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Biomedical subjects

A Healy

Publications and source records attributed to A Healy.

At least 37 records · Page 2Linked to original sources
PubMed1993

Proteolytic specificity of chymosin on bovine alpha s1-casein.

The proteolytic specificity of chymosin (EC 3.4.23.4) on bovine alpha s1-casein at 30 degrees C in phosphate buffer, pH 6.5 and at pH 5.2 in the presence of 5% (w/v) NaCl was investigated. Peptides (pH 4.6-soluble) were isolated by reversed-phase HPLC and identified from their amino acid sequence; the identity of some peptides was confirmed by mass spectrometry and/or amino acid composition. The small peptides produced at pH 6.5 were Arg1-Phe23, Phe24-Phe28, Phe24-Leu40(?), Phe150-Phe153, Phe150-Leu156, Tyr154-Tyr159, Tyr154-Trp164, Asp157-Trp164 and Tyr165-Trp199. The same peptides, except Tyr154-Trp164, were produced at pH 5.2 in the presence of NaCl and, in addition, the peptides Arg1-Leu11, Phe24-Phe32, Lys102-Leu142, Ala143-Leu149 and Tyr165-Phe179. The rates of production of individual peptides differed under the two conditions studied but Arg1-Phe23 and Tyr165-Trp199 were the first and second peptides produced under both conditions. Pathways are proposed to interpret the proteolysis of alpha s1-casein in solution under the conditions of this study.

Amino Acid Sequence↗
PubMed1992

Participation by pediatricians in early intervention: impetus from Public Law 99-457.

Part H of the Individuals with Disabilities Education Act (originally enacted as Public Law 99-457) requires that participating states phase in a system of early intervention services by 1993. By recognizing the importance of good health in the development of infants and toddlers, Congress acknowledged the key role of medical care providers in a comprehensive program for young children with or at risk for developmental delay or dysfunction. National and state surveys of pediatricians suggest limited but growing awareness of this legislation and uncertainty about how they might participate effectively. A chief concern relates to mechanisms of payment for developmental screening and assessment as well as time-demands for participation in interdisciplinary team activities. The American Academy of Pediatrics and its state chapters are responding to requests for information with educational seminars and print materials. Pediatricians can enhance the quality of community support services for children with special needs by participating in planning efforts and by coordinating health care with other aspects of early intervention. Other professionals and parents are looking to pediatricians for leadership and willing participation in the implementation of PL 99-457.

Child↗
PubMed1988

Leukotriene D4-induced increases in the cytoplasmic pH of human myelocytic leukocytes.

The effects of leukotriene D4 on the intracellular pH of human myelocytes, derived from cultured HL-60 cells by dimethylsulfoxide-induced differentiation, were quantified with the fluorescent indicator 2',7'-bis-(2-carboxy-ethyl)-5,6-carboxyfluorescein. Leukotriene D4, but not C4 or E4, increased intracellular pH optimally by 3 min with a half-maximal effect at 1-2 nM. The increases in intracellular pH stimulated by leukotriene D4 were prevented by pretreatment of myelocytes with leukotriene D4 but not peptide chemotactic factors. Analogs of amiloride that inhibit selectively the Na+/H+ antiport also prevented the intracellular alkalinization induced by leukotriene D4. The rate of recovery of intracellular pH after an acid load with 30 mM sodium propionate was approximately 30% higher at each level of intracellular pH for myelocytes exposed to leukotriene D4 than for those challenged in buffer alone. The increase elicited by leukotriene D4 in the adherence of myelocytic leukocytes to surfaces thus is associated with an enhanced sensitivity of the Na+/H+ antiport to intracellular pH, that is, not coupled to an earlier rise in the cytosolic level of Ca+2.

Calcium↗
PubMed1983

Life outcomes and satisfaction ratings of multihandicapped adults.

Ten and 20 year longitudinal data were collected on 180 multihandicapped adults. Subjects completed a 50-item questionnaire which was divided into five sections: (a) living circumstances, (b) physical status, (c) social situation, (d) education, and (e) vocational training and employment. Respondents provided both outcome data and their satisfaction with those outcomes. The results indicated that most lived at home, were financially dependent and were not married. However, they reported that they were satisfied with these life outcomes and that they were very satisfied with their social activities. Few significant differences separated the age groups, suggesting that societal and legal changes over the last 20 years have had a limited impact. The variable most strongly related to life outcome and satisfaction with life outcome was functional status. In addition, perceived (self-reported) functional skills were highly correlated with more objective clinical ratings of functional status.

Activities of Daily Living↗