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Biomedical subjects

A Iida

Publications and source records attributed to A Iida.

At least 145 records · Page 8Linked to original sources
PubMed1979

Investigation of an intracellular hemolytic agent of Vibrio parahaemolyticus. Lipid fraction of dried cells.

The dried cells of two strains of Vibrio parahaemolyticus were fractionated by extracting first with water and then with organic solvents. The hemolytic activity of the fractions was determined, and some of them were assayed for their effect in mice. The hemolytic agent present in the water-insoluble fraction was extractable in organic solvents such as 70% aqueous ethanol, chloroform-methanol-water (1:2:0.8) and acetone. The extracts showed no toxic effect in mice after intraperitoneal inoculation. No hemolytic activity was observed in the remaining cell residue, which bore the toxicity.

Animals↗
PubMedDate not supplied

Improvement of multiple pathophysiological phenotypes of klotho (kl/kl) mice by adenovirus-mediated expression of the klotho gene.

BACKGROUND: We have established a novel mouse mutant, klotho (kl), by insertional mutation of a transgene and identified the structural gene. The mouse homozygous for the mutation exhibits multiple pathological conditions resembling age-related disorders in humans and can be regarded as a model of human premature aging syndromes. However, the pathophysiological role of Klotho protein has not been clarified. METHODS: A replication-deficient adenoviral vector expressing the membrane form of the mouse klotho gene was constructed and we examined Klotho expression in vitro. The recombinant adenoviral vector was then administered intravenously into klotho mice at 4-5 weeks of age and its therapeutic potential was examined. RESULTS: Expression of Klotho protein was observed in the adenoviral vector-infected CHO cells. The klotho mice infused with the recombinant adenovirus showed a significant extension of life span and gain in body weight at 1 week after treatment. Macroscopic and histological analyses demonstrated the improvement of multiple pathological findings such as restoration from atrophy and cell formation and differentiation in the gonadal cells, immune tissues and subcutaneous fat. CONCLUSION: We showed that local expression of the klotho gene retards or partially improves pathological abnormalities in several organs of klotho mice after onset of the phenotypes. Therefore, the recombinant adenovirus vector will provide an important tool for investigating the molecular mechanism of the Klotho protein and give clues to understanding the individual disease mechanisms.

Adenoviridae↗
PubMedDate not supplied

Prognostic value of DNA ploidy and proliferating cell nuclear antigen in gastric cancer.

DNA ploidy and the labeling index (LI) of proliferating cell nuclear antigen (PCNA) in gastric cancers were determined using cytofluorometry and immunohistochemistry, respectively, and the prognostic value of these two parameters was evaluated. Of 66 patients with advanced gastric cancers treated with radical resection, 27 with aneuploidy and a high PCNA-LI (> or = 40) showed the lowest 5-year survival rate (38%). Twenty patients with diploid cancers and a high LI showed a lower 5-year survival rate (59%) than the 15 patients with diploid cancers and a low LI (<40), who had the highest 5-year survival rate (86%). A multivariate analysis showed that the grouping based on the ploidy and the LI was an independent prognostic factor. Thus, the combination of DNA ploidy and PCNA-LI may be a useful prognostic indicator for advanced gastric cancers.

Adult↗
PubMedDate not supplied

Relationships among the expression of epidermal growth factor receptor, proliferating cell nuclear antigen labeling index, and lymph node metastasis in gastric cancer.

The expression of epidermal growth factor receptor (EGFR), epidermal growth factor (EGF), transforming growth factor alpha (TGF alpha) and the labeling index of proliferating cell nuclear antigen (PCNA LI) were examined immunohistochemically in 288 gastric cancer patients, and the relationships between these results and the lymph node metastasis were studied. To investigate the relation between the expression of EGFR and PCNA LI, we divided the patients into the following three groups according to the immunohistochemical findings: group A, EGFR (+); group B, EGFR (-), and EGF(+) or TGF alpha(+); group C, EGFR(-), EGF(-) and TGF alpha(-). In the cancers invading submucosal or proper muscle layer, high-PCNA tumors (PCNA LI > or = 70) in both groups A and B had more frequent lymph node metastasis than in the intermediate-(40-69) and low- (< or = 39) PCNA tumors. In the cancers invading subserosal layer or further, the frequency of metastasis in group A was over 78% and was not related to the PCNA range. In group B, metastasis was more frequent in high- and intermediate-PCNA tumors (about 80%) than in low-PCNA tumors (44%). These results suggest that growth regulation by EGFR is related to lymph node metastasis in gastric cancer, and the higher the PCNA LI of cancer cells becomes, the more frequent the occurrence of lymph node metastasis.

Adult↗
PubMedDate not supplied

Surgical results of pancreaticoduodenectomy for carcinoma of the distal third of the stomach.

Pancreaticoduodenectomy (PD) was performed as a radical operation on 10 patients who had stage III or IV carcinoma of the distal third of the stomach which invaded the pancreas head (T4) or had level 3 lymph node metastasis. The surgical results of the PD were compared with those of 69 patients treated with subtotal gastrectomy (SG). Although the postoperative morbidity was higher (70%) in the PD group than in the SG group (32%), no hospital death occurred. The overall postoperative survival provided by PD was as good as that provided by SG for 43 patients who had stage III or IV tumors (the 5-year survival rates, 40 versus 45%). Regarding the T4 tumors invading the pancreas, the survival of the 9 patients with PD was better than that of the 12 patients with SG (median survival time, 19 versus 9 months). Thus, PD might improve the postoperative survival of patients with carcinoma of the distal stomach invading the pancreas.

Adult↗