tract infection, glomerulitis). As the more rare common causes were established vesicouretheral reflux, urinary tract infection, glomerulonephritis, in 36 children (8%) we did not find the reason of erythrocyturia. Variety of the reasons makes differential diagnostics of erythrocyturia complicated and needs experience and specialistic diagnostic investigation.

Adolescent↗

PubMed2000

[Prevention of influenza--current recommendations].

Recommendation of the control of influenza include principal changes as follow: the age for universal vaccination has been lowered to 50 years from 65 years scheduling of large, organized vaccination campaigns after mid-October--2000/2001 trivalent vaccine virus strains are A/Moscow/10/99 (H3N2)-like, A/New Caledonia/20/99 (H1N1)-like, and B/Beijing/184/93-like strains.

Adolescent↗

PubMed2000

[beta-Catenin induces invasive growth by activating matrix metalloproteinases in colorectal carcinoma].

beta-catenin was shown to be a major oncoprotein in colon cancer development. Its oncogenic function as a transcriptional activator is upregulated by mutations in the APC tumor suppressor gene, leading to a constitutive activation of the proliferation-associated genes c-myc and cyclin D. The aim of this study was to demonstrate a role of APC-mutations and dysregulated beta-catenin also for the progression of colorectal cancer, by identifying new target genes of beta-catenin associated with tumor invasion and metastasis. Potential invasion genes regulated by beta-catenin and its DNA binding partner TCF4 were identified by a computer search for the consensus DNA binding sequence in relevant promoter regions. Specific DNA binding was confirmed by gel shift assays. Functional importance of beta-catenin for the activation of identified genes was determined by luciferase reporter assays. The significance was demonstrated by coexpression of nuclear beta-catenin and the identified target genes by immunohistochemistry. Among other invasion genes, we identified the matrix metallo proteinases MMP-7 and MMP-1 activated by beta-catenin in the tumor cells. MMP-7 is an important factor for invasion and metastasis and overexpressed in 75% of colon carcinomas. The significance for human colon cancer development was demonstrated by a correlated overexpression of beta-catenin and the MMPs, beginning in large, severely dysplastic adenomas. Our results explain the high percentage of MMP-7 overexpression in colorectal tumors and the resulting activation of invasive growth. Moreover by identifying dysregulated beta-catenin as a transcriptional activator of MMPs and other invasion factors, we demonstrated an important role of mutated APC not only for early steps but also for the progression of colorectal carcinogenesis.

Cadherins↗

PubMed1999

beta-catenin regulates the expression of the matrix metalloproteinase-7 in human colorectal cancer.

Most colorectal cancers have loss of function mutations in the adenomatosis polyposis coli (APC) tumor suppressor gene. This leads to accumulation of beta-catenin, which together with the DNA binding protein TCF-4 functions as a transcriptional activator. Recently defined target genes are c-myc and cyclin D1, linking the APC gene defect to the capacity for autonomous proliferation of colon tumors. Here we report the identification of the matrix metalloproteinase MMP-7 as another target gene of beta-catenin/TCF-4. MMP-7 is overexpressed in 80% of human colorectal cancers and known to be an important factor for early tumor growth, with a potential function also for later progression steps, like invasion and metastasis. Our results explain the high percentage of MMP-7 overexpression in colon tumors. Moreover they indicate that defects in the APC tumor suppressor gene may also have an influence on later steps of colon tumor progression.

Biomarkers, Tumor↗

PubMed1999

Negative regulation of CD4 expression in T cells by the transcriptional repressor ZEB.

ZEB, an E-box binding transcriptional repressor, is an important regulator of T cell and muscle development. Targeted disruption of ZEB in mice resulted in a strong reduction of thymocytes and the few T cells that reached the mature stage were predominantly CD4(+). CD4 expression during the various stages of T cell differentiation is controlled at the transcriptional level by a complex array of regulatory elements in the CD4 gene locus, consisting of at least three enhancers, one promoter and one silencer. Here we present evidence that CD4 gene expression is negatively regulated by ZEB. We show that ZEB binds to the 5'E-box in the CD4-3 element of the proximal CD4 enhancer in competition with the transcriptional activators E12 and HEB, thereby reducing CD4 expression on CD4 single-positive but not CD4/CD8 double-positive T cells. The conversion of the CD4 proximal enhancer into a potential silencer element by the transcriptional repressor ZEB offers an additional concept of CD4 gene regulation in T cells.

Animals↗

PubMed1999

Unexplained fever-analysis of 233 cases in a referral hospital.

A study was conducted to analyse the causes of fever of unknown origin (FUO) in a teaching hospital in central India. Study subjects consisted of 233 patients having FUO admitted in the medical ward. Specific causes of FUO were identified in 73.4% cases. The commonest causes (46.4%) were of infectious diseases origin foremost being enteric fever (29.6%) followed by malaria (9.0%) and tuberculous fever (5.2%). Chloroquine responsive fever accounted for 26% cases of FUO. Enteric fever were seen more commonly in younger adults less than 50 years, tuberculous fever presented usually after four weeks of onset of symptoms and more in elderly patients aged 50 years or more. Intermittent type of fever was more commonly recorded in infectious diseases. Approach to causes of FUO should be focused primarily on infectious diseases followed by other specific investigations. Empirical treatment of cases having intermittent fever with chloroquine seems justifiable even in absence of malarial parasite in peripheral blood smear.

Adult↗

PubMed1998

Potentiation of D2-dopamine receptor-mediated suppression of zif 268 by non-competitive NMDA receptor antagonists in reserpinized rats.

Striatopallidal output neurons, which coexpress D2-dopamine receptors and NMDA receptors, are logically a potential site of interaction between corticostriatal glutamatergic input and dopaminergic systems. Recent hypotheses about the etiology of schizophrenia have implicated both excitatory amino acid and dopamine systems. The present study was designed to examine, in vivo, the interaction between D2-dopamine receptors and NMDA receptors in the regulation of the expression of the early immediate genes (IEGs), zif 268 and jun B, in striatopallidal neurons. We tested whether coadministration of NMDA antagonists interacted with the actions of the D2 agonist, quinpirole, on IEG expression following dopamine depletion with reserpine. When rats were pretreated with the non-competitive NMDA receptor antagonists, MK 801 (1 mg/kg) or PCP (20 mg/kg), together with quinpirole, the quinpirole reversal of reserpine induction of zif 268 mRNA was potentiated in all regions examined. MK 801 alone had no significant effect on reserpine induction of zif 268 mRNA. Pretreatment with the competitive NMDA receptor antagonist, CPP (5 mg/kg), did not significantly alter the dose response of zif 268 mRNA expression to quinpirole in any region. There was no significant effect of MK 801 on jun B mRNA expression, either on the response to quinpirole or when administered alone with reserpine. Our findings provide evidence of an interaction between the NMDA receptor channel system and the D2-dopamine system on a molecular level in striatopallidal neurons carrying output from the basal ganglia.

Animals↗

PubMed1998

[Arthritis as first symptom of leukemia in children].

Three children out of 30 (10%) referred for juvenile chronic arthritis had leukaemia. The patients had complained of intermittent musculoskeletal pain and painful joint swelling for three weeks, nine months and eighteen months prior to admission. On admission two of the patients had active arthritis with soft tissue swelling in one and three joints respectively. The third patient had only arthralgias and no joint swelling. All patients had slight anaemia, normal to slightly reduced thrombocyte count, slight neutropenia and absence of blasts in the peripheral blood. The correct diagnosis was made by bone marrow aspiration. Two children had acute lymphoblastic leukaemia and the third acute myeloblastic leukaemia. Leukaemia thus remains an important differential diagnosis in children presenting with musculoskeletal pain and/or arthritis.

Arthritis, Juvenile↗

PubMed1998

Predictive value of nuclear beta-catenin expression for the occurrence of distant metastases in rectal cancer.

PURPOSE: Adenomatous polyposis coli protein, glycogen synthetase kinase-3-beta, T cell transcription factor/lymphoid enhancer-binding factor, and beta-catenin modulate cell differentiation and proliferation via the expression of effector genes. It has recently been postulated that beta-catenin is a potent oncogene of sporadic colorectal carcinogenesis and a prognostic tumor marker. Our aim was to investigate whether the nuclear overexpression of beta-catenin, possibly caused by mutations in exon 3 of beta-catenin (CTNNB1), is correlated with distant metastatic spread or disease-free survival in rectal carcinoma. METHODS: Immunohistochemical analysis was performed with an anti-beta-catenin-monoclonal antibody on paraffin sections of two groups of patients (n = 2 x 77) with rectal carcinoma curatively treated by surgery alone. The patients selected were all free of local disease, to exclude surgical influence. Patient groups were matched for age, gender, International Union Against Cancer stage, and year of operation (1982 to 1991) and differed only in subsequent metachronous distant metastatic spread. Follow-up was prospective (median, 9.6 years). Three staining patterns were defined: membranous (normal), diffuse cytoplasmic (pathologic), and intense nuclear staining (pathologic). When intense nuclear staining was defined, the specimen was microdissected. Then, DNA was isolated, polymerase chain reaction-amplified, and sequenced to detect mutations in exon 3. RESULTS: Nuclear overexpression of beta-catenin correlated neither with distant metastatic spread (chi-squared, 0.37; P = 0.79) nor with disease-free survival (log-rank with trend, P = 0.62). No mutations were found in the area of the serine/threonine-kinase glycogen synthetase kinase-3-beta-phosphorylation site in exon 3 (CTNNB1) of beta-catenin. CONCLUSION: Although beta-catenin seems to play an important role in early colorectal carcinogenesis, its value as a prognostic marker is questionable. It must be assumed that metastatic ability is determined by other factors than the disturbance of the beta-catenin T cell transcription factor/lymphoid enhancer-binding factor cascade and that other mechanisms might cause the observed nuclear translocation of beta-catenin.

Adenocarcinoma↗

PubMed1998

Apoptosis in chronic gastritis and its correlation with antigastric autoantibodies.

In the course of time, chronic gastritis may result in gastric atrophy, as in type A gastritis, where autoimmune reactions against parietal cells result in a loss of corpus glands. Two antigastric autoantibodies have been detected in Helicobacter pylori gastritis and are described as anti-luminal and anti-canalicular autoantibodies. The aim of this study was to determine whether increased apoptosis may be responsible for the loss of gastric epithelium and whether this apoptosis is correlated with antigastric autoimmunity. Gastric biopsies from normal mucosa and Helicobacter pylori gastritis were analysed for the presence of apoptosis using the TUNEL method. Helicobacter pylori gastritis was divided into cases (1) without autoantibodies, (2) with anti-luminal, and (3) with anti-canalicular autoantibodies. Apoptotic cells of the foveolar and of the glandular epithelium in the antrum and corpus were counted. The number of apoptotic cells in the gastric mucosa was significantly increased in all cases of gastritis. The highest number of apoptotic cells was observed in the gastric glands of the corpus mucosa in Helicobacter pylori gastritis with anti-canalicular autoantibodies. Apoptosis contributes to the development of gastric atrophy and there are various types of Helicobacter pylori gastritis. The positive correlation between apoptotic cell loss in the glandular zone of the corpus mucosa and the presence of anti-canalicular autoantibodies indicates a possible link between anti-gastric autoimmunity and atrophy in this type of Helicobacter pylori gastritis--similar to that in classic type A gastritis.

Adult↗

PubMed1998

Nuclear overexpression of the oncoprotein beta-catenin in colorectal cancer is localized predominantly at the invasion front.

Sixty to eighty percent of all colorectal cancers are characterized by mutations in the APC tumor suppressor gene. Recently, it was shown that these mutations lead to a nuclear overexpression of beta-Catenin by disruption of the wingless/WNT signal pathway. Since nuclear beta-Catenin functions as a transcriptional activator of hitherto unknown tumor genes, this form of beta-Catenin is now considered a major oncoprotein in colorectal cancer. Using immunohistochemistry, we investigated the distribution of overexpressed beta-Catenin within individual colorectal carcinomas. In the majority of the tumors, we found no homogeneous staining, but a strong nuclear expression of beta-Catenin predominantly localized at the invasion front with strongest nuclear staining of isolated, scattered tumor cells. In contrast, cells in the tumor center often showed no nuclear staining, but retained a membranous expression of beta-Catenin, comparable to normal colon epithelium. It is, therefore, likely that in addition to the overexpression of beta-Catenin caused by defects in the APC locus, regulatory events in the tumor itself lead to a different distribution of this oncoprotein. Possibly, surrounding tissue at the invasion front can give signals to the tumor cells, leading to a nuclear translocation of beta-Catenin, where it may play a direct role in tumor invasion processes.

Cell Nucleus↗

PubMed1998

[Resection and replacement of the cervical esophagus and hypopharynx--an interdisciplinary responsibility for visceral, micro- and ENT surgeons].

From 1984 to 1996, 136 carcinomas of the esophagus and 8 of the hypopharynx were resected using 3 different procedures (94 transmediastinal, 36 transthoracic, 14 cervicoabdominal). The hospital mortality rate for cervicoabdominal resection (0%) is unequivocally lower than that of the transmediastinal (17.1%) or transthoracic (14.3%) methods. The 5-year survival rates are not significantly different (24%, 22%, 17%).

Adult↗