Search PubMed⌕ Search

Biomedical subjects

A Jakobsen

Publications and source records attributed to A Jakobsen.

At least 127 records · Page 7Linked to original sources
PubMed1984

Ploidy level of human breast carcinoma. Relation to histopathologic features and hormone receptor content.

The ploidy level was investigated by flow cytometric analysis in 143 cases of invasive intraductal mammary carcinoma. Aneuploidy was found in 70% of the tumours. Comparison of ploidy level with histopathologic features, hormone receptor status and clinical characteristics indicated that aneuploid tumours were mostly poorly differentiated (grade III) and oestrogen receptor negative (p less than 0.05). However, this applied only to postmenopausal patients. No correlation appeared between the progesterone receptor status and the ploidy level, but this parameter tended to predict the frequency of lymph node metastases. The possible prognostic significance of these findings is discussed.

Axilla↗
PubMed1983

The use of trout erythrocytes and human lymphocytes for standardization in flow cytometry.

A method of standardization of flow cytometric ploidy measurements using trout erythrocytes and human lymphocytes is described. The sources of errors of the ratio between the modal channel number of erythrocytes and lymphocytes were investigated. The sample standard deviation was 0.3%, and the variation between persons of the same sex was approximately 0.5%. A distinct difference (1.82%) between the two sexes indicated that small deviations of DNA content can be detected. The standardization method was applied to the analysis of biopsy specimens from bladder tumors, normal bladder mucosa, and lymphocytes from the same patients. In diploid populations the standard deviation of the DNA indices was 1.5-2% for normal bladder mucosa but approximately 2.5% for tumors. These values indicate that deviations amounting to 4-5% from the diploidy are detectable by a single analysis of one sample.

Animals↗
PubMed1983

Flow cytometric classification of biopsy specimens from cervical intraepithelial neoplasia.

The distribution of single-cell DNA content was investigated in biopsy specimens from the human cervix of 121 women suspected of having intraepithelial neoplasia. Comparison of the results of the histopathological examination with the ploidy level showed that all normal specimens were diploid. Thus, no false-positive results occurred. Most of the specimens classified as mild and moderate dysplasia were diploid as well. Aneuploid cell populations occurred in 78% of the lesions classified as severe dysplasia and carcinoma in situ. The ploidy level distribution permitted a natural division of the aneuploid cell populations into two groups with DNA indices either above or below 1.5. The importance of the aneuploidy in carcinogenesis is discussed.

Aneuploidy↗
PubMed1983

Characterization of ploidy level in bladder tumors and selected site specimens by flow cytometry.

The histopathological grade of atypia was compared with the ploidy level in biopsy specimens from bladder tumors and six grossly normal areas (selected sites). The study included investigation of 303 specimens from 45 patients. The results demonstrated a high correlation between the grade of atypia and the occurrence of aneuploid cell populations. The selected site specimens were diploid in patients with low grade malignant tumors, while high grade malignant tumors were associated with aneuploid selected site specimens in 20% of the cases. The divergence of ploidy level in the tumor and selected site specimens found in some cases clearly demonstrated that the carcinogenic process in the bladder may lead to clonal heterogeneity. Flow cytometric analysis seems suited for mapping of the ploidy levels in different parts of the bladder. The possible importance of the additional information is discussed.

Aneuploidy↗
PubMed1983

DNA distribution and chromosome number in human cervical carcinoma.

The degree of ploidy of 15 human cervical carcinomas was investigated by use of flow cytometry and cytogenetic analysis. All tumors contained aneuploid cell populations. The results showed no correlation between ploidy and the clinical stage of the tumor. A high correlation was found between modal DNA content and modal chromosome number of the aneuploid cell populations, and it is concluded that flow cytometric analyses reflect the occurrence of tumor stemlines.

Aneuploidy↗
PubMed1981

Influence of HLA matching in cadaveric renal transplantation: experience from one Scandiatransplant center.

The outcome of 461 prospectively HLA-A, -B and -C typed and 193 prospectively HLA-DR typed cadaveric kidney transplants in one center was followed. We found a significant beneficial effect on graft survival both of HLA-A and -B as well as of HLA-DR matching between donor and recipient, while no effects of HLA-C compatibility could be detected. The effect of HLA-DR matching was clearly more pronounced than that of HLA-A and -B matching, and a possible influence of matching for HLA-A and -B could only be seen in the HLA-DR mismatched combinations. Pretransplant blood transfusions were associated with an increased graft survival only in patients receiving HLA-DR mismatched transplants. We conclude that major emphasis should be laid on obtaining HLA-DR compatibility in clinical renal transplantation.

Blood Grouping and Crossmatching↗
PubMed1981

A controlled trial with AHLG Behring: lack of effect on cadaveric renal graft survival.

To assess the effect of Pressimmune (AHLG-Behring), in high doses, (30 mg/kg/bdw for 21 days) as an adjuvant immunosuppressive drug a prospective, randomized controlled trial was carried out in 60 recipients of first time cadaveric renal allografts. There was no improvement in graft survival in the treatment group, and there was no steroid sparing. In the patients receiving Pressimmune, the T-lymphocyte count fell to about half pretransplant levels, and the first rejection episode occurred later than in the control patients, but eventually equally many kidneys were lost from rejection.

Adolescent↗
PubMed1981

Effect of metronidazole and ampicillin on patients with gangrenous or perforated appendicitis. A comparative study.

A study comparing the effectiveness of metronidazole and ampicillin in preventing wound infection in gangrenous or perforated appendicitis without general peritonitis was performed. Metronidazole was given as suppository immediately after the operation (1 g) and the administration continued for 10 days. Initially, one suppository was given every 8 h for one or two days, thereafter one tablet (400 mg) every 8 h until 10 days after the operation. In the ampicillin group, ampicillin (4.0-6.0 g) was deposited intraperitoneally during surgery, followed by 1.0 g ampicillin every 6 h, first intravenously (or intramuscularly) for one or two days and thereafter as tablets. Of the 50 patients initially present in the study, 7 were excluded, 22 received ampicillin and 21 metronidazole. In the first group, 8 patients had perforated, and 14 patients gangrenous appendicitis. In the metronidazole group, the corresponding figures were 11 and 10. The mean hospitalization period after operation was 6.8 days for both groups. In the metronidazole group, wound infection was not observed. In the ampicillin group, 2 patients (9.1%) had wound infections.

Adolescent↗
PubMed1980

Importance of HLA-DR matching in cadaveric renal transplantation: a prospective one-center study of 170 transplants.

To investigate the influence of matching for HLA-DR antigens in renal transplantation, we assessed the outcome of 170 prospectively HLA-typed cadaveric kidney transplantations performed since 1977 in one center. We found a beneficial effect on graft survival of HLA-DR compatibility between donor and recipient (P < 0.05). A possible effect of matching for the HLA-A and B antigens could be seen only in the HLA-DR-mismatched combinations. Pretransplantation blood transfusions were associated with increased graft survival only in patients receiving HLA-DR mismatched transplants (P < 0.02). We conclude that major emphasis should be placed on obtaining HLA-DR compatibility renal transplantation. (N Engl J Med. 1980; 303:850-4).

Blood Transfusion↗
PubMed1980

Renal transplantation in polycystic renal disease--a joint Scandinavian report.

During the period 1965-1977, a total of 339 patients with polycystic renal disease received at least 1 renal transplant at one of 10 transplant centres in Scandinavia. Patient survival at one year was 67%. The one year graft survival of 319 cadaveric grafts was 40%. The average age of the patient was 56.7 years. Patients who were 60 years or older (93 patients) had a significantly poorer patient and graft survival at one year (50% and 29.5% respectively). Patients receiving kidneys with O incompatibilities did significantly better than other donor-recipient combinations. Previous blood transfusions were associated with better graft prognosis, though the difference was only significant for 2 years. The incidence of posttransplant urinary tract infection (present in 47% of all the patients) was twice as common in patients with a history of pretransplant urinary tract infection (seen in 41% of all the patients). There was no association between posttransplant septicaemia and either pre- or post-transplant urinary tract infection. Only 10.5% of the patients were nephrectomized at the time of transplantation, half of these had urinary tract infection. Twenty-four per cent of the patients were nephrectomized in the posttransplant period, half of these because of infection. There was no difference in the graft survival data of the patients with or without pretransplant urinary tract infection. These findings justify a restrictive practice with regard to pretransplant nephrectomy in patients with polycystic renal disease.

Blood Transfusion↗
PubMed1979

Flow cytometric investigations of human bladder carcinoma compared to histological classification.

The DNA distribution has been investigated in biopsies from human bladder carcinomas by means of flow cytometry (FCM). The FCM analysis was based on a preparation method yielding a suspension of single cell nuclei. Biopsies from 45 patients were analysed. The results demonstrate a correlation between the degree of cellular anaplasia and the occurrence of cell populations with increased DNA content in the tumours. Cells with diploid DNA content constituted more than 90% of well-differentiated tumours and normal bladder mucosa. Cell populations with aneuploid DNA content occurred in almost all biopsies with cellular anaplasia estimated as grades II and III by microscopic examination. The results indicate that FCM analysis may be a valuable adjunct to histology. The possible prognostic significance of altered DNA distribution in bladder tumours is discussed.

Cytological Techniques↗