Role of the cro gene in bacteriophage lambda development.
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Binding Sites↗
Biomedical subjects
A Honigman
Publications and source records attributed to A Honigman.
In vitro effects of some chemotherapeutic agents on mycobacteria.
Examination of the bactericidal and bacteriostatic effects of nine antibiotics on atypical mycobacteria revealed that streptovaricin complex and streptovaricin C exerted bactericidal effects on several strains in concentrations lower than 1.0 mug/ml. An exposure to the drug for 48 hr at 37 C was necessary to effect a complete inactivation of more than 99.9% of the exposed microorganisms. The appearance of strepto-varicin-resistant mutants was observed. However, these mutants were unstable, and reversion to streptovaricin susceptibility occurred. Celesticetin salicylate, added in a concentration of 100.0 mug/ml to the medium of Olitzki and Gershon inoculated with Mycobacterium leprae, effected a complete change of the uniformly stained mycobacteria to bipolarity, which indicates the devitalization of this microorganism.
Anti-Bacterial Agents↗
Studies on atypical mycobacteria in Israel. I. Infections caused by Mycobacterium fortuitum.
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Adult↗
Herpes simplex virus type 1 thymidine kinase gene expression in Escherichia coli.
The HSV-1 thymidine kinase (TK) gene of Herpes simplex virus was inserted into plasmids pBR322, pMOB45 and pHA10. The recombinant hybrid plasmids were used to transfect a tdk- mutant of Escherichia coli (Ky895) and the synthesis of the viral TK in the bacterial host was studied. Recombinant plasmids containing the entire BamHI-BamHI DNA fragment carrying the viral TK gene and the upstream sequences containing its promoter were able to produce the thymidine kinase, but removal of the BamHI-BglII DNA fragment containing the viral promoter of the TK gene resulted in enhanced expression of the viral gene, originating from the pBR322 tetr gene promoter. When the BglII-BamHI DNA fragment containing the viral TK gene was cloned in a plasmid with temperature-dependent copy number control (pMOB45), expression of the TK gene was enhanced fourfold by the temperature shift. Cloning of the HSV-1 TK gene in plasmid pHA10 showed that the lambda pL promoter allowed transcription of the viral gene but not the synthesis of active TK. The BamHI-BglII DNA fragment containing the viral upstream sequences with the promoter of the TK gene was found to contain transcription-termination sequences that prevented expression of the lambda kil gene of pHA10 in the presence of lambda N function.
DNA, Recombinant↗