Search PubMed⌕ Search

Biomedical subjects

A Giorgio

Publications and source records attributed to A Giorgio.

At least 73 records · Page 4Linked to original sources
PubMed1984

Monoclonal antibody testing of lymphocytes after overnight storage.

Often monoclonal antibody testing of lymphocytes is not performed until the day after blood collection for reasons of convenience or due to the need to transport the blood to other facilities. In order to determine whether accurate results can be obtained on the day after collection, we compared results obtained after storage overnight at 4 degrees C or 22 degrees C with results obtained with fresh lymphocytes. Lymphocytes from 24 normal individuals were evaluated with 10 monoclonal antibodies using an immunofluorescence technique with analysis by flow cytofluorometry. There were markedly altered results obtained with lymphocytes separated on the day after collection from whole blood stored at 4 degrees C. Lymphocytes separated from whole blood stored at 22 degrees C showed moderate changes in reactivity with some monoclonal antibodies. Lymphocytes that were separated from fresh blood and then stored at 4 degrees C or 22 degrees C showed results similar to fresh lymphocytes. These results underscore the importance of proper processing of blood samples to avoid misinterpretation of results.

Antibodies, Monoclonal↗
PubMed1982

[Hematologic diagnosis using fluorescence].

The authors have used a fluorescent supravital staining technique that allow the simultaneous study of platelets, the fibrin meshwork, the morphology of white blood cells and its phagocytic activity, the presence of immature cells. The technique is simple and fast and owing to all the information that it furnish, the authors designated it with the initials FED.

Acridine Orange↗
PubMed1978

[The use of electropherogram in a clinical setting (author's transl)].

The AA. have spent several years working on the correlation between EPG patterns and diagnosis upon discharge. They then attempted to codify those abnormalities which were statistically related to different illnesses. This codification should not be considered definitive; however, it can be suggestive of further studies especially for those hospitals equipped with a computer which can correlate EPG patterns with the initial diagnosis or the diagnosis on discharge.

Biliary Tract Diseases↗
PubMed1978

[A new method of cytological staining].

The haematological method (Giemsa, Pappenheim, Wright, ecc.) is renowed for karyological legible, while the cytologic method (Papanicolau, Shorr, haematoxylon-eosine, etc.) for its view of the whole. Antithesis between two methods was subject polemics and discussions after put in round table promoted by Acta cytological in 1958. The present work suggests for the attention of cytologists an attempt at technical reconciliation of two methods, it obtained with a new coloration method that uses like fixative a cadmiumalcoholacetic mixture, like nuclear colouring the basic fuchsine maturing in presence of formic aldehyde and acetic acid, like cytoplasmatic colouring a triacid orange-eosine-blue mixture of aniline.

Bone Marrow Diseases↗
PubMed1976

[A proposed hepatic pie chart].

The AA. have chosen 12 tests to organize an "hepatic flash" after a selection out of 180 cases scheduled as the table no. I through a set of tests showing step by step any modifications in compliance with the sensibility, reliability, statistical clinical and physio-pathological meaning of each test. The 12 tests are set out by circular abscissae of a circus according to the pathogenetic mechanisms leading to their modifications. By the radial ordinates, instead, it is shown the decreasing and increasing values which every test may assume. An hypothetic line (hepatogram) links then the values set out during the process of the disease in such a manner that the particular shapes assumed by the line itself show quickly and visually the spreading rate of the disease process.

Bilirubin↗
PubMedDate not supplied

Transabdominal sonography of the normal gastroesophageal junction in children.

PURPOSE: Because sonography identifies abnormalities of the gastroesophageal junction, it is essential to understand the normal sonographic anatomy. The aim of this study was to determine the normal sonographic appearance of the gastroesophageal junction and its variations and to provide measurements of the abdominal esophagus in asymptomatic, healthy children. METHODS: In this prospective study, 124 healthy children (75 boys and 49 girls), aged 2 days-12 years, underwent abdominal sonography. With the patient in a supine position, the transducer was placed under the xiphoid and the ultrasound beam was directed cephalad through the window of the left lobe of the liver. The length of the abdominal esophagus was measured from the point at which it penetrated the diaphragm to the gastroesophageal junction. The thickness was measured on the anterior wall at the midpoint of the abdominal esophagus. RESULTS: The gastroesophageal junction was identified by sonography in all of the children. The mean length of the abdominal portion of the esophagus ranged from 18 mm in the newborns to 34 mm in children older than 6 years. The wall thickness ranged from 2.4 mm to 5.7 mm. CONCLUSIONS: Our results indicate that visualization of the gastroesophageal junction and measurement of the abdominal esophagus are readily achievable with real-time sonography in healthy children.

Abdomen↗
PubMedDate not supplied

Ultrasound evaluation of uncomplicated and complicated acute viral hepatitis.

Seven hundred ninety-one consecutive patients with acute viral hepatitis, 17 of whom had liver failure, and 97 healthy volunteers were examined by ultrasound. No specific patterns were found in either the uncomplicated or the complicated forms. Only 19 subjects showed a typical "bright liver" pattern, which is correlated with significant vacuolar hepatocellular degeneration. The increased brightness and clear visualization of portal vein radicle walls, previously described in this disease, were detected in only 32.2% of the hepatitis patients but were also seen in 30.9% of the normal controls.

Acute Disease↗
PubMedDate not supplied

One-shot percutaneous ethanol injection of liver tumors under general anesthesia: preliminary data on efficacy and complications.

PURPOSE: To verify the efficacy of ultrasound (US)-guided injection of large amounts of ethanol into large or multiple liver lesions, in a single session under general anesthesia (one-shot PEI) for percutaneous ablation of hepatic tumors. METHODS: Twenty-nine patients (27 with 51 hepatocellular carcinoma (HCC) nodules on cirrhosis, diameter range 1.0-9.0 cm; two patients with a single metastasis from the gastroenteric tract, 5.0 and 9.0 cm, respectively, in diameter) were treated with one-shot PEI. RESULTS: The total volume of alcohol delivered per patient ranged from 16 to 210 ml. Mean ethanol volume in all patients was 49 ml. Dynamic computed tomography (CT) examination showed complete necrosis in 41 of 50 lesions. Two patients died of hypovolemic shock due to massive upper gastrointestinal bleeding, 3 and 7 days, respectively, after the interventional procedure. All the remaining patients are alive (follow-up 5-14 months) except one who died of liver failure 5 months after. New HCC nodules occurred in six patients within 6 months and one intralesional relapse was recorded. CONCLUSION: In this preliminary experience, one-shot PEI is as effective in inducing liver tumor necrosis as traditional PEI; its advantages are shorter treatment time and the capability of treating larger and multiple liver lesions.

Aged↗
PubMedDate not supplied

Disseminated mycobacterial infection in AIDS patients: abdominal US features and value of fine-needle aspiration biopsy of lymph nodes and spleen.

BACKGROUND: We evaluated the efficacy of abdominal ultrasound (US) and fine-needle aspiration biopsy (FNAB) in the diagnosis of disseminated mycobacteriosis (DM) in acquired immunodeficiency syndrome (AIDS). METHODS: Twelve AIDS patients (nine male, three female; age range, 22-43 years) with DM underwent abdominal US within 2 days after admission with 3.5- to 5-MHz convex probes and a 7.5-MHz linear probe. All patients underwent FNAB of one or two enlarged abdominal lymph nodes. Eight patients underwent FNAB of the spleen. The aspirated specimens were stained with acid fast for quick examination and cultured for isolation of mycobacteria. RESULTS: Abdominal US showed enlarged, hypoechoic, round or oval, abdominal lymph nodes (diameter, 10-35 mm; mean, 18 mm) in all patients; splenomegaly (spleen diameter, 14-22 cm; mean, 16.8 mm) in all patients; numerous splenic abscesses (diameter, 3-20 mm) in nine patients; hepatomegaly (right hepatic lobe thickness, 14.5-17 cm) in all patients; small intestinal wall thickening in five patients (maximum bowel wall thickness, 7-15 mm); mild to moderate ascites in six patients; pleural effusion in four patients; bilateral enlargement of the kidneys with hyperechogenicity of the cortex in three patients; and a retroperitoneal tubercular abscess in one patient. No complication occurred after FNAB of lymph nodes and spleens. Fast-acid stain of spleen and/or lymph node FNAB specimens allowed early diagnosis of mycobacteriosis in 12 of 12 cases (100%). Cultures of lymph node aspirates grew mycobacteria in six of 12 patients (50%). Spleen aspirates grew mycobacteria in nine of nine patients (100%). Blood cultures were positive in four of 12 patients (33%). Mycobacterium tuberculosis was diagnosed in six patients and M. avium in five. CONCLUSION: Abdominal US features can suggest DM in AIDS patients. Spleen and/or lymph node FNAB indicated the specific diagnosis in 100% of patients.

AIDS-Related Opportunistic Infections↗
PubMedDate not supplied

Diagnosis of benign and malignant portal vein thrombosis in cirrhotic patients with hepatocellular carcinoma: color Doppler US, contrast-enhanced US, and fine-needle biopsy.

BACKGROUND: We assessed the role of contrast-enhanced ultrasound (CEUS) in the differential diagnosis between benign and malignant portal vein thrombosis in patients who had cirrhosis with hepatocellular carcinoma (HCC). METHODS: Fifty-four consecutive patients who had cirrhosis, biopsy-proved HCC, and thrombosis of the main portal vein and/or left/right portal vein on US were prospectively studied with color Doppler US (CDUS) and CEUS. CEUS was performed at low mechanical index after intravenous administration of a second-generation contrast agent (SonoVue, Bracco, Milan, Italy). Presence or absence of CDUS signals or thrombus enhancement on CEUS were considered diagnostic for malignant or benign portal vein thrombosis. Twenty-eight patients also underwent percutaneous portal vein fine-needle biopsy (FNB) under US guidance. All patients were followed-up bimonthly by CDUS. Shrinkage of the thrombus and/or recanalization of the vessels on CDUS during follow-up were considered definitive evidence of the benign nature of the thrombosis, whereas enlargement of the thrombus, disruption of the vessel wall, and parenchymal infiltration over follow-up were considered consistent with malignancy. CDUS, CEUS, and FNB results were compared with those at follow-up. RESULTS: Follow-up (4 to 21 months) showed signs of malignant thrombosis in 34 of 54 patients. FNB produced a true-positive result for malignancy in 19 of 25 patients, a false-negative result in six of 25 patients, and a true-negative result in three of three patients. CDUS was positive in seven of 54 patients. CEUS showed enhancement of the thrombus in 30 of 54 patients. No false-positive result was observed at CDUS, CEUS, and FNB. Sensitivities of CDUS, CEUS, and FNB in detecting malignant thrombi were 20%, 88%, and 76% respectively. Three patients showed negative CDUS and CEUS and positive FNB results; follow-up confirmed malignant thrombosis in these patients. One patient showed negative CDUS, CEUS, and FNB findings. However, follow-up of the thrombus showed US signs of malignancy. Another FNB confirmed HCC infiltration of the portal vein. CONCLUSION: CEUS seems to be the most sensitive and specific test for diagnosing malignant portal vein thrombosis in patients with cirrhosis.

Aged↗
PubMedDate not supplied

[Syndrome of the "Lamblia intestinalis" (author's transl)].

The Authors related the physiopathologic remarks executed on subjects that are suffering from Lambia intestinalis. They have been chisen in a group of 226 individuals who had executed the coprologic test because of symptoms of gastroenteric apparatus. With the escort of this observation, the Authors have findings, as constant report, a typical enteritis, that reveals itself in periodical diarrhoea, alternated with constipation and often accompanied, in the children, with gastralgia, in the grown ups with duodenitis. The constancy of these reports and the therapeutical succes, whic the have been achieved in the most of the cases with antilambliasi, induce them to have reasonable motives to attribute to the Lamblia a definite pathogenus entity and to regard it an etiologic element of the above-mentioned unhelty courses.

Adolescent↗
PubMedDate not supplied

[Biology of the "Salmonella wien" (author's transl)].

The Authors after a theoric introduction in regard to the taxonomic position, the history and the epidemiology of the Salmonella wien, explain the biologic characteristic of the bacterium according to results of their experiments.

Anti-Bacterial Agents↗