Pattern of viphage types and emergence of resistance to fluoroquinolones in multi drug resistant S. typhi at high altitudes.
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Biomedical subjects
Publications and source records attributed to A Goyal.
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Severe upper gastrointestinal bleeding is unusual in newborns, and is usually seen in sick premature infants. We report on three healthy full-term neonates who, after uneventful deliveries, presented with profuse bleeding in the first 48 h of life. Two infants had duodenal ulcers and one had diffuse hemorrhagic gastritis. All three patients responded to conservative medical therapy and have shown no recurrence of symptoms during a follow-up period of 1-4 yr.
The knowledge about infant dental care (as a part of primary preventive programme) was delivered by the existing health team of CHC viz. medical doctors, multipurpose workers, health workers, Anganwadi workers (ICDS scheme), after due training from the dental experts, in the rural community of Raipur Rani (Haryana). The knowledge of community regarding infant dental care subsequent to intervention strategies when evaluated and compared to baseline values three years after intervention revealed that 72 percent of the community had the correct knowledge of prolonged breast/bottle feeding causing nursing bottle caries. 94 per cent had correct knowledge about harmful effects of thumb/finger sucking on teeth and jaw bones and 77 percent about harmful effects of mouth breathing. 98 percent of expecting mothers knew when to clean the gum pads and 62 percent how to clean the gum pads in an infant. 100 percent of the expecting mothers had the correct knowledge that pacifiers should not be used in small children.
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Unicellular green algae have a mechanism for concentrating dissolved inorganic carbon (DIC) only when grown in low CO(2). To find proposed transporter protein(s) for DIC, we isolated intact chloroplasts from Dunaliella tertiolecta cells, separated the chloroplast envelopes by isopyknic centrifugation, and separated their polypeptides by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Two peptides of apparent molecular masses of 45 and 47 kD were constituents of the inner chloroplast envelope only if the cells had been adapted to low CO(2) in the light or grown in low CO(2). These two low CO(2)-induced peptides appear to be part of the algal DIC pump.
Carbonyl sulfide (COS), a substrate for carbonic anhydrase, inhibited alkalization of the medium, O(2) evolution, dissolved inorganic carbon accumulation, and photosynthetic CO(2) fixation at pH 7 or higher by five species of unicellular green algae that had been air-adapted for forming a CO(2)-concentrating process. This COS inhibition can be attributed to inhibition of external HCO(3) (-) conversion to CO(2) and OH(-) by the carbonic anhydrase component of an active CO(2) pump. At a low pH of 5 to 6, COS stimulated O(2) evolution during photosynthesis by algae with low CO(2) in the media without alkalization of the media. This is attributed to some COS hydrolysis by carbonic anhydrase to CO(2). Although COS had less effect on HCO(3) (-) accumulation at pH 9 by a HCO(3) (-) pump in Scenedesmus, COS reduced O(2) evolution probably by inhibiting internal carbonic anhydrases. Because COS is hydrolyzed to CO(2) and H(2)S, its inhibition of the CO(2) pump activity and photosynthesis is not accurate, when measured by O(2) evolution, by NaH(14)CO(3) accumulation, or by (14)CO(2) fixation.
This study is a part of a larger preventive oral health feasibility module embarked on a population of 120,000 of Raipurrani, district Ambala (Haryana). The KAP analysis of about 2000 individuals (including school children) on cross-sectional basis at baseline, one and two years after the implementation of knowledge about oral health measures to the community utilizing the existing health infrastructure at CHC, the Anganwadi workers (ICDS Scheme) and through school teachers in the schools revealed that at baseline datun was the most prevalent (63%) oral hygiene measure used in the community and students. After implementation, the practice of brushing thrice, twice and once daily increased but the most prevalent frequency remained once a day (39% out of 66 percent using brush in community and 37% out of 76 percent in students after 2 and 11/2 years of implementation respectively) followed by twice a day (23 & 31% respectively). A similar pattern of frequency was observed about datun users also, though the number using datun substantially declined from 62% to 33% in community and 63 to 23% in students after implementation.
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3000 subjects from four high risk groups--patients attending STD clinics, hospital personnel, blood donors and foreign nationals were screened for the presence of HIV antibodies HBsAg and VDRL reactivity. No evidence of HIV infection was found in Himachiali population. However, there of the 150 foreign nationals screened were found positive for HIV antibodies. Overall HBsAg positivity and VDRL reactivity was found to be 5.53 per cent and 9.07 per cent respectively. The importance of circumspected and unswerving machinery for the surveillance of HIV antibodies in the high risk population of the state is emphasised.
The KAP survey on oral health practices of 3247 individuals, 6-60 years of age, of Sidhaura block of Haryana revealed that use of dattan was more prevalent (56% in adult community & 49% students) than tooth brush (35% adult community & 34% students). 37% of the total community had the knowledge of brush being the best oral hygiene measure. 25% of the community using brush were brushing only once/day. The knowledge of the community regarding the role of fluorides in prevention of dental caries was completely lacking: 1.8% of the community was using fluoride dentifrice. 35-45 percent of the community were practising sweet foods/drinks etc. four times a day. The snyder test showed a positive relation with frequency of sugar intake.
Serum samples from 2645 individuals representing all the twelve districts of Himachal Pradesh were screened for antibodies to Human immunodeficiency virus (HIV), employing ELISA test. These were categorised into four major high risk groups, viz, patients attending STD clinics (1305), voluntary blood donors (1012), hospital staff dealing with blood and blood products (200) and foreign nationals (128). No evidence of HIV infection was found in Himachali population. Two foreign nationals were repeatedly found positive for HIV antibodies by ELISA test and these were confirmed by Western Blot technique.