Search PubMed⌕ Search

Biomedical subjects

A Jedrzejewska

Publications and source records attributed to A Jedrzejewska.

At least 19 recordsLinked to original sources
PubMed2000

[Effect of asymmetric respiratory exercise therapy on respiratory system function; evaluation using spirometric examination in children with idiopathic scoliosis].

Current work presents the results of spirometric examinations in 124 children aged 5 to 16 years (mean age 12.1 years) suffering from idiopathic scoliosis. Children were treated according to asymmetric respiratory exercises method applied in period of 24 days. Healthy children living in Upper Silesia industrial region were the control group. Examined scoliotic group was characterized by generally mild lung function impairment, although the values of spirometric indexes tended to deplete with time of duration and severity of the scoliosis. Especially the tendency of the forced expiratory volume in first second (FEV1) decrease was apparent, as well as maximal expiratory flows MEF50 and MEF25, in conjunction with Cobb angle increase. Slight but evident increase of forced vital capacity (FVC) and FEV1 was observed as a result of rehabilitation utilizing asymmetric respiratory exercises method.

Adolescent↗
PubMed1994

Ethanol-induced changes in Purkinje cells of the rat cerebellum. I. The ultrastructural changes following chronic ethanol intoxication (qualitative study).

The ultrastructural changes in the Purkinje cells from rat cerebellum following 3 months' ethanol treatment were investigated. The changes in both smooth (SER) and rough (RER) endoplasmic reticulum, mitochondria, microtubules lipofuscin granules of the Purkinje cells perikarya and of their dendrites were evaluated. The prominent dilatations were found in the hypolemmal cisternae as well as in SER canals. The canals in the lamellar bodies, which are a form of RER, were decreased in number, and sometimes they were disintegrated and dispersed throughout the cytoplasm of both perikarya and dendrites as single distended cisternae. The microtubules within dendrites were often disorderly oriented. The increased amount of lipofuscin granules in the Purkinje cells perikarya were also noticed. Our results suggest that chronic ethanol intoxication lead to disturbances in the synthetic capacity of the Purkinje cells, since the ultrastructure of both SER and RER was greatly changed.

Animals↗
PubMed1994

Ethanol-induced changes in Purkinje cells of rat cerebellum. II. The ultrastructural changes after chronic ethanol intoxication. (Morphometric evaluation).

The morphometric approach to evaluation of some organelles of the rat cerebellar Purkinje cells after chronic ethanol intoxication according to the previously elaborated model (Jedrzejewska et al. 1990) was employed. In the experimental rats volumes of both the perikarya and nuclei in the Purkinje cells significantly increased. The quantitative, morphometric changes of both rough and smooth endoplasmic reticulum demonstrated, that in the perikarya and dendrites of the Purkinje cells the volume of rough endoplasmic reticulum decreased and the volume of smooth endoplasmic reticulum increased. The number of lipofuscin granules in the perikarya increased whereas the counts of microtubules per unit area in dendrites decreased. The statistical evaluation of parameters describing mitochondria and Golgi apparatus in ethanol-intoxicated animals did not show significant differences as compared with the control ones.

Animals↗
PubMed1990

Simultaneous labeling of vagal innervation of the gut and afferent projections from the visceral forebrain with dil injected into the dorsal vagal complex in the rat.

The vagal innervation of the different layers of the rat gastrointestinal wall was identified with the fluorescent carbocyanine dye Dil, injected into the dorsal motor nucleus of the vagus (dmnX). Multiple, bilateral injections were used to label all dmnX preganglionic motoneurons, and as a consequence, most of the vagal primary afferents that terminate in the adjacent nucleus of the solitary tract (nts) were also retrogradely and transganglionically labeled. With Fluorogold used to label the enteric nervous system completely and specifically, the Dil-labeled vagal profiles could be visualized and quantified in their anatomical relation to the neurons of the myenteric and submucous ganglia. In the myenteric plexus, vagal fibers and terminals were found throughout the gastrointestinal tract as far caudal as the descending colon, but there was a general decreasing proximodistal gradient in the density of vagal innervation. All parts of the gastric myenteric plexus (fundus, corpus, antrum), as well as the proximal duodenum, were extremely densely innervated, with vagal fibers and terminals in virtually every ganglion and connective. Further caudally, both the percentage of innervated myenteric ganglia and the average density of label within the ganglia rapidly decreased, with the exception of the cecum and proximal colon, where up to 65% of the ganglia were innervated. In the gastric and duodenal submucosa very few and in the mucosa no vagal fibers and terminals were found. With both normal epifluorescence and laser scanning confocal microscopy, highly varicose or beaded terminal structures of various size and geometry could be identified. The Dil injections, which impregnated the dmnX as well as the adjacent nts, resulted in retrograde and anterograde labeling of all the previously reported forebrain connections with the dorsal vagal complex. We conclude that the myenteric plexus is the primary target of vagal innervation throughout the gastrointestinal tract, and that its innervation is more complete than previously assumed. In contrast, vagal afferent (and efferent) innervation of mucosa and submucosa seems conspicuously sparse or absent. Furthermore, the use of more focal injections of Dil offers the prospect to simultaneously identify specific subsets of vagal preganglionics and their central nervous inputs.

Animals↗
PubMed1990

[Histology of the central nervous system in rats after intensive chronic ethanol intoxication].

The influence of intensive chronic intoxication with ethanol on adult Wistar rats was investigated. The animals received through a stomach pump 5 ml of 40% ethyl alcohol solution daily for three months. Structural lesions of the CNS observed in the light microscope consisted of acute and chronic edematous changes with infiltration of mononuclear cells and presence of lymphocytic-microglial nodules and myelin discoloration. Sclerotization of nerve cells with reactive gliosis was also noted in the CA1 sector of ventral hippocampus. The pattern of changes is comparable with the most frequently observed pathological abnormalities observed in the CNS of humans after alcohol abuse.

Alcoholism↗
PubMed1990

Intrastriatal grafts of adrenal medulla in hemiparkinsonian rats--ultrastructural study.

The aim of the study was to investigate the ultrastructure of the right striatum after intrastriatal adrenal medulla grafts in Wistar rats with a 6-OHDA unilateral lesion of the compact part of the right substantia nigra (SN). 12 adult rats were investigated. Two rats were intact, 2 received a sham SN-lesion. Ungerstedt's rotational test confirmed a significant lateral SN-lesion in all the animals. Two weeks after the SN lesion small samples of the adrenal medulla of 2-month old Wistar rats were prepared (0.5 mm3) and implanted stereotaxically into the middle-paraventricular region of the right striatum. The animals were perfused with 2.5% glutaraldehyd according to the following patern: 2--after 1 week of survival, 2--after 3 weeks, 2--after 6 weeks, and 2--after 3 months. 10 samples of ca 1 mm3 were taken from 3 regions of the right striatum (1) the region of the graft, (2) the region in the neighbourhood of the graft, (3) tissue at a long distance from the transplant. Macroscopic observation showed granulomatous-like tissue at the place of the implantation of the graft after 1 week of survival. After 3 weeks and later only the evacuated cavity was observed instead of the graft. A routine electron microscopic procedure was used to expose the material in a JEM 100 B electron microscope. The study of the ultrastructure indicated many leucocytes and microglia cells in the region of the graft as well as features of destruction of the adrenal medulla cells in the rats perfused 1 and 3 weeks after transplantation.(ABSTRACT TRUNCATED AT 250 WORDS)

Adrenal Medulla↗
PubMed1990

Neuropathology of intramural autonomic innervation of mice stomach in chronic ethanol intoxication.

Intramural stomach innervation was investigated in DBA mice after 1 week, 6 weeks and 3 months of intragastric ethanol treatment (1 cm3 daily by plastic tube). The results indicated that the intramural nervous system of the stomach underwent ultrastructural and histological changes in relation to ethanol. Alterations were noticeable after 3 weeks of treatment. They were more strongly pronounced after 6 weeks and were maximally advanced in the group with 3 months of ethanol treatment. Mitochondria seemed to be first affected, especially in the axonal processes and nerve endings (higher density, hypertrophy, inverted pattern of cristae). Dense bodies with double membrane as well as altered fibrocytes and myocytes were noticed after longer periods of intoxication. Ischemic disease of neurons developed after 3 months of ethanol administration, probably against the background of severe mitochondrial damage. Some features suggested reconstruction of the cell population in intramural ganglia.

Alcoholism↗
PubMed1990

Ultrastructure of ventral hippocampus of rat with chronic ethanol intoxication.

Ultrastructural analysis was made of the ventral hippocampus in 5 Wistar rats experimentally subjected to chronic ethanol treatment (daily doses of 5 cm3 of 40% ethanol for 3 months), with a view to identifying morphological features resembling hippocampal damage in human alcoholics. Our results indicated damage of nerve cells in the investigated area, accompanied by glial reaction. The nerve cells were impaired by energetic depletion of the mitochondrial system which, in our opinion, led to pictures analogous to ischemic cell affection and, finally, to cell death. Also, features of premature involution were noted in both pyramidal and granular cells. There were signs of glial hypertrophy, glial cell divisions, lipopigment accumulation, and glial adhesion of affected neurons. We concluded that severe chronic ethanol intoxication affected first of all the mitochondrial apparatus of neurons, leading to more advanced lesion of cells and to pronounced glial reaction.

Alcoholism↗