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Biomedical subjects

A Gogate

Publications and source records attributed to A Gogate.

At least 37 records · Page 2Linked to original sources
PubMed1990

Microbiological study of urethritis in men attending a STD clinic.

A microbiological study of 275 male patients suffering from urethritis and 100 healthy male controls showed that Neisseria gonorrhoeae (130), Ureaplasma urealyticum (81), Staphylococcus aureus (38), and alpha and beta streptococci (34) were the common isolates. Specificity and sensitivity of the direct fluorescent antibody technique in the detection of N. gonorrhoeae in 130 urethral samples, were found to be 100 per cent. Penicillin (10 units/disc) resistance was found in 36.93 per cent of N. gonorrhoeae. Minimum inhibitory concentration of penicillin for 75 isolates of N. gonorrhoeae (including 5 beta lactamase producers) varied from 0.01-5 micrograms/ml with a 95 per cent confidence limit range of 0.26-0.61 microgram/ml. Most of the N. gonorrhoeae isolates tested were sensitive to norfloxacin and spectinomycin. Inclusion bodies of Chlamydia trachomatis were observed in 25 patients.

Adolescent↗
PubMed1990

Enzyme linked immunosorbent assay for immunoglobulins G to Mycoplasma hominis.

Sera (187) from women patients attending the STD, Obstetrics and Gynaecology, and Family Planning Clinics were screened for the presence of the IgG class of immunoglobulins against M. hominis, using the ELISA technique. Sonicates of locally isolated M. hominis serotype (CS1) and standard PG 21 strain were used as antigens. The test was standardized using penicillinase as an enzyme. The ELISA showed 90.48 per cent sensitivity and 84.8 per cent specificity, and was also rapid (as compared to culture) and reproducible.

Enzyme-Linked Immunosorbent Assay↗
PubMed1990

Mycoplasma hominis infections in female genital tract & use of immunofluorescence for antibody detection.

A total of 325 women with genital tract infection and 108 healthy controls were screened for the presence of mycoplasmas. Of these, 325 patients, mycoplasmas were recovered in 186. Thirty five isolates were M. hominis and 151 isolates of Ureaplasma urealyticum. The fluorescent-antibody technique (FAT) has been employed for the rapid identification of mycoplasma colonies growing on agar plates. The method was found to be rapid for detection of M. hominis growing on primary isolation plates. The antibody titre of patient's serum was also detected by FAT. Patients' sera were diluted four fold from 1:4 to 1:256 dilutions. In 7 patients fluorescence was seen with 1:16 serum dilution, in 18 with 1:64 dilution and in 3 with 1:256. The immunofluorescence test gave a sensitivity of 96.66 per cent, specificity of 95.0 per cent, with percentage of false negativity and false positivity being 3.45 and 5.0 per cent respectively.

Antibodies, Bacterial↗
PubMed1989

Co-existence of N. gonorrhoeae and U. urealyticum in male urethra.

Eight hundred and forty male patients attending sexually transmitted disease (STD) clinic for urethritis were investigated. Out of them, 31.6% had gonococcal urethritis, 16.1% suffered from nongonococcal urethritis due to Ureaplasma urealyticum and in 12.6%, both the organisms were present. Though 14.62% strains of N. Gonorrhoeae were resistant to penicillin, all the strains were sensitive to spectinomycin; while all Ureaplasma strains were sensitive to tetracyclines. As the treatment differs for these two organisms, it is necessary to identify the correct etiological agent.

Adolescent↗