Fingertip lesions. An evaluation of conservative treatment versus free skin grafting.
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Amputation, Surgical↗
Biomedical subjects
A Holm
Publications and source records attributed to A Holm.
Synthesis and screening of an indexed motif-library containing non-proteinogenic amino acids.
In an effort to increase the probability of finding novel peptides in resin-bound combinatorial libraries displaying affinity to various macromolecular targets, we increased the diversity of a solid-phase library considerably by synthesizing multiple structures on each bead - a motif-library - including 45 building blocks. The building blocks consist of L-aa, D-aa and eight hydrophobic non-proteinogenic alpha-amino acids. A library with the format O-Z0-1-O-Z0-1-O-XX-resin was synthesized giving the four motifs OOOXX, OZOOXX, OOZOXX, OZOZOXX corresponding to 364.500 different motifs (45(3) x 4 theoretical combinations). The positions O are defined amino acids while Z represents three mixtures pi, omega, phi, where pi is a mixture of polar and charged residues, omega is a mixture of aliphatic residues and phi is a mixture of aromatic residues. X represents a mixture of all 45 residues. The library was screened with the macromolecular target streptavidin which served as a model receptor. Binding peptides were sequenced by microsequencing. We included small amounts of norvaline and norleucine in the library, which served as index residues to be able to distinguish between LD-amino acids and other residues with the same retention time in the HPLC system. Beads that interact with the receptor were found, and the binding motifs that appeared had no homology to known binding motifs found in either L-aa or D-aa libraries, instead motifs with the non-proteinogenic residues L-phenylglycine, O-benzyl-L-hydroxyproline and O-benzyl-L-tyrosine dominated. The novel peptides inhibit binding of biotin to streptavidin but do not bind to avidin, and the affinity is higher than the peptides found in linear all L-aa peptide libraries.
Amino Acids↗
Mapping and identification of HeLa cell proteins separated by immobilized pH-gradient two-dimensional gel electrophoresis and construction of a two-dimensional polyacrylamide gel electrophoresis database.
The HeLa cell line, a human adenocarcinoma, is used in many research fields, since it can be infected with a wide range of viruses and intracellular bacteria. Therefore, the mapping of HeLa cell proteins is useful for the investigation of parasite host cell interactions. Because of the recent improvements of two-dimensional gel electrophoresis with immobilized pH gradients (IPG) compared to isoelectric focusing with carrier ampholytes, a highly reproducible method for examining global changes in HeLa cell protein expression due to different stimuli is now available. Therefore, we have initiated the mapping of [35S]methionine/cysteine-labeled HeLa cell proteins with the 2-D PAGE (IPG)-system, using matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) and N-terminal sequencing for protein identification. To date 21 proteins have been identified and mapped. In order to make these and future data accessible for interlaboratory comparison, we constructed a 2-D PAGE database on the World Wide Web.
Acrylic Resins↗
Characterization of Chlamydia trachomatis l2-induced tyrosine-phosphorylated HeLa cell proteins by two-dimensional gel electrophoresis.
Chlamydia trachomatis is an obligate intracellular bacteria, inducing its own uptake in nonprofessional phagocytes either by phagocytosis or pinocytosis. We have previously shown that C. trachomatis L2 induces tyrosine phosphorylation of eukaryotic proteins upon their entry by phagocytosis. In this paper we characterize the tyrosine-phosphorylated proteins by two-dimensional gel electrophoresis. In immunoblotting with anti-phosphotyrosine antibodies of C. trachomatis L2-infected HeLa cells, but not with uninfected cells, two rows of spots were observed with a molecular mass of 69 and 71 kDa and pI from 5.0 to 5.2. In addition, a single spot of 100 kDa and pI 6.2 was observed.
Animals↗
Immunosuppression (Neoral vs Sandimmune) in pediatric kidney transplantation.
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Administration, Oral↗
New Cadaveric Organ Donation Program at the Social Security Mexican Institute: initial experience.
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Brain Death↗
A new method for the synthesis of neoglycopeptides.
A new method for conjugation of small carbohydrates to peptides based on maleimido-thiol chemistry is described. The reducing carbohydrate is reacted with S-trityl-2-mercaptoethylamine in methanol. The resulting Schiff base is then stabilized by acetylation with acetic anhydride to give an S-trityl-protected glycosylamide. Activation of the carbohydrate is effected by brief treatment with trifluoroacetic acid/triisopropylsilane, followed by precipitation in ether. The thiol-functionalized carbohydrate is then reacted with a maleimido-modified peptide to give a neoglycopeptide. The potential of this method was investigated using maltose as the model compound. Furthermore, we prepared five maleimido-modified model peptides. In contrast to some literature reports, we found that the maleimido group is stable to treatment trifluoroacetic acid/triisopropylsilane which was used to cleave the modified peptides from the resin.
Acetylation↗
Phonological awareness skills of 4-year-old British children: an assessment and developmental data.
This study reports developmental data for the phonological awareness and processing skills of 57 normally developing Tyneside preschool children, aged between 46 and 58 months. The children were assessed on eight tasks: consistency of word production, phonological variability according to speech production task, non-word imitation, syllable segmentation, rhyme awareness, alliteration awareness, phoneme isolation and phoneme segmentation. The results indicated that girls and boys performed similarly; socio-economic status significantly affected performance on six of the tasks; and age was significantly correlated with performance on tasks targeting alliteration, non-word imitation, phonological variability, and phoneme isolation and segmentation. The older children were more phonologically aware than the younger children.
Awareness↗
Comparison of cross-language generalisation following speech therapy.
Little is known about the phonological development of children who acquire two languages sequentially in the preschool years. Some of these children will be referred for assessment of speech disorder. Distinguishing between delayed development due to the language learning environment and disorder is problematic in the absence of normative data on the typical phonological development of bilingual children. Another major issue concerns whether both languages require intervention, or only one because of generalisation to the other language. Treatment efficacy studies of 2 bilingual children are reported. The data indicate that different patterns of cross-language generalisation occur depending upon the deficit in the speech processing chain underlying the speech disorder.
Articulation Disorders↗
Arterial pressure measurements correlated to symptoms and signs of peripheral arterial disease.
The systolic pressure observed in 150 patients with peripheral arterial disease has been compared to their symptoms and signs. In patients with claudication the ankle mean pressure was 58 mmHg. In patients with rest pain it was 33 mmHg and in patients with chronic ulcerations it was 20 mmHg. In these 3 groups the mean ankle-foot pressure gradient was low (2-10 mmHg) were detected at the level of the iliaco-femoral and femora-popliteal segments. In the group of diabetic patients an high gradient was observed. Patients with peripheral arterial disease can be divided in four symptomatic groups but the angiographic and physiological patterns of patients with rest pain and ischemic ulcerations are similar and they are the best candidates to reconstructive arterial surgery.
Arterial Occlusive Diseases↗
Practical multipeptide synthesis: dedicated software for the definition of multiple, overlapping peptides covering polypeptide sequences.
A personal computer program for the conversion of linear amino acid sequences to multiple, small, overlapping peptide sequences has been developed. Peptide lengths and "jumps" (the distance between two consecutive overlapping peptides) are defined by the user. To facilitate the use of the program for parallel solid-phase chemical peptide syntheses for the synchronous production of multiple peptides, amino acids at each acylation step are laid out by the program in a convenient standard multi-well setup. Also, the total number of equivalents, as well as the derived amount in milligrams (depend-ending on user-defined equivalent weights and molar surplus), of each amino acid are given. The program facilitates the implementation of multipeptide synthesis, e.g., for the elucidation of polypeptide structure-function relationships, and greatly reduces the risk of introducing mistakes at the planning step. It is written in Pascal and runs on any DOS-based personal computer. No special graphic display is needed.
Amino Acid Sequence↗