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Biomedical subjects

A Hinek

Publications and source records attributed to A Hinek.

At least 91 records · Page 5Linked to original sources
PubMed1976

In vitro elastic fiber formation by aggregated arotic cells of newborn rabbits.

Cells isolated enzymatically from the aortas of newborn rabbits were aggregated and grown in organ culture. Bundles of microfibrils, some of them with an amouphous core (elastin), were evident in 3-day-old aggregates. Furthermore, droplets of elastin surrounded by darker dots and short filaments, corresponding to the elastic units were observed. In 6-day-old aggregates the number of elastic fibers composed of bundles of microfibrils with deposited elastin increased. Elastic elements which probably resulted from a coalescence of elastic units were also present. These two ways of elastic fiber formation in aggregates are compared with those described in papers on aortic development.

Animals↗
PubMed1976

Heterotopic allotransplantation of isolated aortic cells. An electron microscopic study.

Smooth muscle cells were enzymatically isolated from the tunica media of the aorta of 5-day-old rats. Following in vitro exposure to colloidal thorium dioxide particles the cells displayed numerous lysosomes filled with these particles. Intramuscular injection of isolated cells into the tongues of young rats of the same strain, resulted in the formation by both freshly isolated and thorium dioxide-labeled cells of a characteristic tissue similar to that found in the intact aortic wall. Thus, the transplants consisted of typical smooth muscle cells surrounded by an extracellular matrix consisting of a microfibrillar network, patches and fibers of elastin, collagen fibrils and small polygonal granules believed to represent proteoglycans. This system can be used for experimental studies on the production of extracellular matrix components and on other functions of arterial smooth muscle cells growing outside the vascular wall.

Animals↗
PubMed1975

Comparison of morphology of isolated cells obtained from aortas of normal and cholesterol fed rabbits.

Cells from aortas of healthy newborn and adult rabbits were liberated by digestion with trypsin and collagenase. In the same way were obtained free cells from the aortas of rabbits with far advanced experimental atherosclerosis. More than 90 p. 100 of the cells from all groups were viable. Isolated cells were used for electron microscopic examination. In material obtained from aortas of newborn rabbits, endothelial cells, fibroblasts and smooths muscle cells were present. In adult rabbits two kinds of endothelial cells, fibroblasts, several varieties of myocytes and foam cells were found. The bulk of aortic cells isolated from rabbits with experimental atherosclerosis consisted of endothelial cells and smooth myocytes. The cytoplasm of all myocytes contained lipid vacuoles. The lipid-loaded myocytes corresponded to the typical foam cells. Lipid content was relatively scanty in the endothelial cells. The presence of myocytes foam cells like in the aortas of healthy adult rabbits, identical as in material from experimental atherosclerosis, may support the view that these cellular changes so far considered as typical for atherosclerosis are common, and may be treated as an exponent of the natural process of aging of the vascular wall.

Animals↗
PubMedDate not supplied

Electron microscopic studies on cells isolated from aorta of newborn and adult rabbits.

Cells from aortas of newborn and adult rabbits were liberated by digestion with trypsin and collagenase. More than 90% of them were viable at the end isolation. Isolated cells were used for electron microscopic examination. In the material obtained from aortas of newborn rabbits endothelial cells, fibroblasts and smooth muscle cells were present. In material from adult rabbits two kinds of endothelial cells, fibroblasts, several varieties of myocytes, fat-laden macrophages and typical foam cells were found. Various forms of myocytes seem to represent different functional stages or specialized kinds of these cells. The presence of fat laden macrophages and foam cells in the aortas of healthy adult rabbits may support the view that these cellular changes so far considered as typical of atherosclerosis are common and may be treated as an exponent of natural process of aging of vascular wall.

Age Factors↗
PubMedDate not supplied

The influence of mestranol and chlormadinone given separately or in combination on the effects evoked by a high-fat diet in female rabbits.

The effect of mestranol and chlormadinone, the hormones contained in oral contraceptives: on lipid metabolism, some hemostatic parameters and on morphology of aorta, liver and heart was tested after separate or combined administration to mongrel female rabbits fed on a normal or high-fat diet (HFD). HFD administration led to typical atheromatous changes in the aortal intima. The hormones given together with HFD attenuated the augmentation of lipid serum level and the extent of atheromatous plaques in the intima. The percent content of alpha-lipoproteins was significantly lower in groups receiving HFD with or without the hormones than in the controls, but it was higher than in controls in the rabbits receiving the hormones alone. Chlormadinone given together with HFD potentiated atheromatous aortal changes, which occurred not only in the intima but also in the media. Mestranol given together with HFD significantly counteracted the development of atheromatous changes. Female rabbits receiving mestranol and chlormadinone displayed a significant increase in fibrinogen content, while those receiving mestranol showed an increased time of fibrinolysis. HFD produced a hepatic damage, which was augmented in rabbits concomitantly receiving the hormones. The hormones alone also displayed some, a less pronounced hepatotoxic action.

Animals↗
PubMedDate not supplied

Protective effect of inosine against adrenaline toxicity in rats.

The effect of inosine (200 mg/kg ip) injected 30 and 10 min before adrenaline (AD) administration to rats was studied. AD in the single dose (2 mg/kg sc) evoked heart necrosis evidenced by plasma activities of CPK-MB, CPK, blood lactates concentrations and histopathological examination. Inosine pretreatment caused less pronounced enhancement of enzyme activities and lactate concentrations in both experimental groups. Histopathological examination confirmed the protective effect of inosine on adrenaline-induced myocardial necrosis.

Animals↗
PubMedDate not supplied

The effect of amino acid derivative of clofibric acid (WKLB-5), and theophylline monoester of nicotinic acid (ME1) on the development of experimental atherosclerosis in rabbits.

In order to determine the efficacy of new compounds--an amino acid derivative of clofibric acid (WKLB-5), and a theophylline monoester of nicotinic acid (ME1)--a high fat diet (HFD) was applied to rabbits for 3 months, with or without the above mentioned compounds. The HFO included coconut oil, cholesterol and cholic acid. A substantial hypolipemic activity of those compounds was demonstrated; however, the studied lipid fractions did not undergo normalization, except FFA whose level after ME1 administration was distinctly lower than in control animals. Sudanophilic changes were found on 83% of surface of the interior membrane of aorta in rabbits kept on the HFD only, on 35% of surface--in rabbits treated with the HFD + WKLB-5, and on 75% of surface--in rabbits treated with the HFD + ME1. Thus in those animals no parallelism between the hypolipemic and antiatherosclerotic activities was observed.

Animals↗
PubMedDate not supplied

Adriamycin-induced cardiomyopathy. A rat model.

A rat model of adriamycin-induced cardiomyopathy was elaborated. Adriamycin (ADR) in three total doses 21, 25 and 30 mg/kg ip injected in 15 equal partial doses caused the loss in body weight and hematocrite values, marked leucopenia and moderate elevation of SGOT activities as well as ECG changes. In histopathological examination cardiomyopathic changes were found in heart samples from rats treated with two higher ADR doses. The dose 25 mg/kg is suggested for further investigations concerning the limitation of ADR toxicity.

Animals↗
PubMedDate not supplied

The effect of colchicine on the development of experimental atherosclerosis in rabbits.

The effect of colchicine on the course of experimental atherosclerosis was investigated in rabbits receiving for 3 months a high-lipid diet (HLD) in addition to the basal diet. Colchicine was given in a dose of 0.2 mg/kg ip twice a week. An evident antiatherosclerotic action of colchicine was demonstrated both by macroscopic and microscopic investigation of the aorta. The level of blood total lipids was significantly depressed, and the level of triglycerides was back to normal. The content of total lipids in liver homogenates was similar to that in the controls. A significant decline in the fibrinogen concentration in the blood of rabbits receiving colchicine was also found.

Animals↗
PubMedDate not supplied

A search for a model of experimental atherosclerosis: comparative studies in rabbits, guinea pigs and rats.

Rabbits, guinea pigs and rats received for 3 months a high lipid diet containing coconut oil and cholesterol, separately or in combination. The blood lipid content was assayed. Lipoproteins were separated into fractions by agarose electrophoresis. The aorta, heart and liver were investigated macro- and microscopically. The most pronounced changes in lipid metabolism appeared as a consequence of combined administration of coconut oil and cholesterol; they were most expressed in rabbits, less in guinea pigs, and most weakly in rats. Typical atherosclerotic lesions were observed only in rabbit's aorta. They were accompanied by a marked depression of the percentage content of alpha-lipoproteins in blood serum.

Animals↗