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Biomedical subjects

A G Rumley

Publications and source records attributed to A G Rumley.

27 records · Page 2Linked to original sources
PubMed1985

Serum lactate dehydrogenase and creatine kinase during marathon training.

Serum total creatine kinase (CK) and the lactate dehydrogenase (LDH) isoenzymes were studied in 38 sedentary middle-aged men (aged 35-50 yrs) during a 30 week marathon training programme. Basal CK activity rose by 33% after 15 weeks but a significant rise (27%) in LDH activity took 30 weeks to occur. Post-exercise (maximum test on a bicycle ergometer) CK and LDH activities were higher than pre-exercise levels but the increment in enzyme activity following exercise did not change. LDH1 and LDH2 isoenzyme activity increased by 2.5% and 4% of total LDH respectively while LDH3 and LDH5 decreased by 3.9% and 2.4% respectively over 30 weeks. Post marathon total CK did not correlate with finishing time at 30 mins or 30 hrs post race. The range of CK MB isoenzyme activity at 30 mins post race was 1.8-9.8% of total CK with 11 subjects having a value above 6%. The training programme appears not to have affected muscle CK and LDH release during exercise but isoenzyme distribution changes reflect the adaptations known to occur in muscle during endurance training. Unfortunately only 16 subjects were available for all the investigations, and it is these upon whom most of the data were obtained.

Adult↗
PubMed1985

Catecholamines in human aqueous humor.

Aqueous humor catecholamine levels were measured in 14 patients admitted to hospital for cataract or glaucoma surgery. Norepinephrine was detected in all patients. Epinephrine was detected in one patient who had received a preoperative retrobulbar injection of epinephrine. Dopamine was not detected in any patients. The highest level of norepinephrine was detected in cataract patients with normal intraocular pressures (mean = 7.18 nmol/l).

Aged↗
PubMed1984

Catecholamine concentrations in tears.

Lacrimal fluid from 17 healthy volunteers was analysed for catecholamines using a radioenzymatic assay. Significant concentrations of norepinephrine (mean = 4.4 nmol l-1) and epinephrine (mean = 3.7 nmol l-1) were detected in some of the subjects. Dopamine was not detected in the tears of any of the subjects. The possible significance of catecholamines in tears in relation to corneal physiology and intraocular pressure is discussed.

Adult↗
PubMed1982

Effect of favourable treatment of samples on indices of performance in external quality assessment schemes.

This study was carried out to examine the effect on performance, as assessed by external quality assessment schemes, of (a) replicate analysis of external quality assessment samples, and positioning of external quality assessment samples immediately after the calibrants, and (b) alteration of results on the basis of previous performance in order to bring them closer to the method mean values obtained from all laboratories participating in the external quality assessment scheme. Indices of performance were then compared with those obtained from our normal practice where external quality assessment samples were treated as patient samples. Using these methods we were unable to improve significantly our precision or league ranking based on precision over the six-month period of study. We were able to reduce our bias from method means but at the expense of making precision and league ranking worse.

Chemistry, Clinical↗
PubMed1980

Comparison of digoxin analysis by EMIT with Immophase and Dac-Cel radioimmunoassay.

The results obtained by an enzyme multiplied immunoassay kit, EMIT, for the measurement of serum digoxin concentration were compared with those from two radioimmunoassay kit methods, Immophase and Dac-Cel. Patients' sera were used to study the correlation between the methods, and three quality control sera with low, intermediate, and high digoxin concentrations were used to study individual method precision. The coefficients of correlation between the three methods varied between 0.915 and 0.951 for serum drug concentrations up to 4.5 nmol/l. There were statistically significant differences between the means of the patients' samples for each method. Precision was acceptable for each method: within-batch percentage coefficient of variation (%CV) less than 8, between-batch %CV less than 10, except for Dac-Cel at low concentration %CV = 18.6. The time taken for the analysis of a batch of 10 samples was between 1.5 and 2 hours, depending on the method.

Digoxin↗