Biomedical subjects

A Ittenson

Publications and source records attributed to A Ittenson.

21 records · Page 2Linked to original sources

PubMed1985

[Human T-lymphocytes--clinical and methodological studies on the detection of Fc-IgM- and Fc-IgG-receptor bearing T-lymphocytes (Tm and Tg)].

Several methodical aspects for determination of T lymphocytes with Fc receptors for IgM (TM) and IgG (TG) were studied including separation technique of T cells with E-rosetting, culture conditions of T cells for determination of TM and the rosetting of TM and TG with EA complexes. The bests results were obtained by stabilization of E-rosettes with human serum albumin, after separation of E-rosette forming cells lysis of sheep erythrocytes with save hypotonic shock, culturing of T cells in medium containing 20% AB Serum. Furthermore it was shown the possibility using EA complexes produced with not purifieded IgG or IgM anti-ox-red-blood cells antisera without lost of specifity for TM and TG. The percentage of TM and TG in peripheral blood of thirty healthy persons as well as monitoring TM and TG in three cases was investigated.

Humans↗

PubMed1984

Dipeptidyl peptidase IV and alpha-naphthylacetate esterase in human T lymphocytes: cytochemical and biochemical investigations.

Dipeptidyl peptidase IV (DP IV) activity of human lymphocytes was measured using biochemical assays of cell suspensions and enzyme cytochemical staining of smears from capillary blood and mononuclear cells (MNC). The hydrolysis rate of Gly-Pro-pNA in suspensions of MNC correlates well with the number of DP IV reactive cells as determined by cytochemical staining. MNC from healthy volunteers were shown to contain 44 +/- 10% lymphocytes reactive for DP IV. Using preparations of T lymphocytes and adherent MNC it was shown that DP IV is specific for T lymphocytes. About 60% of T lymphocytes contain DP IV and 94% of DP IV reactive cells form rosettes with sheep erythrocytes. Parallel staining for DP IV and unspecific acid alpha-naphthylacetate esterase (ANAE) yielded nearly the same figure of cells stained for ANAE in a dot-like pattern (50 +/- 10% MNC) as was observed for DP IV. Correlation of both markers indicates that DP IV expressing lymphocytes presumably represent that subpopulation of TM cells which is characterized by a dot-like reaction pattern of ANAE.

Dipeptidyl Peptidase 4↗

PubMed1984

[Lymphocyte separation with dextran M 70].

We investigated the possibility using Dextran M 70 instead of the common Ficoll 400, both in combination with Visotrast 370, for separation of lymphocytes from human peripheral blood by density gradient centrifugation. No significant differences were found with regard to parameters such as yield and vitality and numbers of B- (surface immunoglobulins) and T-lymphocytes (sheep red blood cells-rosette assay, and the monoclonal antibody BL-T2). From the data available as yet we can conclude--at least for the markers studied--that it is possible to use Dextran M 70 instead of Ficoll 400 for the separation of mononuclear cells of human peripheral blood.

Cell Separation↗