Search PubMed⌕ Search

Biomedical subjects

A German

Publications and source records attributed to A German.

At least 37 records · Page 2Linked to original sources
PubMed1987

Relation between protective potency and specificity of antibodies in sera of tetanus immunized individuals.

Out of 157 human sera analyzed for antitetanus antibody content by ELISA, 13 turned out to contain only anti-BIIb antibodies, of which 8 proved to be neutralizing. Of these, the 3 sera 303, 306 and 312 together with a commercially available standard preparation of human antitetanus immunoglobulins were further analyzed as to their antibody composition by ELISA using plates sensitized with either the toxoid or various tetanus toxin-derived fragments. It was verified that the protective potency of these antisera was related mainly to their anti-BIIb antibody content. Adsorption experiments confirmed that anti-BIIb antibodies were primarily involved in toxin neutralization, although the presence of high levels of both anti-alpha and anti-Ibc antibodies could confer neutralizing capacity on the sera. A rabbit antiserum raised with the BIIb fragment resulted in a neutralizing antiserum that allowed us to calibrate the ELISA with the anti-BIIb antibodies as International Units.

Adolescent↗
PubMed1987

Immunity to tetanus: tetanus antitoxin and anti-BIIb in human sera.

Immunity to tetanus was investigated in 157 individuals aged between 1 and 77 years using, for the evaluation of both tetanus antitoxin and antibody to fragment BIIb (anti-BIIb), an easily performed ELISA that gave reproducible results. Among these people 72% were protected against tetanus (tetanus antitoxin titres greater than 0.06 IU ml-1). Anti-BIIb titres greater than 0.15 U ml-1 were found in 75% of the males vs 57% of the females (P less than 0.02) with marked variations according to age. Furthermore, 13 out of 41 individuals with antitoxin titres less than 0.06 IU ml-1 (not protected against tetanus) were found to have anti-BIIb titres greater than 0.15 U ml-1. These data raise questions as to the significance and protective effect of anti-BIIb against tetanus.

Adolescent↗
PubMed1987

Role of interferon, antibodies and macrophages in the protective effect of Corynebacterium parvum on encephalomyocarditis virus-induced disease in mice.

Treatment of mice with Corynebacterium parvum enhances their resistance to encephalomyocarditis (EMC) virus infection. In EMC-virus-infected mice, pretreated with C. parvum, neither interferon production nor antibody responses were increased. A decrease of virus recovery was observed in cultures of macrophages taken from the peritoneum of mice early after injection of C. parvum and infected with EMC-virus in vitro. The data suggest that C. parvum acts by increasing intrinsic antiviral activity of macrophages.

Animals↗
PubMed1987

Inhibition of [14C]aminopyrine uptake in rat isolated gastric mucosal cells by two classes of psychotropic agents.

Certain CNS-active compounds decrease gastric acid secretion in vivo. In this study a number of tricyclic antipsychotic or antidepressant compounds together with haloperidol, a nontricyclic antipsychotic agent, were shown to inhibit dibutyryl-cAMP-stimulated [14C]aminopyrine uptake, an index of acid secretory activity in a rat isolated gastric mucosal cell preparation. The observed order of potency was: thioridazine greater than chlorpromazine greater than haloperidol approximately equal to desipramine approximately equal to imipramine greater than clozapine. Comparison of these potencies with those of the known (H+ + K+)ATPase inhibitors timoprazole and omeprazole revealed that the potency of timoprazole was similar to the one of clozapine while omeprazole was intermediate between thioridazine and chlorpromazine. Pirenzepine was ineffective.

2-Pyridinylmethylsulfinylbenzimidazoles↗
PubMed1986

[Effect of the administration of killed Lactobacillus acidophilus on the survival of suckling mice infected with a strain of enterotoxigenic Escherichia coli].

After oral administration of 10(2) to 10(5) CFU of Escherichia coli B41 (0101:K 99+:ST+) to 24-48 h old suckling mice (Swiss OF1), a 80 to 100% mortality rate is observed within three days. We compared the effect of the oral treatment with a lyophilized preparation of heat-killed Lactobacillus acidophilus and with sterile water on the mortality rate of newborn mice. In six out of seven assays, the heat-killed L. acidophilus administration extended survival of infected suckling mice (P ranging from 0.019 to less than 0.001). In another test, result was contradictory. A global analysis indicated that the two treatments were statistically different (P less than 0.001). Lactic acid was unable to induce protection.

Animals↗
PubMed1983

Influence of inhaled cadmium microparticles on mouse influenza pneumonia.

A study of the effect of inhaled cadmium microparticles (CdO) on the mouse death rate from influenza pneumonia was performed on 936 female specific pathogen-free (SPF) Swiss mice. The test animals received a single short (15 min) exposure to 9 mg Cd/m3 of air (acute exposure), or renewed short exposures to 1 mg/m3 once a day, 5 days a week, for 4 weeks (chronic exposure). The cadmium found in the trachea-bronchus-lung area was about 5 micrograms/g of fresh tissue at the end of acute exposure, and 4 micrograms/g at the end of chronic exposure. The viral challenge was performed 48 hr after acute exposure, or on the 14th day after the beginning of chronic exposure, the mice being reexposed to Cd for an additional 14 days in the latter case. Surprisingly, the infectious death rate of test mice was significantly lower than that of controls, both for acute and chronic exposure to CdO. These results are discussed.

Aluminum Oxide↗
PubMed1983

[Value of C-reactive protein in the cardiac postoperative period].

Twenty patients underwent special clinical and biological monitoring during their period of hospitalisation in the Department of cardiovascular surgery. The clinical monitoring concentrated on the patient's temperature and the search for postoperative complications; the biological part of the study was concerned with monitoring the levels of serum C-Reactive protein (CRP) as assessed by an immuno-nephelometric method. In 10 patients with a normal postoperative course the levels of this protein, an indicator of an inflammatory or infective process, were similar, giving an identical graph in all patients. On the other hand, in the 10 patients with inflammatory or infective postoperative complications, the levels of CRP were abnormal, parallel with the clinical state, sometimes rising even before the complication manifested itself clinically. It therefore seems useful and justified to measure CRP systematically, once before surgery and at least once daily in the postoperative period. The frequency of this investigation could be increased in patients with difficult or complex postoperative courses. A high CRP, even with a normal temperature, should alert the surgeon to a complication or to the inefficacy of anti-inflammatory or anti-infective therapy. These results confirm those published by other surgical teams, both in cardiovascular surgery and traumatology.

C-Reactive Protein↗
PubMed1982

The effect of alcohol ingestion on the susceptibility of mice to viral infections.

The influence of acute alcoholization on the evolution of different viral diseases was studied in orally alcoholized mice. Ethanol increased mice susceptibility to encephalomyocarditis and influenza only when it was administered after virus infection. There was no dose-effect correlation. Vaccinia and Herpes virus infections were not modified by alcoholization. Hypotheses concerning the mechanisms of alcohol action were put forward.

Animals↗