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A J Kenyon

Publications and source records attributed to A J Kenyon.

13 recordsLinked to original sources
PubMed1978

Protides of the Mustelidae: immunoresponse of mustelids to Aleutian mink disease virus.

Members of North American Mustelidae were tested for their response to inoculation with 10(6) infective doses of Aleutian disease virus. In subfamily Mustelinae, 3 species in the genus Mustela (M vision, M erminea, and M putorius) and 2 species in genus Martes (Ma pennanti and Ma americana) responded immunologically with some features resembling Aleutian disease in mink. In subfamily Mephitinae, only Mephitis mephitis responded, and others of the subfamily did not, nor did members of subfamilies Melinae and Lutrinae. The responses observed ranged from development of detectable antibody levels determined by counterimmunoelectrophoresis to histopathologic changes typical of Aleutian disease.

Aleutian Mink Disease↗
PubMed1977

Properties of Aleutian disease virus assayed with feline kidney cells.

Properties of Aleutian disease virus (ADV) were studied using feline kidney cells, line CRFK, to assay virus by the induction of nuclear antigen. ADV nuclear antigen was detected by immunofluorescent staining. Titers of virus obtained from mink spleens at 10-8 days after infection were usually between 10(3) and 10(5) infectious units per gram of spleen. ADV was purified by fluorocarbon extraction, differential centrifugation, biogel A-15 chromatography and CsCl equilibrium centrifugation. The molecular weight of the virus was estimated to be 3-5 X 10(5) daltons. The density of antigen-inducing virus in equilibrium CsCl gradients was 1.32--1.34 g/cm3. On velocity sucrose gradients, antigen-inducing virus had a sedimentation coefficient of approximately 110S. The virus was not neutralized by sera from chronically infected mink and ferrets and by sera from experimentally infected mink. ADV was resistant to ionic and nonionic detergents and lipid solvents. The titer of partially purified virus was reduced as much as 700-fold by proteolytic enzymes but not by DNase or RNase. The virus was inactivated slowly at 56 degrees C; the initial half-life was 90 minutes. It is concluded that the properties of ADV can be determined by assay in CRFK cells, thus facilitating virological study of the disease.

Aleutian Mink Disease Virus↗
PubMed1977

Lymphoproliferative diseases of fowl: JM-V leukemic lymphoblasts in cell culture.

JM-V leukemic lymphoblasts were established in cell culture. The cultured cells (JM-VLC cells) were transplantable in young chicks and produced a disease indistinguishable from JM-V lymphoblastic leukemia as initiated by whole-blood inoculation. JM-VLC cells maintained a normal female karyotype through 13 passages in Rhode Island Red cockerels. With the use of JM-V antisera and antisera from birds with naturally occurring Marek's disease (MD), specific antigens were detected on the surfaces of living cells. Intracellular antigens were detected with anti-MD virus sera after cultivation for at least 1 day at 37 degrees C. In spite of the expression of MD antigens, the presence of herpesvirus particles associated with the cultured cells, and the occurrence of foci of multinucleated cells in kidney cultures from chicks inoculated with cellfree preparations of JM-VLC cells, the pathologic potential of the cultured cells was that of JM-V leukemia.

Animals↗
PubMed1977

Expression of Aleutian mink disease antigen in cell culture.

Infection of CRFK feline kidney cells with Aleutian disease vurus leads to production of virus-induced antigen(s) in the nucleus which could be demonstrated by the fluorescent-antibody technique. The number of fluorescent nuclei was lineraly dependent on the dilution of the inoculum, but rarely exceeded 20% of the cells. Aleutian disease nuclear antigen was only transiently detectable. The virus-induced antigen was detected after infection of cells of several divergent species; however, the CRFK line of feline kidney cells was the most susceptible. Inhibitor studies indicated that deoxyribonucleic acid synthesis, ribonucleic acid synthesis, and protein synthesis were required for viral antigen production. Cell growth was also a requirement for synthesis of viral antigen, An in situ radioimmune assay was used to measure binding of 125I-labeled mink anti-Aleutian disease virus to infected cells and competition with unlabeled sera. The system is suitable for quantitation of infectivity.

Aleutian Mink Disease↗
PubMed1976

Mobility of sodium dodecyl sulphate - protein complexes.

Reduced and unreduced lysozyme aggregates formed by formaldehyde cross-linking comprise a set of model compounds for studying the effects of protein conformation on the electrophoretic mobilities of sodium dodecyl sulphate-protein complexes. The reduced aggregates were indistinguisable from normal proteins, but the unreduced aggregates migrated anomalously fast by about 14%. Contrary to expectations, plots of logarithm Rf versus Kr (retardation coefficient) failed to reveal an unusual conformation for the unreduced aggregates. Thus the anomalous mobility caused by several intramolecular disulphide bonds escaped detection by the above two diagnostic plots. Also included in this paper is a discussion of the implications of these results with regard to current models for sodium dodecyl sulphate-protein complexes.

Electrophoresis, Polyacrylamide Gel↗
PubMed1976

Lymphoproliferative diseases of fowl: chromosome breaks caused in lymphocytes by JM-V herpesvirus.

Chromosome preparations were made of bone marrow cells and peripheral lymphocytes isolated from chicks that developed leukemia following infection with JM-V herpes-virus. Karyotypic analysis revealed a high frequency of chromosome breaks and aneuploidy, as well as some pulverization of chromosomes. The number of chromosome breaks began to increase at 2-3 days post infection, and by 5 days post infection it reached 12.7% of bone marrow cells and 17.2% of peripheral lymphocytes. Similarly, the number of aneuploid metaphase figures increased rapidly and reached 12% of bone marrow cells and 19% of peripheral lymphocytes at 5 days post infection. Some specificity was observed in the chromosomes that were affected.

Aneuploidy↗
PubMedDate not supplied

Lymphoproliferative diseases of fowl: characterization of transplantable G-B1 Marek's disease tumor cells in culture.

A transplantable Marek's disease tumor, derived and maintained through in vivo passage in syngeneic G-B1 chickens, was established in cell culture and characterized. The cells (GBT cells) grew in suspension and appeared morphologically similar to other Marek's lymphoblastoid lines except for prominent nucleoli in GBT cells. The number of chromosomes increased during in vitro cultivation from near triploid to subtetraploid. Chromosome no. 3 was abnormally short. The GBT cells retained surface B1 histocompatibility antigen of the original tumor as well as Marek's disease tumor-specific surface antigen (MATSA); however, infectious Marek's disease virus was not detected by immunofluorescence, electron microscopy, inoculation into susceptible birds, or assay on susceptible cells in culture. Inoculation of as few as 100 cultured cells into syngeneic G-B1 chickens results in tumor formation at the inoculation site, with eventual death of the recipients.

Animals↗