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Biomedical subjects

A Haddad

Publications and source records attributed to A Haddad.

At least 109 records · Page 6Linked to original sources
PubMed1994

[Bilateral infarction of the caudate nuclei].

We report the case of a 57-year-old right-handed woman, with a history of hypertension, who, in February 1990, suddenly developed behavioral and cognitive abnormalities. Prior to the onset of her illness she had been normal. On examination, neuropsychological testing (Wechsler Mental Test, Wechsler Adult Intelligence Scale Revised, Knox Cube Test) elicited attention abnormalities, decreased recent memory, apathy, reduced spontaneity and initiative and left hemiparesia. CT scan showed small low density areas in the head of both caudate nuclei and right internal capsule, indicating infarction. Two years later, the deficit had partially resolved. Apathy persisted; psychometry showed an IQ of 57. Bilateral damage to the head of the caudate nuclei disrupt cortical-subcortical connections. The caudate nucleus is an essential component of basal ganglia-thalamo-cortical circuitry and its contribution to cognitive functions and behavior appears to be important.

Caudate Nucleus↗
PubMed1993

Studies on the origin of the glycoproteins of the rabbit vitreous body using a protein-synthesis inhibitor and radioactive fucose and amino acids.

Rabbits were injected intravitreally with [3H]-fucose and were killed at 0.5, 4, 24, and 40 h postinjection. The radiometric measurements and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) plus fluorography performed on the vitreous bodies of these rabbits were compared with those carried out on excised vitreous bodies of noninjected rabbits incubated with [3H]-fucose for the same periods. The results of these in vitro experiments demonstrated that at all test intervals there were only two lightly labeled bands in the fluorograms, and this was interpreted as nonenzymatic binding of [3H]-fucose to proteins (glycation), occurring in the extracellular environment. In contrast, intravitreal injections resulted in about 14 conspicuously labeled bands, mainly after the longer time intervals, and the nature of these bands was revealed by additional experiments. In another series of experiments, rabbits were injected intravitreally with either [3H]-fucose or [3H]-amino acids and their vitreous bodies were processed as described above. The specific activity (counts per minute per microgram of protein) of the glycoproteins labeled with [3H]-fucose was always higher than that of the proteins labeled with any of the amino acids. In fluorograms, eight bands unequivocally labeled with the [3H]-amino acids were detected, and corresponding bands almost invariably appeared in the [3H]-fucose lane. Cycloheximide, a protein-synthesis inhibitor, blocked almost completely the incorporation of both [3H]-proline and [3H]-fucose into the vitreous proteins as revealed by the radiometric measurements and fluorography.(ABSTRACT TRUNCATED AT 250 WORDS)

Amino Acids↗
PubMed1992

Morphologic and molecular changes during the post-natal development of the rabbit vitreous.

Rabbits aged 1, 4, 10, 15, and 20 days, and 4 months were anesthetized and perfused with 4% formaldehyde. One eye of each rabbit was processed for paraffin embedding, while the other eye was embedded intact in methacrylate. Rabbits aged 1 and 15 days and 4 months were perfused with 2.5% glutaraldehyde, and the eyes were processed for Epon embedding. The paraffin sections were immunostained to allow detection of a high molecular weight cartilage matrix glycoprotein (CMGP), which is synthesized by the ciliary body and found in the vitreous in adult animals, using a specific mouse monoclonal antibody. CMGP was identified in the vitreous and in the inner layer of the ciliary epithelium only after the fifteenth day of life in amounts comparable to those detected in adult rabbits. Before this time immunostaining with the monoclonal antibody was seen only in the apical region of the inner ciliary epithelial cells. However, electron microscopic observations revealed that the cytoplasmic organelles responsible for the secretion of glycoproteins, i.e. the rough endoplasmic reticulum, Golgi apparatus, and vesicles, were present in the inner layer of ciliary epithelial cells as early as the first day of life. Anteroposterior sections of whole eyes embedded in methacrylate revealed a relatively dense meshwork of vitreous fibrils on the first day of life. The blood vessels were concentrated at the posterior region of the lens, and isolated cells were visible. The blood vessels were not seen after the age of 15 days, and the fiber meshwork and cells were inconspicuous by then.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗