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Biomedical subjects

A J Berry

Publications and source records attributed to A J Berry.

65 records · Page 4Linked to original sources
PubMed1980

Blood levels of superoxide dismutase and glutathione peroxidase in Duchenne muscular dystrophy.

Erythrocyte superoxide dismutase activities and erythrocyte and plasma glutathione peroxidase activities have been determined in Duchenne muscular dystrophy patients, genetic carriers, and normal controls. A 19% decrease in red cell superoxide dismutase activity was observed in patients with Duchenne muscular dystrophy. Genetic carriers showed a level between that of the dystrophy patients and the normal controls. No abnormality was seen in the red cell or plasma activities of glutathione peroxidase.

Adolescent↗
PubMed1977

Sensitive, optimized assay for serum AMP deaminase.

A sensitive, optimized assay for serum AMP deaminase (EC 3.5.4.6) is presented, which is based on a colorimetric indophenol determination of ammonia liberated during the enzyme reaction. An average enzyme activity in serum of 2.7 U/liter (n = 36, range 0-6.2) for healthy adults was established. Both the enzyme and colorimetric reactions were optimized to give an assay sensitivity of 0.2 U/liter of serum, a 98-99% analytical recovery in the normal serum enzyme concentration range, and a CV of 7.9% in-run and 10.6% between-run. This optimization included studies on specific buffer, pH, ionic strength, and potential activators. The Km for the enzyme in serum was determinated to be 1.4 X 10(-3) mol/liter, which agrees well with the value reported for human skeletal muscle enzyme. Above-normal serum AMP deaminase activity may be present in various myopathies and could be useful in detecting carriers of the X-linked dystrophies.

AMP Deaminase↗