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Biomedical subjects

A Irie

Publications and source records attributed to A Irie.

106 records · Page 6Linked to original sources
PubMed1986

Human urinary prokallikrein: rapid purification and model activation by trypsin.

With only a three-step chromatographic procedure, human urinary prokallikrein has been purified completely. The active kallikrein also could be purified in the process of this purification. The prokallikrein was very rapidly activated by trypsin, followed thereafter by a very slow increase in the kallikrein activity. In the rapidly activated state, the molecular weight and the values of Km and Vmax were very similar to those of the purified active kallikrein. Only the slow increase in the activity was observed by tryptic digestion of the active kallikrein. The results suggest that the initial rapid activation is due to release of the propeptide and the slow reaction is due to a limited hydrolysis of the activated prokallikrein at sites which are not directly related to the active site.

Chromatography, Affinity↗
PubMed1980

Absence of transcellular fluid shift during haemofiltration.

Total body and extracellular fluid volume before and after haemofiltration were calculated using urea and inulin as markers. The change in plasma volume and interstitial fluid volume were also evaluated by haemoglobin concentration and hematocrit value. The extracellular fluid volume, especially interstitial fluid volume, was reduced, while intracellular fluid volume was not altered by haemofiltration.

Blood↗
PubMed1979

Synthesis of 2,3,4,4a,5,9b-hexahydro-1H-pyrido[4,3-b]indole derivatives and their central nervous system activities.

The synthesis and some pharmacological effects of cis- and trans-2-substituted 2,3,4,4a,5,9b-hexahydro-1H-pyrido[4,3-b]indole derivatives are described. In these derivatives, the substituents of the 2, 5 and 8 position, together with the relative configuration of the 4a and 9b position, influenced the potency of the central nervous system activities. A cis-2-[3-(p-fluorobenzoyl)propyl] analogue (5k) of carbidine (1) possessed not only thymoleptic-like biological activity but had more potent neuroleptic activity than the parent drug.

Animals↗
PubMedDate not supplied

Surgical risks of acute cholecystitis in elderly.

BACKGROUND/AIMS: For the elderly patient, an emergency biliary procedure carries a higher risk than an elective operation. Recently introduced advances in ultrasonography and critical care medicine have affected the clinical risks of surgery for acute cholecystitis in the elderly. This study evaluated the clinical risks of open cholecystectomy for the elderly with acute cholecystitis. MATERIALS AND METHODS: During a 10 year period (1985-1994), a total of 145 patients were diagnosed with acute cholecystitis and underwent cholecystectomy. According to their age, the patients were divided into 3 groups (Group A < 59 years of age; Group B between 60-69 years of age; Group C > 70 years of age). The characteristics and the surgical risk factors in open cholecystectomy for the elderly with acute cholecystitis were evaluated. RESULTS: The rate of acalculous cholecystitis and choledochal stones were significantly (p < 0.05) high in Group C. Septic complication, gangrenous changes, and positive bile culture were also increased parallel to the increase in age. A noteworthy finding was an incidental carcinoma found in a case in group B and in 3 cases in group C. Hospital stay was significantly longer in Group C than in the other groups due to pre-operative complications and post-operative morbidity. CONCLUSION: With respect to increase in elderly patients with acute cholecystitis who present more frequent gangrenous changes and carcinomatous changes as well as high rate of septic complication, successful treatment of these patients is increased when early surgery can be performed on the basis of accurate and prompt diagnosis using imaging modalities and meticulous peri-operative management.

Acute Disease↗