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Biomedical subjects

A J Hackett

Publications and source records attributed to A J Hackett.

At least 91 records · Page 5Linked to original sources
PubMed1971

Effect of rifampicin and two of its derivatives on cells infected with Moloney sarcoma virus.

It is shown that rifampicin, and especially the related antibiotic 2',6'-dimethyl-N(4')-benzyl-N(4')- [desmethyl]rifampicin (DMB-rifampicin) can inhibit focus formation by Moloney sarcoma virus on BALB/3T3 tissue cultures. At 10 mug/ml DMB-rifampicin totally inhibits focus formation while reducing virus replication by at least a factor of fifty and cell proliferation by only a factor of three. These observations, taken together with those of others, suggest a role for an RNA-dependent DNA polymerase and the gene for its synthesis both in normal cell processes and in the transformation process.

Animals↗
PubMed1968

Electron microscopic study of the morphogenesis of vesicular stomatitis virus.

Except for the rate, vesicular stomatitis virus (VSV) grows as well at 25 C as at 37 C in primary chick embryo fibroblast cells and in a pig kidney cell line [PK(H13)]. Maximal yields were reached at about 28 hr at 25 C and 10 hr at 37 C in these cells. Morphogenesis, as observed by electron microscopy, was similar at the two temperatures. The main feature was accumulation of virus in intracytoplasmic vacuoles. Mode of release of VSV has been controversial; both budding (as displayed by myxoviruses) and maturation at membranes of cytoplasmic vacuoles (as with arboviruses) have been claimed. Our observations support the latter view, and the apparent dichotomy in interpretation is discussed.

Animals↗