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Biomedical subjects

A Gaulier

Publications and source records attributed to A Gaulier.

At least 37 records · Page 2Linked to original sources
PubMed1993

[Mesothelial cell. Cytology. Cytochemistry. Immunocytochemistry. Contribution of immunolabelling to the cytodiagnosis of serous effusions with cells of mesothelial aspect].

This paper reviews the contribution of cytochemical and immunocytochemical methods to the cytodiagnosis of serous fluids. For this purpose, 1000 fluids were reviewed (500 pleural and 500 peritoneal samples). The most reliable histochemical technique is the iron stain which labels macrophages. A minimum immunolabelling panel (including one or two epithelial markers and vimentin), applied to the identification of the usual phenotypes of reactive fluids with mesothelial cells or with macrophages, to adenocarcinomatous fluids and to malignant mesothelioma fluids, is proposed. The sensitivity and specificity of the reaction is discussed but, altogether the value of the method appeared rather limited as a correct diagnosis was obtained in 95% of cases by a careful morphological study.

Adenocarcinoma↗
PubMed1993

[Pancreatic insulinoma, adenomatosis of the Wirsung's duct and chronic pancreatitis. Apropos of a case].

The clinical investigations carried out in a 58 years woman complaining of malaise led to the discovery of an hypoglycaemia resulting from a secreting pancreatic insulinoma. In addition, a chronic pancreatitis, an endocrine hyperplasia (possible nesidioblastosis) and a villous adenomatosis of the pancreatic duct were diagnosed on two biopsies. The immunohistological tests performed on the insulinoma showed insulin, calcitonin and gastrin labelled cells. Electron microscopy displayed numerous neurosecretory granules. The peritumoral endocrine hyperplasia contained intermingled B, A and D cells respectively labelled by insulin, glucagon and somatostatin. Following the operation, the patient recovered without recurrence of the hypoglycaemia (three year follow-up). Factors which may explain such a rare pathological association are discussed.

Adenoma, Villous↗
PubMed1993

[Squamous erythema annulare centrifugum].

A case of squamous erythema annulare centrifugum has been observed. It was characterized by striking clinical features and peculiar histological findings, including necrosis of keratinocytes. A search for causative factors was negative. Treatment with tar ointment (Brocq) rapidly resulted in dramatic healing of the lesions; however, the usual features of annular psoriasis were absent in our case.

Erythema↗
PubMed1993

[Inclusion of microfilaments and conventional cytology. Two complementary techniques applied to products of puncture with fine needle].

A technique has been developed to complete the processing of fine needle aspiration biopsy material of deep and/or superficial organs: a method of collection and "prefixation staining" allowing visualisation of very small coherent fragments (microfragments) obtained by fine needle aspiration biopsy and which can consequently be submitted to histological examination. This method optimises CT-guided fine needle aspiration biopsies which, up until now, were only submitted to cytological examination (smears on slides, cell pellets, concentration and cytocentrifugation). Histological analysis of microfragments of 35 aspiration biopsies of various organs (pancreas, liver, adrenals, retroperitoneal nodes, paravertebral and mediastinal masses, etc.) was contributive in 71.4% of cases (25/35). In conclusion, the combination of cytological and microfragment inclusion techniques increased the diagnostic yield to 80% of cases.

Adenocarcinoma↗
PubMed1992

[Lipomas of the mesentery in young children].

Lipomas of the small bowel and mesentery are exceedingly rare in young pediatric patients. This article reports a case which underlines the difficulty of establishing this diagnosis before exploratory laparotomy.

Child, Preschool↗
PubMed1992

[Malignant intravascular lymphoma (malignant angioendotheliomatosis), with adrenal localization. Report of a case].

A past history of epilepsy and terminal dementia in a fifty five year old patient lead to the post mortem diagnosis of malignant intravascular lymphoma (malignant angioendotheliomatosis). There was a scattered involvement of capillaries and venulae in leptomeninges, peripancreatic and perirenal adipose tissue, pericardium, myocardium. The only adrenal involvement was typically tumoral at macroscopic examination. Such a localization, often reported in B intravascular malignant lymphoma, is studied according to the recent publications of this entity.

Adrenal Gland Neoplasms↗
PubMed1991

Do measles early giant cells result from fusion of non-infected cells? An immunohistochemical and in situ hybridization study in a case of morbillous appendicitis.

A case of acute appendicitis with features of measles inflammatory reaction is studied. Two types of lymphoid polykaryons are seen: Warthin Finkeldey cells inside germinal centres (LN1 ++, LN2 +, L26 +, MB1 +, MB2 +/-) and multinucleate plasma cells in the lamina propria (mu +, alpha ++, kappa +, lambda +). Both types of polykaryon are devoid of inclusions. The search for viral genetic information by in situ hybridization was negative in these cells. A positive signal was observed in interfollicular mononuclear cells and rare enterocytes. A possible mechanism of fusion from without, acting at the beginning of the disease to induce the appearance of polykaryons, is discussed.

Acute Disease↗
PubMed1990

Tamm Horsfall protein expression by a small renal cell carcinoma presenting with metastases.

The clinical presentation of cervical metastases in a young woman presenting with cervical lymphadenopathy is described. The clinical, histological, ultrastructural and immunological findings establishing the diagnosis of a renal primary tumour are seen. The significance of mixed tumour cell expression of antigens specific for the proximal tubule (CD10, DPP4 and aminopeptidase N) and of Tamm Horsfall protein, normally expressed on the thick ascending limb of loop of Henle and distal tubule, is discussed.

Adult↗
PubMed1989

[Initial immunolabelling in the diagnosis of solid tumors undergoing cytologic studies (cytopuncture and imprints)].

A minimum immunocytolabelling panel was applied to cytologic specimens from 112 solid tumors obtained by operative specimen imprints or fine needle biopsies of superficial or deep organs. A confirmatory histopathologic study was performed subsequently in all cases. Cases were divided into two groups: group 1 (n = 69) included the malignant tumors exhibiting diagnostic morphologic features; this group served as a control. Group 2 (n = 43) included the undifferentiated malignant tumors that were not classifiable on the basis of routine cytomorphologic data. Immunocytolabelling was done using immunofluorescence and/or PAP and a minimum panel of monoclonal antibodies against keratin (KL1), epithelial membrane (EMA), vimentin, and leukocyte common antigens. In the overwhelming majority of cases, immunocytolabelling provided useful diagnostic information (carcinomas/malignant lymphomas, carcinomas/malignant melanomas, carcinomas/sarcomas, etc...). Some tumors simultaneously expressed an epithelial antigen and a vimentin-like antigen. The problem of such coexpression is discussed. In our opinion, immunocytolabelling of cytologic samples is especially useful for studying needle biopsy specimens of organs, particularly deep organs.

Diagnosis, Differential↗
PubMed1988

[A combination of mycosis fungoides and chronic myeloid leukemia. Apropos of a case].

The coexistence of a T-cell lymphoma with a myelodysplatic syndrome seems to be exceptional. In the case reported here the diagnostic problems raised by the appearance of cutaneous nodules in a patient with chronic myeloid leukaemia (CML) were solved by histo-immunological examinations. A 70-year old male patient had been presenting since 1976 with a psoriasis-like skin disease. He was first seen at the Argenteuil hospital in 1984. Physical examination showed psoriasiform finger-like erythemato-squamous lesions, infiltrated plaques and an ulcerated tumoral swelling of the right elbow. A diagnosis of mycosis fungoides was made on histological and immunological examination results. At histology, this epidermotropic lymphoma was peculiar in that the atypical infiltrate was clearly centred on vessels. Electron microscopy confirmed that the vascular walls were invaded by the mycosis cells. Additional examinations showed hyperleucocytosis and myelaemia which were rapidly attributed to a chronic myelocytic leukaemia since the Philadelphia chromosome was present and the leucocytes had a low alkaline phosphatase score. Bone marrow biopsy disclosed a myeloproliferative syndrome of the CML type. Biopsy of a right axillary lymph node showed myelocytic infiltration associated with dermopathic lymphadenitis. There were no circulating Sezary cells, and a search for extension proved negative. From May, 1984 to June, 1985 the patient's CML was treated with busulfan which produced blood and bone marrow remission. The skin lesions were treated first with mechlorethamine, then with topical corticosteroids. Superficial electron therapy was applied to the tumoral lesions.(ABSTRACT TRUNCATED AT 250 WORDS)

Aged↗
PubMed1987

Lethal chondrodysplasia punctata, Conradi Hünermann subtype A, one case.

Lethal Conradi Hünermann disease is described in a patient with severe skin and skeletal involvement. Skin lesions, now considered as a peculiar form of ichthyosis, consisted of adnexal and pilar follicle hyperkeratosis, polymorphonuclear exocytosis, irregular keratohyaline distribution and frequent paranuclear vacuoles in stratum Malpighi. Numerous chondrocyte clusters and calcified masses were found in the epiphyseal cartilage. The growth plate was disorganised. There was a bilateral cataract. This severe lethal form of Conradi Hünermann disease corresponds to the subgroup A denomination of Spranger and may be a severe manifestation of X-linked Chondrodysplasia punctata.

Bone and Bones↗