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Biomedical subjects

A Heimann

Publications and source records attributed to A Heimann.

67 records · Page 4Linked to original sources
PubMed1991

Determination of the rate of DNA synthesis of human tumor cells obtained by fine needle aspiration. Comparison of flow cytometry and an immunoperoxidase method for the detection of thymidine analogue incorporation.

Two methods of detecting thymidine analogue incorporation by lymphoma, leukemia and myeloma cells obtained by fine needle aspiration (FNA) are described. In one method, cells which have been incubated with the thymidine analogue iododeoxyuridine (IDURD) were exposed to primary monoclonal anti-IDURD antibody and a fluorescein-labeled linking antibody. The fluorescence of the antibody-labeled cells, which had synthesized DNA and incorporated the analogue, was detected by flow cytometry (FCM). In a second method, the cells that incorporated the analogue were detected on glass slide Cytospin preparations by an immunoperoxidase (IP) technique. The IDURD labeling index (LI), as determined by both FCM and IP staining, was compared to the percentage of cycling (S + G2/M) cells as determined by acridine-orange FCM. The data indicate that the IP method is reliable and correlated strongly with FCM determination of LI, percentage S-phase and lymphoma grade. Given the low cost and wide availability of IP technology, the IP method may be desirable for laboratories wishing to supplement cytology reports with cell cycle data.

Biopsy, Needle↗
PubMed1987

Hepatoblastoma presenting as isosexual precocity. The clinical importance of histologic and serologic parameters.

A 2-year-old boy presented with sexual precocity secondary to a hormone-producing hepatoblastoma. The tumor demonstrated an aggressive histologic pattern and stained immunohistochemically for alpha-fetoprotein and beta-subunit human chorionic gonadotrophin. After a successful liver transplant these tumor markers were closely followed. We conclude that histologic subclassification of hepatoblastoma is most useful for predicting likelihood of resectability. Tumors with predominantly fetal-type histology tend to be better circumscribed and are more likely to be resectable with improved survival relative to tumors with predominantly embryonal histology. The presence or absence of mesenchymal elements does not seem to have prognostic importance. Anaplastic features predict a poor outcome, but it remains for these to be uniformly defined and applied. Small uniform cellular histology with neuroblastoma-like differentiation appears to be particularly ominous. Serologic tumor markers, especially alpha-fetoprotein (AFP) and human chorionic gonadotrophin (HCG) are useful as monitors of potential tumor recurrence, but their efficacy is compromised by the unpredictable variation of tumor cell metabolism, undoubtedly a result of the genetic instability and heterogeneity of proliferating malignant cells.

Carcinoma, Hepatocellular↗
PubMed1986

Aspiration of the breast and nipple discharge cytology.

Between 1 January 1974 and 30 June 1984, 1,003 specimens obtained from the breast were submitted for cytologic evaluation to the Department of Pathology at the Waterbury Hospital Health Center. Eighty-eight per cent were obtained by fine needle aspiration of a palpable mass; the remainder were smears of nipple discharges. Precytologic and postcytologic clinical data were available for 80 per cent of the specimens. In the fine needle aspiration group, there were 219 instances of proved carcinomas; 79 per cent of these had either positive or suspicious cytologic findings. Seventy-five per cent of the patients with positive cytologic results underwent mastectomy without prior histologic confirmation of the needle aspiration and all were confirmed as malignant growths. There were 41 false-negative aspirates which were reviewed in detail. The cytologic slides of 24 of the 41 false-negative aspirates were available for re-evaluation by a second, independent cytopathologist. Only one was thought to have been misread initially; the remainder appeared to have been falsely negative for technical reasons related to aspiration or fixation technique or tumor size less than 1 centimeter. There was one false-positive finding in a patient who was found to have fat necrosis at biopsy. Results of nipple discharge cytology were less accurate. Positive or suspicious cytology was found in only five of 11 proved instances of carcinomas. There was one false-positive and five false-negative results in this group. We conclude that needle aspiration of palpable masses of the breast is an accurate, cost effective procedure which is readily adaptable to a community hospital, provided that a pathologist with an interest in cytology is available.

Adolescent↗
PubMed1983

Cushing's syndrome secondary to ectopic cortisol production by an ovarian carcinoma.

A 35-yr-old woman had Cushing's syndrome, amenorrhea, and elevated and nonsuppressible levels of urinary and plasma cortisol and urinary 17-hydroxycorticosteroids. An ovarian carcinoma with extensive metastases was found. Tumor concentrations of ACTH were much lower than those in any previously reported patient with the ectopic ACTH syndrome. Cortisol levels obtained from the venous effluent of tumor-involved ovaries were higher than that in peripheral plasma obtained at the same time. Transient postoperative normalization of plasma and urinary cortisol followed partial tumor removal. Electron microscopic study of the tumor revealed a cell type consistent with steroid-secreting cells. Postmortem examination revealed atrophy of both adrenal glands and no pituitary adenoma.

17-Ketosteroids↗
PubMedDate not supplied

On the number of measurements necessary to assess regional cerebral blood flow by local laser Doppler recordings: a simulation study with data from 45 rabbits.

Laser Doppler fluxmetry may improve the monitoring of cortical blood flow in neurosurgical patients. So far, however, the variability of laser Doppler readings found in the cerebral cortex has prevented a consequent usage of the technique in clinical practice. The current report analyzes the regional variability of laser Doppler readings from experimental animals. Typical frequency histograms of observed flow values display non-Gaussian distributions. A simulation technique is used to assess the number of measuring sites required for valid estimates of regional cortical flow. From a total of 990 local flow measurements from 45 rabbits random samples of sizes between 5 and 100 were repeatedly drawn to estimate the variability of median flow thus determined. The study underlines that small sample sizes below n = 20 are associated with a large variability (95th percentile: +38%) which decreases in a biphasic manner. Sample sizes above n = 25 are necessary to obtain more reliable information on regional cerebral blood flow: the 95th percentiles remain between 24 and 28% up to n = 45, and are still at 15% with n = 99.

Animals↗
PubMedDate not supplied

Measles cervicitis. Report of a case with cytologic and molecular biologic analysis.

We report on a 20-year-old woman who, in her third month of pregnancy, presented with a morbilliform rash, fever and diarrhea. Many multinucleate superficial, intermediate and metaplastic cells were noted in the cervical cytologic preparation. The presence of the measles virus in cells from the cervix was confirmed by the amplification of measles complementary DNA (cDNA) by the polymerase chain reaction. The patient's cervical Papanicolaou smear was unremarkable on reexamination four months after the onset of the disease. To our knowledge, this is the first verified reported case of infection of the uterine cervix by the measles virus.

Adult↗
PubMedDate not supplied

Spinal metastasis of a thyroglobulin-rich Hürthle cell carcinoma detected by fine needle aspiration. Light and electron microscopic study of an unusual case.

A prospective diagnosis of metastatic thyroid carcinoma was made on an aspirate of a spinal mass. While the cellular component of the aspirate was compatible with a renal or hepatic neoplasm, the recognition of colloid on air-dried smears defined the cells as Hürthle cells. Biopsy of the spinal tumor and subsequent thyroidectomy revealed a Hürthle cell carcinoma. Whereas the primary tumor showed typical Hürthle cell architecture, with solid tumor nests and microfollicle formation, the metastasis contained areas of macrofollicle formation with abundant colloid production and strong immunocytochemical reactivity for thyroglobulin. This change of histologic pattern from the primary tumor to the metastasis has not been previously reported in Hürthle cell lesions. The unusual light microscopic and ultrastructural aspects of this tumor are discussed.

Aged↗
PubMedDate not supplied

Carcinomatous meningitis as the initial manifestation of breast cancer.

Report is made of an elderly woman in whom carcinomatous meningitis was the initial manifestation of breast cancer. The patient presented with nonfocal neurologic symptoms. Cytologic examination of cerebrospinal fluid (CSF) revealed malignant cells arranged either in loose clusters or as isolated single cells. The morphology of the cells, some of which had a signet-ring configuration with a crescent-shaped nucleus as well as prominent round nucleoli, suggested a breast primary, which was proven by subsequent histologic study.

Aged↗
PubMedDate not supplied

Fine needle aspiration cytology of thymolipoma. A case report.

A case of thymolipoma in a 14-year-old girl diagnosed prospectively by fine needle aspiration (FNA) biopsy is reported. The cellular constituents of the aspirate, lymphocytes and epithelial cells, were characterized by routine cytology and immunocytochemistry. Although primarily based on the FNA cytology, the diagnosis was supported by the patient's clinical history and the radiographic demonstration of a fat density mass. This appears to be the first description of thymolipoma in a fine needle aspirate.

Adolescent↗
PubMedDate not supplied

Cytologic and histologic changes in irradiated loops of transplanted small bowel.

The histopathologic and cytopathologic changes in irradiated transplanted loops of bowel were assessed in 10 dogs. Five received conventional radiotherapy (CRT), and five received hyperfractionated doses of radiotherapy (HFRT) over the same period. Both groups exhibited identical cytologic findings, including blunting of villi and significant crypt atypia that could be mistaken for malignancy. Histologic examination corroborated the cytologic findings. At the end of the experiment two of the dogs that received CRT had developed strictures. One showed extensive submucosal fibrosis, while the other showed submucosal atrophy.

Animals↗