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Biomedical subjects

A G Taylor

Publications and source records attributed to A G Taylor.

At least 19 recordsLinked to original sources
PubMed2006

Biomarkers and potential mechanisms of obesity-induced oxidant stress in humans.

OBJECTIVE: Oxidative stress may be the unifying mechanism underlying the development of comorbidities in obesity. Evidence suggests that a clustering of sources of oxidative stress exists in obesity: hyperglycemia, hyperleptinemia, increased tissue lipid levels, inadequate antioxidant defenses, increased rates of free radical formation, enzymatic sources within the endothelium, and chronic inflammation. METHOD: This review provides a summary of the available evidence on systemic oxidative stress in humans and specific metabolic pathways by which obesity may elevate systemic oxidant stress. The authors suggest possible methods of reducing oxidative stress such as antioxidant supplementation, caloric restriction and/or physical activity and surgical intervention to combat free radicals and reduce adipose tissue. RESULTS: Obesity is associated with oxidative stress and can be reduced with weight loss (regardless of exercise or surgery induced weight loss), caloric restriction or antioxidant rich diets. CONCLUSION: Oxidative stress levels are elevated in human obesity, and these levels are modifiable with various lifestyle modifications and surgical interventions.

Antioxidants↗
PubMed2002

Paget's disease complicated by hydrocephalus and syringomyelia.

The authors report a case of Paget's disease involving the skull with the rare but severe complications of hydrocephalus and syringomyelia related to basilar impression. Complications of Paget's disease of the skull are briefly discussed and current treatment strategies outlined.

Cervical Vertebrae↗
PubMed2001

Advantages of delayed VP shunting in post-haemorrhagic hydrocephalus seen in low-birth-weight infants.

The optimal timing of ventriculo-peritoneal (VP) shunt insertion in a neonate with post-haemorrhagic hydrocephalus (PHH) is uncertain. During the 8-year period from January 1989 to December 1996, 41 patients had VP shunts inserted for PHH at Red Cross War Memorial Children's Hospital. Data on 36 patients were sufficient for review in order to determine whether the timing of surgery in any way influenced the complication incidence of this intervention. Nine of the 36 patients had a serious complication, either infection or mechanical shunt obstruction, during their initial hospital admission (early period). Nineteen patients had surgery performed before day 35 after birth and all those with early complications were in this group. Seventeen patients had surgery delayed until after day 35, and none of these patients had an early complication. In this study a higher complication incidence was noted when a VP shunt was inserted prior to day 35 (Chi-square test P < 0.01). This most probably correlates with a high concentration of blood breakdown products in the cerebrospinal fluid during the first month after intra-ventricular haemorrhage.

Age Factors↗
PubMed2001

Static magnetic fields for treatment of fibromyalgia: a randomized controlled trial.

OBJECTIVE: To test effectiveness of static magnetic fields of two different configurations, produced by magnetic sleep pads, as adjunctive therapies in decreasing patient pain perception and improving functional status in individuals with fibromyalgia. DESIGN: Randomized, placebo-controlled, 6-month trial conducted from November 1997 through December 1998. SETTING AND SUBJECTS: Adults who met the 1990 American College of Rheumatology criteria for fibromyalgia were recruited through clinical referral and media announcements and evaluated at a university-based clinic. INTERVENTIONS: Subjects in Functional Pad A group used a pad for 6 months that provided whole-body exposure to a low, uniform static magnetic field of negative polarity. Subjects in the Functional Pad B group used a pad for 6 months that exposed them to a low static magnetic field that varied spatially and in polarity. Subjects in two Sham groups used pads that were identical in appearance and texture to the functional pads but contained inactive magnets; these groups were combined for analysis. Subjects in the Usual Care group continued with their established treatment regimens. OUTCOME MEASURES: Primary outcomes were the change scores at 6 months in the following measures: functional status (Fibromyalgia Impact Questionnaire), pain intensity ratings, tender point count, and a tender point pain intensity score. RESULTS: There was a significant difference among groups in pain intensity ratings (p = 0.03), with Functional Pad A group showing the greatest reduction from baseline at 6 months. All four groups showed a decline in number of tender points, but differences among the groups were not significant (p = 0.72). The functional pad groups showed the largest decline in total tender point pain intensity, but overall differences were not significant (p = 0.25). Improvement in functional status was greatest in the functional pad groups, but differences among groups were not significant (p = 0.23). CONCLUSIONS: Although the functional pad groups showed improvements in functional status, pain intensity level, tender point count, and tender point intensity after 6 months of treatment, with the exception of pain intensity level these improvements did not differ significantly from changes in the Sham group or in the Usual Care group.

Adolescent↗
PubMed2001

Use of binaural beat tapes for treatment of anxiety: a pilot study of tape preference and outcomes.

CONTEXT: Recent studies and anecdotal reports suggest that binaural auditory beats can affect mood, performance on vigilance tasks, and anxiety. OBJECTIVE: To determine whether mildly anxious people would report decreased anxiety after listening daily for 1 month to tapes imbedded with tones that create binaural beats, and whether they would show a definite tape preference among 3 tapes. DESIGN: A 1-group pre-posttest pilot study. SETTING: Patients' homes. PARTICIPANTS: A volunteer sample of 15 mildly anxious patients seen in the Clinique Psyché, Montreal, Quebec. INTERVENTION: Participants were asked to listen at least 5 times weekly for 4 weeks to 1 or more of 3 music tapes containing tones that produce binaural beats in the electroencephalogram delta/theta frequency range. Participants also were asked to record tape usage, tape preference, and anxiety ratings in a journal before and after listening to the tape or tapes. MAIN OUTCOME MEASURES: Anxiety ratings before and after tape listening, pre- and post-study State-Trait Anxiety Inventory scores, and tape preferences documented in daily journals. RESULTS: Listening to the binaural beat tapes resulted in a significant reduction in the anxiety score reported daily in patients' diaries. The number of times participants listened to the tapes in 4 weeks ranged from 10 to 17 (an average of 1.4 to 2.4 times per week) for approximately 30 minutes per session. End-of-study tape preferences indicated that slightly more participants preferred tape B, with its pronounced and extended patterns of binaural beats, over tapes A and C. Changes in pre- and posttest listening State-Trait Anxiety Inventory scores trended toward a reduction of anxiety, but these differences were not statistically significant. CONCLUSIONS: Listening to binaural beat tapes in the delta/theta electroencephalogram range may be beneficial in reducing mild anxiety. Future studies should account for music preference among participants and include age as a factor in outcomes, incentives to foster tape listening, and a physiologic measure of anxiety reduction. A controlled trial that includes binaural beat tapes as an adjunctive treatment to conventional therapy for mild anxiety may be warranted.

Adult↗
PubMed2000

Responsiveness of fibromyalgia clinical trial outcome measures.

OBJECTIVE: To assess the responsiveness of the Fibromyalgia Impact Questionnaire (FIQ), patient ratings of pain intensity, number of tender points, and total tender point pain intensity score to perceived changes in clinical status in patients with fibromyalgia (FM). METHODS: Using data from a randomized placebo controlled study evaluating efficacy of magnetic therapy in patients with FM, the ability of primary outcomes to detect clinically meaningful changes over a 6 month period was assessed by: (1) degree of association between outcome change scores and patient global ratings of symptom change (Spearman rank-order correlations); (2) ability of these scores to discriminate among groups of patients whose perceived health status had changed to varying degrees (ANOVA); (3) ability of these scores, individually and jointly, to discriminate between patients who had reported improvement and those who did not (logistic regression); (4) effect size, standardized response mean, and Guyatt's statistic were calculated to quantify responsiveness. RESULTS: Correlations showed the outcome measures were moderately responsive to perceived symptomatic change. For FIQ, pain intensity ratings and number of tender points, differences in change scores between globally improved and unchanged groups and between globally improved and worsened groups were significant; for total tender point pain intensity, the globally improved differed from worsened group. FIQ outperformed the other measures in discriminating between patients who reported improvement from those who did not. Summary statistics were consistent with discriminatory analyses, indicating the measures were sensitive to improvement, but relatively unresponsive to decline. CONCLUSION: The FIQ was the most responsive measure to perceived clinical improvement and we recommend its inclusion as a primary endpoint in FM clinical trials.

Adult↗
PubMed1999

Sensitive high-performance liquid chromatography method for the simultaneous determination of low levels of dichloroacetic acid and its metabolites in blood and urine.

Dichloroacetic acid (DCA) is a contaminant found in treated drinking water due to chlorination. DCA has been shown to be a complete hepatocarcinogen in both mice and rats. In this study we developed a rapid and sensitive high-performance liquid chromatography (HPLC) method to simultaneously detect DCA and its metabolites, oxalic acid, glyoxylic acid and glycolic acid in blood and urine samples of animals sub-chronically administered with DCA (2 g/l) in drinking water. Both urine and plasma samples were treated minimally before HPLC analysis. Separation and detection of DCA and its metabolites were achieved using an anion-exchange column and a conductivity detector. The mobile phase consisted of an initial concentration of 0.01 mM sodium hydroxide in 40% methanol followed by a linear gradient from 0.01 mM to 60 mM sodium hydroxide in 40% methanol for 30 min. The lower detection limit for DCA and each of its three major metabolites was 0.05 microg/ml. DCA and its metabolites gave a linear response range from 0.05 to 100 microg/ml. Plasma DCA was also analyzed by gas chromatography (GC), and the results obtained correlated with those from the HPLC method (correlation coefficient=0.999). While available HPLC techniques offer sensitive procedures to detect either glycolic acid or oxalic acid, the described HPLC method has the unique advantage of determining simultaneously the parent compound (DCA) and its three major metabolites (oxalic acid, glyoxylic acid and glycolic acid) in biological samples, without complex sample preparation.

Animals↗
PubMed1999

Complementary and alternative medicine and psychologic factors: toward an individual differences model of complementary and alternative medicine use and outcomes.

CONTEXT: Complementary and alternative medicine (CAM) is in widespread use. Study of the psychologic factors associated with CAM use may provide descriptions of subgroups of health care consumers and give further insight into the reasons for using alternative therapies. OBJECTIVES: To see if the use of CAM is associated with psychologic factors in two outpatient samples and one community sample. SETTING: The University of Virginia Hospital and Charlottesville community. PATIENTS: Patients at the Cancer Center (60) and Pain Management Center (63) and adults from the Charlottesville community (63) for a total of 186 participants. DESIGN: A survey of CAM with psychologic assessment. MAIN MEASURES: Level of CAM use, effectiveness ratings of CAM, positive affect, negative affect, and the Absorption Scale, which measures a personality factor associated with the capacity for focused attention and mind-body awareness. RESULTS: Participants using six or fewer CAM scored an average of 13.9 on the Absorption Scale compared to participants using seven or more CAM, who scored an average of 21.4 on Absorption (F = 48.73, p < .0001). Higher effectiveness ratings of CAM were associated with greater positive affect. Pain Center patients rated alternative therapies as least effective, and reported lower positive affect and greater negative affect compared with the Cancer Center patients and community adults. CONCLUSIONS: Above average use of CAM is associated with higher education, greater well-being and higher Absorption. The personality traits associated with alternative therapies are compatible with participation in these therapies and may facilitate the capacity to benefit from them.

Adult↗
PubMed1998

ED staff members' personal use of complementary therapies and their recommendations to ED patients: a southeastern US regional survey.

BACKGROUND: Use of complementary and alternative therapies has increased in the United States during the past 5 years. Little is known about the use of these therapies in emergency departments. METHODS: The Center for the Study of Complementary and Alternative Therapies, University of Virginia, surveyed staff in 10 emergency departments in the southeast region of the United States with the purpose of exploring ED practitioners' personal use of complementary therapies and recommendations of these therapies to patients. RESULTS: ED staff reported back rub or massage, music, and prayer or spiritual practices as the 3 most frequently used complementary therapies for personal well-being. Back rub or massage and spiritual practices including prayer and group support were most frequently recommended to patients. Clinicians expressed interest in acquiring additional knowledge of complementary therapies and support for integration of these therapies in emergency departments. CONCLUSIONS: Use of complementary therapies for personal well-being and for patient care by ED staff in the southeast region of the United States is limited. Most ED staff are not familiar with these therapies, but a majority of staff (70%) want to learn more about them. Findings suggest that ED nurses would like additional training in, and better referral patterns to, complementary therapies.

Adult↗
PubMed1997

Patients with retained transcranial knife blades: a high-risk group.

Sixty-six patients with transcranial stab wounds presenting to Groote Schuur Hospital over a 2-year period are reviewed. Two groups were identified, those with retained knife blades at presentation (Group A, 13 patients) and those without (Group B, 53 patients). An increased incidence of vascular complications was observed in Group A, (eight of 13 patients) compared with 11 of 53 patients in Group B (p < 0.01, chi-square test). The mortality rate was also higher in Group A, with deaths in three of 13 patients compared with four of 53 in Group B. Increased mortality was a result of vascular injury, and in two patients neurological deterioration occurred only after knife blade removal. Possible reasons for these findings are that retained blades tend to be deeply penetrating with a potential for more cerebral and vascular injury, and there is a higher incidence of petrous bone penetration that results in carotid artery injury.

Aneurysm, False↗
PubMed1993

Phylogenic homogeneity of Coxiella burnetii strains as determinated by 16S ribosomal RNA sequencing.

DNA coding for the 16S rRNA of six strains of the obligate intracellular bacterium Coxiella burnetii was directly amplified from lysed host cells using the polymerase chain reaction. The amplification product was sequenced using a linear-PCR procedure and compared with other published 16S rRNA sequences. The results of this analysis confirm the position of C. burnetii in the gamma subgroup of the proteobacteria. The data show that all of the C. burnetii strains are highly related (> 99%) on the basis of 16S rRNA sequences although they had different geographic origins and phenotypic characteristics. The data support a phylogenetic homogeneity of the genus Coxiella with only one species which is C. burnetii.

Base Sequence↗
PubMed1993

Cat scratch disease and bacillary angiomatosis: aetiological agents and the link with AIDS.

The recent characterisation of a new species of the genus Rochalimaea is the culmination of a ten year search for the identity of the agent of bacillary angiomatosis, a condition that is increasingly recognised in association with AIDS. This work has also implicated Rochalimaea henselae in the aetiology of cat scratch disease contact with cats is a risk factor for both conditions. This report describes both diseases and reviews progress toward understanding their microbiology. R. quintana is also implicated as an agent of bacillary angiomatosis and was recognised much earlier as the cause of trench fever.

Acquired Immunodeficiency Syndrome↗
PubMed1992

Further evidence that genotypically closely related strains of Legionella pneumophila can express different serogroup specific antigens.

The relationship between serogroup and genotype of Legionella pneumophila strains was investigated by restriction fragment length polymorphism (RFLP) typing with a previously standardised method. Of the 51 RFLP types identified, 19 comprised strains of more than one serogroup. Several RFLP types included strains of five or more serogroups. To determine if sharing the same RFLP type indicates that strains are genotypically indistinguishable or merely that they are superficially similar, 31 strains were selected for further analysis with an extended range of restriction endonucleases and nucleic acid probes. In some cases, strains of a particular RFLP type were indistinguishable, while in others the restriction fragment patterns showed minor differences. It is possible that in the latter case the strains are diverging representatives of a parent clone. We conclude that analysis of restriction fragment patterns, either probed or unprobed, provides a more accurate measure of the ancestral relationship between strains than can be obtained with serological methods.

Antigens, Bacterial↗