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Biomedical subjects

A Ihara

Publications and source records attributed to A Ihara.

28 records · Page 2Linked to original sources
PubMed1992

Specific acceptance of cardiac allograft after treatment with antibodies to ICAM-1 and LFA-1.

An indefinite survival of cardiac allografts between fully incompatible mice strains was observed when monoclonal antibodies (MAbs) to intercellular adhesion molecule-1 (ICAM-1) and leukocyte function-associated antigen-1 (LFA-1) were simultaneously administered after the transplantation for 6 days. Mice with long-term surviving cardiac allografts accepted skin grafts from the donor-strain but rejected skin grafts from a third-party strain. Because MAbs to ICAM-1 or LFA-1 alone were insufficient for prolonged tolerance, the two MAbs probably acted synergistically to induce specific unresponsiveness. Thus, ICAM-1----LFA-1 adhesion participates in the induction of allograft rejection and MAbs may be useful as therapeutic agents.

Animals↗
PubMed1992

[Establishment and characterization of a new poorly differentiated gastric cancer cell line (MKK-1), derived from Borrmann type 3 tumor].

A new human gastric cancer cell line (MKK-1), derived from Borrmann type 3 tumor of the stomach, was established. It was obtained from poorly differentiated adenocarcinoma. The cell line grew in vitro, forming a sheet of monolayered cells and attaching firmly to the inner surface of culture vessels; it continued to grow for more than 1 year and 4 months. Its doubling time was 12.6 hours, with a chromosomal mode of 69. The cells could grow in nude mice; histological findings of the tumors developed in the mice showed a pattern similar to those in the primary tumor, i.e., poorly differentiated adenocarcinoma. With regards to tumor-related antigens, production of the CA19-9 was observed as time-dependent increase and was detected at a level of 4 U/ml in the culture supernatant on passage day 7, showing high values compared with the control. Immunostaining was positive for both the anti CEA antibody and the anti CA19-9 antibody. In sera examined in week 4 after subcutaneous transplantation of MKK-1 to the nude mice, CEA and CA19-9 levels were high, at 8.93 ng/ml and 44 U/ml, respectively. There was a positive correlation between increase in a tumor weight and the production of tumor-related antigens.

Adenocarcinoma↗
PubMedDate not supplied

Three-dimensional reconstruction of colorectal tumors from serial tissue sections by computer graphics: a preliminary study.

BACKGROUND/AIMS: We present herein the three-dimensional reconstruction of colorectal tumors, with particular reference to growth pattern into each layer of the colorectal wall, and measurement of tumor volume and surface area. METHODOLOGY: Conventional tissue section images of colorectal tumors were analyzed using a computer graphics analysis program. The two-dimensional extent of invasion by each tumor into each layer of intestinal wall were determined from the images of each section. Based on data from multiple sections, tumor and surrounding normal tissue layers were reconstructed three-dimensionally, and volume and surface area of the tumors were determined. RESULTS: Using this technique, three-dimensional morphology of tumor and tumor progression into colorectal wall could be determined. Volume and surface area of the colon tumor were 4871 mm3 and 1741 mm2, respectively. Volume and surface area of the rectal tumor were 1090 mm3 and 877 mm2, respectively. CONCLUSIONS: This technique may provide a new approach for pathological analysis of colorectal carcinoma.

Colonic Neoplasms↗