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Biomedical subjects

A Iu Krol'

Publications and source records attributed to A Iu Krol'.

7 recordsLinked to original sources
PubMed1996

[The attachment of erythroid cells to solid supports in the presence of lectins].

A comparative influence of lectins on the attachment kinetics of erythroid cells (human erythrocytes and erythroleukemia cells K562) to glass formvar has been studied. It is shown that the inhibiting effect of wheat germ agglutinin (WGA) on the adhesion to glass is the same for both types of cells under study, whereas concanavalin A (Con-A) inhibits the erythrocyte adhesion to glass only but it is ineffective for adhesion of K562 cells. Plasmatic membranes of K562 cells are free from band 3 protein contrary to the erythrocyte ones. It is concluded that the inhibition of erythrocyte adhesion by lectins results from their binding to different components of cell membranes; sialic acid residues of glycophorin (WGA) or band 3 protein (Con-A). This conclusion is confirmed by the inhibiting effect of WGA towards attachment of cells K562 to glass and the absence of such an effect of Con-A towards the same cells which do not contain this protein. The most probable form of both cell type attachment to glass is the occurrence of so-called focal contacts. The latter can be easily observed in the case of K562 cells. The intact erythrocyte attachment to formvar occurs slower than to glass. At the same time, the above lectins accelerate the erythrocyte attachment to formvar.

Cell Adhesion↗
PubMed1989

[The suppression of rapid local surface oscillations in human erythrocytes slows and weakens their adhesion to glass].

Rapid local oscillations of the erythrocyte surface with amplitude 200-300 nm are decreased by 10 times following addition of wheat germ agglutinin (10(-77) M). In this case the rate of erythrocyte adhesion to the cover glass is delayed approximately by 3-9 times. The total suppression of erythrocyte surface oscillations occurs in hypo-osmic solution or in a 0.01% solution of glutaraldehyde. It coincides with a two-fold decrease of erythrocyte adhesivity to the glass. It is suggested that the rapid erythrocyte surface oscillations may control the rate of cell adhesion to the substrate.

Acetylglucosamine↗
PubMed1989

["Spontaneous" oscillations of the cell surface in the frequency interval of 0.2-30 Hz].

The local transverse displacements of the cell surface within the frequency range of 0.2-30 Hz occur on animal cells attached to cover glass: fibroblasts 3T6, primary culture of rat cardiomyocytes, mouse lymphocytes, human macrophages and erythrocytes, frog erythrocytes. The area of the cell rim, moving transversely, is no more than 0.5 X 0.5 microns. The maximum amplitude of the local transverse surface displacement is different in various cell types, being maximum (350-400 nm) in human erythrocytes and minimum in fibroblasts 3T6, mouse lymphocytes and human macrophages (20-30 nm). High-amplitude oscillations of human erythrocytes and low-amplitude oscillations of frog erythrocytes correlate with differences in the size of elastic shear modulus.

Animals↗
PubMed1989

[Rapid local oscillations of the surface of the human erythrocyte].

The transverse displacements of the human erythrocyte surface with amplitude 300-400 nm in the frequency range 0.2-30 Hz are recorded on the minimal area erythrocyte rim (approximately 0.5 X 0.5 microns). These local oscillations of the surface are diminished at hypoosmotic erythrocyte swelling, on addition of substances which increase the membrane rigidity (0.01% glutaraldehyde, 0.5 mM 4-hydroxymercuribenzoate, cell membrane stain--0.002% Heliogen Blue) and on discocyte--echinocyte transformation due to addition of 1-2 mM 2,4-dinitrophenol. The amplitude of transverse displacements is reduced by 1.7-2 times on erythrocytes of patients with inherent microspherocytosis. These erythrocytes have inherent defects in spectrin. It is suggested that spectrin is important for rapid local oscillations of the human erythrocyte surface.

Elasticity↗